Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 17 | 33 | 37.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GFKOKENE_12 | 58224 | 58817 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| GFKOKENE_12 | 58224 | 58817 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| GFKOKENE_12 | 58224 | 58817 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| GFKOKENE_12 | 57355 | 58224 | rfbA | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011681286 |
| GFKOKENE_55 | 65463 | 67121 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| GFKOKENE_12 | 112983 | 113772 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.11 | 99.87 | - |
| GFKOKENE_12 | 178906 | 179637 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | WP_011681176 |
| GFKOKENE_12 | 22690 | 23451 | srtA | 0.0 | Sortase A | Exoenzyme | 98.95 | 100.00 | - |
| GFKOKENE_12 | 178906 | 179637 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.91 | 100.00 | WP_011226048 |
| GFKOKENE_12 | 178906 | 179637 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011227248 |
| GFKOKENE_12 | 180348 | 181088 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.52 | 100.00 | WP_011681174 |
| GFKOKENE_12 | 177445 | 178905 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011681177 |
| GFKOKENE_12 | 180348 | 181088 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 97.71 | 100.00 | WP_011226046 |
| GFKOKENE_12 | 180348 | 181083 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.55 | 98.13 | WP_011227246 |
| GFKOKENE_1 | 158937 | 159897 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.32 | 99.90 | WP_082302295 |
| GFKOKENE_12 | 177445 | 178905 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.78 | 100.21 | WP_011226049 |
| GFKOKENE_76 | 31097 | 31948 | rfbD | 0.0 | Capsule | Immune modulation | 96.71 | 100.00 | WP_014621820 |
| GFKOKENE_12 | 177445 | 178905 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.51 | 100.21 | WP_041827043 |
| GFKOKENE_12 | 181145 | 182512 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.10 | 100.00 | WP_011226045 |
| GFKOKENE_12 | 181145 | 182510 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.22 | 99.85 | WP_011681173 |
| GFKOKENE_12 | 179646 | 180338 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.06 | 99.86 | WP_011681175 |
| GFKOKENE_1 | 183 | 1486 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.02 | 99.46 | WP_000022832 |
| GFKOKENE_10 | 25445 | 26455 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.73 | 99.51 | WP_002986042 |
| GFKOKENE_12 | 179646 | 180338 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 86.02 | 99.86 | WP_011226047 |
| GFKOKENE_12 | 179646 | 180338 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 85.88 | 99.86 | WP_011227247 |
| GFKOKENE_16 | 3528 | 4811 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| GFKOKENE_12 | 59246 | 60292 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
| GFKOKENE_12 | 192680 | 193263 | SPD_RS01775 | 5.44e-146 | Capsule | Immune modulation | 82.91 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GFKOKENE_12 | 20003 | 19020 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| GFKOKENE_44 | 67784 | 66711 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| GFKOKENE_1 | 83642 | 82674 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| GFKOKENE_33 | 83428 | 81125 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.84 | 99.22 | AAK74619 |
| GFKOKENE_5 | 22137 | 23618 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| GFKOKENE_44 | 39357 | 37111 | metE | 0.0 | methionine biosynthesis enzymes | 91.59 | 100.00 | AAK74738 |
| GFKOKENE_7 | 1929 | 937 | recA | 0.0 | DNA recombination and repair | 91.24 | 85.31 | AAL00560 |
| GFKOKENE_6 | 33551 | 32580 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| GFKOKENE_76 | 80910 | 79759 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| GFKOKENE_76 | 45136 | 43148 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| GFKOKENE_12 | 36202 | 37932 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| GFKOKENE_77 | 27867 | 29345 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| GFKOKENE_1 | 1486 | 185 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| GFKOKENE_77 | 42600 | 44492 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| GFKOKENE_44 | 34186 | 35895 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| GFKOKENE_66 | 21516 | 22526 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| GFKOKENE_55 | 92525 | 91164 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| GFKOKENE_66 | 71779 | 69887 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| GFKOKENE_1 | 15926 | 15036 | bglB | 1.57e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| GFKOKENE_55 | 19297 | 18359 | SP_0676 | 9.30e-169 | transcription facot | 81.15 | 97.20 | AAK74821 |
| GFKOKENE_44 | 36919 | 36062 | metF | 1.34e-165 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| GFKOKENE_12 | 141407 | 142111 | covR | 4.53e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| GFKOKENE_2 | 38569 | 37898 | rr01 | 1.29e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| GFKOKENE_12 | 141407 | 142111 | walR | 2.12e-122 | DNA-binding response regulator | 81.70 | 100.00 | ANC99504 |
| GFKOKENE_44 | 30232 | 29564 | CiaR | 4.77e-121 | two-component response regulator | 85.65 | 99.55 | AAK74935 |
| GFKOKENE_44 | 30232 | 29561 | ciaR | 5.70e-119 | two component system response regulator | 83.48 | 99.12 | AIK75708 |
| GFKOKENE_23 | 138181 | 138867 | CovR | 1.02e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| GFKOKENE_7 | 640 | 245 | SpxA2 | 7.02e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| GFKOKENE_55 | 103946 | 104347 | SpxA1 | 2.79e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| GFKOKENE_23 | 160607 | 160894 | spr0479 | 1.31e-48 | essential gene | 86.46 | 98.97 | AAK99283 |
| GFKOKENE_1 | 118051 | 117797 | spr1327 | 3.27e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| GFKOKENE_77 | 76238 | 75975 | spr0175 | 3.42e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| GFKOKENE_77 | 75411 | 75145 | spr0177 | 1.03e-33 | essential gene | 84.27 | 88.12 | AAK98981 |
| GFKOKENE_16 | 3370 | 2870 | RopE (M5005_Spy_1611) | 1.65e-77 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| GFKOKENE_33 | 83428 | 81131 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.81 | 98.08 | MYN69560 |
| GFKOKENE_66 | 57380 | 56361 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GFKOKENE_6 | 723 | 1670 | rep37 | 950 / 948 | 94.84 | AF177167 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GFKOKENE_1 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_2 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_3 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_4 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_5 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_6 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_7 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_8 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_9 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_10 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_11 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_12 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_13 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_14 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_15 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_16 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_18 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_19 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_20 | 1.0 | 0.0 | 1.0 |
| GFKOKENE_21 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_23 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_24 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_25 | 1.0 | 0.0 | 1.0 |
| GFKOKENE_33 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_44 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_55 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_66 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_76 | 0.0 | 1.0 | 0.0 |
| GFKOKENE_77 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)