Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 16 | 33 | 36.67 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KIOLHBIL_12 | 86504 | 87097 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| KIOLHBIL_12 | 86504 | 87097 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| KIOLHBIL_12 | 86504 | 87097 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| KIOLHBIL_12 | 85635 | 86504 | rfbA | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011681286 |
| KIOLHBIL_45 | 60392 | 62050 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| KIOLHBIL_50 | 60630 | 62084 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 99.31 | 100.00 | WP_011226049 |
| KIOLHBIL_12 | 141263 | 142052 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.11 | 99.87 | - |
| KIOLHBIL_12 | 50970 | 51731 | srtA | 0.0 | Sortase A | Exoenzyme | 98.95 | 100.00 | - |
| KIOLHBIL_50 | 63527 | 64267 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_011681174 |
| KIOLHBIL_50 | 63527 | 64267 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.65 | 100.00 | WP_011226046 |
| KIOLHBIL_50 | 60630 | 62084 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.49 | 100.00 | WP_041827043 |
| KIOLHBIL_50 | 62085 | 62816 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.09 | 100.00 | WP_011226048 |
| KIOLHBIL_50 | 63527 | 64262 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 97.96 | 98.13 | WP_011227246 |
| KIOLHBIL_50 | 62085 | 62816 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681176 |
| KIOLHBIL_50 | 62085 | 62816 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.40 | 100.00 | WP_011227248 |
| KIOLHBIL_53 | 37025 | 37985 | cbpD | 0.0 | Autolysin | Exoenzyme | 97.32 | 99.90 | WP_082302295 |
| KIOLHBIL_50 | 60630 | 62084 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.06 | 99.79 | WP_011681177 |
| KIOLHBIL_52 | 25299 | 26150 | rfbD | 0.0 | Capsule | Immune modulation | 96.71 | 100.00 | WP_014621820 |
| KIOLHBIL_50 | 62825 | 63517 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 96.54 | 100.00 | WP_011227247 |
| KIOLHBIL_50 | 62825 | 63517 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 96.54 | 100.00 | WP_011226047 |
| KIOLHBIL_50 | 64325 | 65688 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.16 | 99.71 | WP_011226045 |
| KIOLHBIL_50 | 64325 | 65692 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.52 | 100.00 | WP_011681173 |
| KIOLHBIL_50 | 62825 | 63517 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.19 | 100.00 | WP_011681175 |
| KIOLHBIL_2 | 32413 | 33716 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 87.94 | 99.54 | WP_000022832 |
| KIOLHBIL_9 | 25913 | 26923 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.73 | 99.51 | WP_002986042 |
| KIOLHBIL_1 | 198739 | 200022 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| KIOLHBIL_12 | 87526 | 88572 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KIOLHBIL_12 | 48283 | 47300 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| KIOLHBIL_23 | 42955 | 44028 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| KIOLHBIL_51 | 65719 | 64751 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| KIOLHBIL_6 | 804 | 3107 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.84 | 99.22 | AAK74619 |
| KIOLHBIL_3 | 31930 | 30449 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.70 | 100.00 | ABJ54480 |
| KIOLHBIL_8 | 38826 | 36580 | metE | 0.0 | methionine biosynthesis enzymes | 91.59 | 100.00 | AAK74738 |
| KIOLHBIL_5 | 66770 | 67768 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| KIOLHBIL_5 | 3505 | 4476 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| KIOLHBIL_34 | 120249 | 121400 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.32 | 99.74 | AAL00050 |
| KIOLHBIL_52 | 12123 | 14111 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| KIOLHBIL_12 | 64482 | 66212 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| KIOLHBIL_23 | 84893 | 86371 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| KIOLHBIL_2 | 32413 | 33714 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| KIOLHBIL_23 | 99627 | 101519 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| KIOLHBIL_8 | 33655 | 35364 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| KIOLHBIL_4 | 57435 | 56425 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| KIOLHBIL_45 | 87454 | 86093 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| KIOLHBIL_4 | 7172 | 9064 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| KIOLHBIL_2 | 17974 | 18864 | bglB | 6.85e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| KIOLHBIL_45 | 11910 | 10975 | SP_0676 | 5.26e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| KIOLHBIL_8 | 36388 | 35531 | metF | 4.07e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| KIOLHBIL_50 | 24507 | 25211 | covR | 1.91e-132 | responder protein | 85.53 | 100.00 | AAK71319 |
| KIOLHBIL_3 | 78120 | 78791 | rr01 | 1.97e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| KIOLHBIL_50 | 24507 | 25211 | walR | 6.81e-123 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KIOLHBIL_8 | 29701 | 29033 | CiaR | 1.81e-121 | two-component response regulator | 85.65 | 99.55 | AAK74935 |
| KIOLHBIL_8 | 29701 | 29030 | ciaR | 2.95e-119 | two component system response regulator | 83.48 | 99.12 | AIK75708 |
| KIOLHBIL_1 | 50175 | 49489 | CovR | 1.13e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| KIOLHBIL_5 | 68044 | 68439 | SpxA2 | 1.37e-75 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| KIOLHBIL_45 | 98875 | 99276 | SpxA1 | 2.69e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| KIOLHBIL_1 | 27762 | 27475 | spr0479 | 1.45e-48 | essential gene | 86.46 | 98.97 | AAK99283 |
| KIOLHBIL_51 | 99804 | 99550 | spr1327 | 1.54e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| KIOLHBIL_23 | 133256 | 132993 | spr0175 | 5.62e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| KIOLHBIL_23 | 132429 | 132163 | spr0177 | 1.69e-33 | essential gene | 84.27 | 88.12 | AAK98981 |
| KIOLHBIL_1 | 198581 | 198081 | RopE (M5005_Spy_1611) | 8.81e-75 | probable DNA-directed RNA polymerase subunit delta | 80.84 | 87.43 | AAK34603 |
| KIOLHBIL_6 | 804 | 3101 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.81 | 98.08 | MYN69560 |
| KIOLHBIL_4 | 21571 | 22590 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| KIOLHBIL_19 | 1788 | 2735 | rep37 | 950 / 948 | 94.84 | AF177167 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KIOLHBIL_1 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_2 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_3 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_4 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_5 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_6 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_7 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_8 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_9 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_10 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_11 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_12 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_13 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_14 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_15 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_16 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_17 | 1.0 | 0.0 | 1.0 |
| KIOLHBIL_19 | 1.0 | 0.0 | 1.0 |
| KIOLHBIL_20 | 1.0 | 0.0 | 1.0 |
| KIOLHBIL_21 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_22 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_23 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_34 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_45 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_50 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_51 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_52 | 0.0 | 1.0 | 0.0 |
| KIOLHBIL_53 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KIOLHBIL_11 | 1696 | 24173 | False | Siphoviridae | VOG0283, VOG0685, VOG0226, VOG4693, VOG0655, VOG3468, VOG0656, VOG6414, VOG0152, VOG6495, VOG0658, VOG0198, VOG0796, VOG4544, VOG0720, VOG4564, VOG4555, VOG3434, VOG1887, VOG3435, VOG4713, VOG0723, VOG5660, VOG0724, VOG0799, VOG0725 |
| KIOLHBIL_50 | 4584 | 10558 | False | Siphoviridae | VOG3298, VOG3424, VOG0054, VOG4649, VOG3299, VOG3300, VOG3301, VOG3436, VOG3303, VOG4134 |
Phigaro v2.4.0 (Database: Jan. 2024 release)