Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IPFIMMAO_1 | 4031208 | 4032128 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| IPFIMMAO_1 | 4017894 | 4019327 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| IPFIMMAO_1 | 3968256 | 3968849 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| IPFIMMAO_1 | 3726071 | 3727714 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| IPFIMMAO_1 | 3392097 | 3394667 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| IPFIMMAO_1 | 2931808 | 2932146 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IPFIMMAO_1 | 2931491 | 2931808 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IPFIMMAO_1 | 1743401 | 1744570 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| IPFIMMAO_1 | 1516252 | 1517454 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| IPFIMMAO_1 | 1497939 | 1498793 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IPFIMMAO_1 | 28073 | 29449 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.84 | 100.00 | X08034 |
| IPFIMMAO_1 | 1497939 | 1498791 | aadK | Streptomycin | 98.71 | 99.77 | M26879 |
| IPFIMMAO_1 | 4031208 | 4032128 | mph(K) | Spiramycin, Telithromycin | 97.72 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IPFIMMAO_1 | 28073 | 29446 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.69 | 100.00 | WP_003242953.1 |
| IPFIMMAO_1 | 3726071 | 3727711 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| IPFIMMAO_1 | 1497939 | 1498790 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| IPFIMMAO_1 | 4031211 | 4032128 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| IPFIMMAO_1 | 31402 | 31920 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| IPFIMMAO_1 | 2075584 | 2078181 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IPFIMMAO_1 | 1945373 | 1946014 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| IPFIMMAO_1 | 920853 | 921791 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| IPFIMMAO_1 | 513058 | 514239 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IPFIMMAO_1 | 919206 | 920825 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.26 | 100.00 | NP_391078 |
| IPFIMMAO_1 | 917170 | 917955 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| IPFIMMAO_1 | 514722 | 515864 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| IPFIMMAO_1 | 917981 | 919177 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| IPFIMMAO_1 | 2123359 | 2125122 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 100.00 | NP_389723 |
| IPFIMMAO_1 | 921811 | 928947 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.82 | 100.00 | NP_391076 |
| IPFIMMAO_1 | 514254 | 514703 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IPFIMMAO_1 | 4174766 | 4173630 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IPFIMMAO_1 | 4288499 | 4287630 | pdxS | 3.20e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IPFIMMAO_1 | 2621961 | 2621185 | CodY | 3.49e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IPFIMMAO_1 | 655656 | 655087 | ClpP | 2.59e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IPFIMMAO_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IPFIMMAO_1 | 1534302 | 1566591 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG4567, VOG4673, VOG7060, VOG0796, VOG9315, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4691, VOG4862, VOG7718 |
| IPFIMMAO_1 | 2036106 | 2058724 | False | Siphoviridae | VOG6154, VOG0519, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG5660, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641 |
| IPFIMMAO_1 | 2260120 | 2290666 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG4545, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544, VOG4581, VOG4841, VOG8507, VOG0198, VOG7372, VOG2752 |
| IPFIMMAO_1 | 2961630 | 2989194 | False | Myoviridae | VOG0753, VOG4705, VOG4612, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| IPFIMMAO_1 | 3640377 | 3664998 | False | Myoviridae | VOG2703, VOG4574, VOG0136, VOG0753, VOG5008, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG1350, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG0815, VOG4553, VOG4555, VOG0720, VOG0796 |
| IPFIMMAO_1 | 3671291 | 3680205 | False | Siphoviridae | VOG0189, VOG4566, VOG0186, VOG8233, VOG4603, VOG4552, VOG0650, VOG11075, VOG9667, VOG4602 |
Phigaro v2.4.0 (Database: Jan. 2024 release)