Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 17 | 34 | 38.01 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LEJACOOO_17 | 128 | 967 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| LEJACOOO_23 | 61155 | 61748 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| LEJACOOO_23 | 61155 | 61748 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| LEJACOOO_23 | 61155 | 61748 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| LEJACOOO_24 | 10927 | 12585 | fbp54 | 0.0 | FBPs | Adherence | 99.58 | 100.00 | WP_011227179 |
| LEJACOOO_23 | 60286 | 61155 | rfbA | 0.0 | Capsule | Immune modulation | 99.19 | 100.00 | WP_011681286 |
| LEJACOOO_12 | 13748 | 14479 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.18 | 100.00 | WP_011227248 |
| LEJACOOO_23 | 105800 | 106590 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 100.00 | - |
| LEJACOOO_1 | 30638 | 31489 | rfbD | 0.0 | Capsule | Immune modulation | 98.71 | 100.00 | WP_014621820 |
| LEJACOOO_23 | 25628 | 26389 | srtA | 0.0 | Sortase A | Exoenzyme | 98.69 | 100.00 | - |
| LEJACOOO_12 | 15190 | 15930 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 98.52 | 100.00 | WP_011681174 |
| LEJACOOO_12 | 13748 | 14479 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.50 | 100.00 | WP_011226048 |
| LEJACOOO_12 | 15190 | 15930 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.25 | 100.00 | WP_011226046 |
| LEJACOOO_12 | 13748 | 14479 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011681176 |
| LEJACOOO_12 | 15190 | 15925 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.10 | 98.13 | WP_011227246 |
| LEJACOOO_12 | 12287 | 13747 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681177 |
| LEJACOOO_12 | 12287 | 13747 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.92 | 100.21 | WP_011226049 |
| LEJACOOO_11 | 19115 | 24010 | SAG_RS09105 | 0.0 | Agglutinin receptor | Adherence | 96.89 | 100.00 | WP_000167699 |
| LEJACOOO_12 | 12287 | 13747 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.10 | 100.21 | WP_041827043 |
| LEJACOOO_11 | 19067 | 24010 | SSU98_0978 | 0.0 | Agglutinin receptor | Adherence | 95.73 | 99.82 | YP_001200536 |
| LEJACOOO_11 | 19067 | 24010 | SSU05_0965 | 0.0 | Agglutinin receptor | Adherence | 95.71 | 99.82 | YP_001198331 |
| LEJACOOO_12 | 15988 | 17355 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 95.47 | 100.00 | WP_011226045 |
| LEJACOOO_12 | 15988 | 17353 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.80 | 99.85 | WP_011681173 |
| LEJACOOO_12 | 14488 | 15180 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011226047 |
| LEJACOOO_12 | 14488 | 15180 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 90.62 | 100.00 | WP_011227247 |
| LEJACOOO_12 | 14488 | 15180 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 89.18 | 100.00 | WP_011681175 |
| LEJACOOO_7 | 14457 | 15760 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.17 | 99.54 | WP_000022832 |
| LEJACOOO_22 | 14293 | 15303 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.82 | 99.51 | WP_002986042 |
| LEJACOOO_4 | 2112 | 3395 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| LEJACOOO_23 | 62177 | 63223 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
| LEJACOOO_1 | 26028 | 26495 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 94.46 | 65.27 | WP_011681442 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LEJACOOO_11 | 19094 | 24007 | B9H01_RS04765 | 0.0 | glucan-binding protein | 96.22 | 99.70 | AUS90958 |
| LEJACOOO_23 | 22941 | 21958 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| LEJACOOO_34 | 13655 | 12582 | MntE | 0.0 | manganese homeostasis | 93.86 | 99.72 | ABF32167 |
| LEJACOOO_1 | 186396 | 188699 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.23 | 99.22 | AAK74619 |
| LEJACOOO_51 | 41866 | 42834 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| LEJACOOO_20 | 11320 | 9074 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| LEJACOOO_52 | 3215 | 4696 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.70 | 100.00 | ABJ54480 |
| LEJACOOO_17 | 19758 | 20756 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| LEJACOOO_19 | 15255 | 14284 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| LEJACOOO_1 | 72558 | 71407 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| LEJACOOO_1 | 44557 | 42569 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| LEJACOOO_23 | 39137 | 40867 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| LEJACOOO_54 | 27003 | 28481 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| LEJACOOO_7 | 15760 | 14459 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| LEJACOOO_54 | 42228 | 44120 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| LEJACOOO_20 | 6149 | 7858 | PGM | 0.0 | phosphoglucomutase | 84.06 | 99.65 | ACH87041 |
| LEJACOOO_53 | 20067 | 21077 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| LEJACOOO_12 | 103326 | 104687 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| LEJACOOO_53 | 70331 | 68439 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| LEJACOOO_7 | 30198 | 29308 | bglB | 3.67e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| LEJACOOO_6 | 44553 | 45491 | SP_0676 | 2.46e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| LEJACOOO_20 | 8882 | 8025 | metF | 1.35e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| LEJACOOO_9 | 24381 | 23677 | covR | 1.34e-132 | responder protein | 85.11 | 100.00 | AAK71319 |
| LEJACOOO_3 | 42648 | 41977 | rr01 | 1.70e-125 | response regulator | 83.93 | 99.56 | CAB54566 |
| LEJACOOO_9 | 24381 | 23677 | walR | 6.04e-123 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| LEJACOOO_15 | 7733 | 7047 | CovR | 1.27e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| LEJACOOO_20 | 2195 | 1527 | CiaR | 1.79e-119 | two-component response regulator | 84.31 | 99.55 | AAK74935 |
| LEJACOOO_20 | 2195 | 1524 | ciaR | 1.76e-117 | two component system response regulator | 82.14 | 99.12 | AIK75708 |
| LEJACOOO_17 | 21032 | 21427 | SpxA2 | 4.28e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| LEJACOOO_12 | 91901 | 91500 | SpxA1 | 2.58e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| LEJACOOO_10 | 25405 | 25118 | spr0479 | 2.95e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| LEJACOOO_51 | 7451 | 7705 | spr1327 | 1.31e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| LEJACOOO_27 | 1210 | 947 | spr0175 | 4.73e-43 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| LEJACOOO_27 | 383 | 117 | spr0177 | 1.21e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| LEJACOOO_4 | 3553 | 4053 | RopE (M5005_Spy_1611) | 1.75e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| LEJACOOO_1 | 186396 | 188693 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| LEJACOOO_53 | 55925 | 54906 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LEJACOOO_1 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_2 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_3 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_4 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_5 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_6 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_7 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_8 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_9 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_10 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_11 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_12 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_13 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_14 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_15 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_16 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_17 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_18 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_19 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_20 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_21 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_22 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_23 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_24 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_25 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_26 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_27 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_28 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_29 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_30 | 1.0 | 0.0 | 1.0 |
| LEJACOOO_31 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_32 | 1.0 | 0.0 | 1.0 |
| LEJACOOO_33 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_34 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_35 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_36 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_37 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_38 | 1.0 | 0.0 | 1.0 |
| LEJACOOO_39 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_40 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_41 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_42 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_43 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_44 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_45 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_46 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_47 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_51 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_52 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_53 | 0.0 | 1.0 | 0.0 |
| LEJACOOO_54 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LEJACOOO_14 | 17400 | 19638 | False | Unknown | VOG9667, VOG9405, VOG6396 |
Phigaro v2.4.0 (Database: Jan. 2024 release)