Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 21 | 32 | 38.28 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AFKGALCK_1 | 273542 | 274597 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_002948022 |
| AFKGALCK_1 | 271508 | 272317 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_002948020 |
| AFKGALCK_1 | 274594 | 276378 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | WP_011226325 |
| AFKGALCK_1 | 286809 | 287648 | cbpD | 0.0 | Autolysin | Exoenzyme | 99.88 | 100.00 | WP_011226733 |
| AFKGALCK_1 | 272317 | 273522 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226326 |
| AFKGALCK_1 | 270568 | 271524 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| AFKGALCK_12 | 85509 | 86102 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| AFKGALCK_1 | 269423 | 270571 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_041828290 |
| AFKGALCK_12 | 85509 | 86102 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| AFKGALCK_12 | 85509 | 86102 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| AFKGALCK_34 | 12865 | 14523 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| AFKGALCK_12 | 130138 | 130928 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.11 | 100.00 | - |
| AFKGALCK_12 | 84640 | 85509 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| AFKGALCK_12 | 49980 | 50741 | srtA | 0.0 | Sortase A | Exoenzyme | 98.95 | 100.00 | - |
| AFKGALCK_1 | 265490 | 266764 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.82 | 100.00 | WP_011226331 |
| AFKGALCK_1 | 266764 | 267726 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.75 | 95.25 | WP_011226330 |
| AFKGALCK_13 | 5180 | 6634 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 98.69 | 100.00 | WP_041827043 |
| AFKGALCK_13 | 5180 | 6634 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.59 | 100.00 | WP_011226049 |
| AFKGALCK_13 | 5180 | 6634 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 96.30 | 99.79 | WP_011681177 |
| AFKGALCK_1 | 263538 | 264389 | rfbD | 0.0 | Capsule | Immune modulation | 96.24 | 100.00 | WP_014621820 |
| AFKGALCK_23 | 105957 | 107260 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| AFKGALCK_14 | 173 | 1183 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.82 | 99.51 | WP_002986042 |
| AFKGALCK_2 | 64808 | 66091 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| AFKGALCK_12 | 86531 | 87577 | rfbB | 0.0 | Capsule | Immune modulation | 80.33 | 100.00 | WP_009659423 |
| AFKGALCK_13 | 2213 | 2796 | SPD_RS01775 | 1.09e-148 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
| AFKGALCK_13 | 4715 | 5179 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.28 | 63.52 | WP_011681176 |
| AFKGALCK_13 | 4715 | 5179 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.06 | 63.52 | WP_011226048 |
| AFKGALCK_13 | 4715 | 5179 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 97.85 | 63.52 | WP_011227248 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AFKGALCK_12 | 47293 | 46310 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| AFKGALCK_3 | 40342 | 41415 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| AFKGALCK_1 | 108377 | 106074 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| AFKGALCK_23 | 24762 | 25730 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| AFKGALCK_35 | 65742 | 64261 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| AFKGALCK_8 | 5994 | 8240 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| AFKGALCK_1 | 307310 | 308308 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| AFKGALCK_33 | 109889 | 108918 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.12 | 100.00 | ABF36968 |
| AFKGALCK_1 | 222331 | 223482 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| AFKGALCK_1 | 250331 | 252319 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| AFKGALCK_12 | 63489 | 65219 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| AFKGALCK_37 | 27018 | 28496 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| AFKGALCK_23 | 105957 | 107258 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| AFKGALCK_37 | 42214 | 44106 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| AFKGALCK_8 | 11165 | 9456 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| AFKGALCK_38 | 18828 | 19838 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| AFKGALCK_34 | 38505 | 37144 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| AFKGALCK_23 | 91518 | 92408 | bglB | 4.77e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| AFKGALCK_4 | 40762 | 41697 | SP_0676 | 2.07e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| AFKGALCK_8 | 8432 | 9289 | metF | 1.39e-165 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| AFKGALCK_12 | 162579 | 163283 | covR | 3.53e-131 | responder protein | 84.68 | 100.00 | AAK71319 |
| AFKGALCK_35 | 90341 | 91012 | rr01 | 5.27e-124 | response regulator | 83.04 | 99.56 | CAB54566 |
| AFKGALCK_8 | 15119 | 15787 | CiaR | 1.26e-121 | two-component response regulator | 85.65 | 99.55 | AAK74935 |
| AFKGALCK_12 | 162579 | 163283 | walR | 2.53e-121 | DNA-binding response regulator | 80.85 | 100.00 | ANC99504 |
| AFKGALCK_8 | 15119 | 15790 | ciaR | 1.54e-119 | two component system response regulator | 83.48 | 99.12 | AIK75708 |
| AFKGALCK_33 | 9926 | 9240 | CovR | 6.13e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| AFKGALCK_1 | 308584 | 308958 | SpxA2 | 1.92e-70 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| AFKGALCK_34 | 49864 | 50265 | SpxA1 | 2.09e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| AFKGALCK_6 | 14758 | 15045 | spr0479 | 3.06e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| AFKGALCK_36 | 9896 | 9642 | spr1327 | 1.36e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| AFKGALCK_37 | 75837 | 75574 | spr0175 | 3.97e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| AFKGALCK_37 | 75010 | 74744 | spr0177 | 7.01e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| AFKGALCK_2 | 64650 | 64150 | RopE (M5005_Spy_1611) | 1.80e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| AFKGALCK_1 | 108377 | 106080 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| AFKGALCK_38 | 56373 | 55354 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| AFKGALCK_22 | 2514 | 3143 | rep37 | 632 / 948 | 95.09 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AFKGALCK_1 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_2 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_3 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_4 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_5 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_6 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_7 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_8 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_9 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_10 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_11 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_12 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_13 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_14 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_15 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_16 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_18 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_19 | 1.0 | 0.0 | 1.0 |
| AFKGALCK_20 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_22 | 1.0 | 0.0 | 1.0 |
| AFKGALCK_23 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_24 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_25 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_26 | 1.0 | 0.0 | 1.0 |
| AFKGALCK_27 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_28 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_29 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_33 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_34 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_35 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_36 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_37 | 0.0 | 1.0 | 0.0 |
| AFKGALCK_38 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)