Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 26 | 32 | 41.23 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GGEMHDLO_1 | 11013 | 12068 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_002948022 |
| GGEMHDLO_1 | 13293 | 14102 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_002948020 |
| GGEMHDLO_1 | 9232 | 11016 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | WP_011226325 |
| GGEMHDLO_1 | 12088 | 13293 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226326 |
| GGEMHDLO_1 | 14086 | 15042 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| GGEMHDLO_12 | 61176 | 61769 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| GGEMHDLO_1 | 15039 | 16187 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_041828290 |
| GGEMHDLO_12 | 61176 | 61769 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| GGEMHDLO_17 | 9224 | 9823 | STR_RS05245 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_196767671 |
| GGEMHDLO_12 | 61176 | 61769 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| GGEMHDLO_34 | 89056 | 90714 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| GGEMHDLO_32 | 1 | 1369 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 99.20 | 94.09 | WP_041827043 |
| GGEMHDLO_12 | 105804 | 106594 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.11 | 100.00 | - |
| GGEMHDLO_12 | 60307 | 61176 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| GGEMHDLO_17 | 9974 | 10709 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 99.05 | 99.33 | WP_011681174 |
| GGEMHDLO_12 | 25647 | 26408 | srtA | 0.0 | Sortase A | Exoenzyme | 98.95 | 100.00 | - |
| GGEMHDLO_17 | 9960 | 10709 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.93 | 100.00 | WP_011227246 |
| GGEMHDLO_17 | 11420 | 12029 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.85 | 83.33 | WP_011227248 |
| GGEMHDLO_1 | 18846 | 20120 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.82 | 100.00 | WP_011226331 |
| GGEMHDLO_1 | 17884 | 18846 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.75 | 95.25 | WP_011226330 |
| GGEMHDLO_17 | 11420 | 12029 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.69 | 83.33 | WP_011226048 |
| GGEMHDLO_38 | 39513 | 40481 | cbpD | 0.0 | Autolysin | Exoenzyme | 98.56 | 100.00 | WP_082302295 |
| GGEMHDLO_17 | 9974 | 10709 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.37 | 99.33 | WP_011226046 |
| GGEMHDLO_17 | 8100 | 9059 | STR_RS05240 | 0.0 | Capsule | Immune modulation | 98.23 | 100.00 | WP_041827042 |
| GGEMHDLO_17 | 11420 | 12029 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.20 | 83.33 | WP_011681176 |
| GGEMHDLO_32 | 1 | 1369 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.17 | 94.09 | WP_011226049 |
| GGEMHDLO_17 | 10719 | 11411 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011227247 |
| GGEMHDLO_17 | 10719 | 11411 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011226047 |
| GGEMHDLO_32 | 1 | 1369 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 96.80 | 93.91 | WP_011681177 |
| GGEMHDLO_1 | 21221 | 22072 | rfbD | 0.0 | Capsule | Immune modulation | 96.24 | 100.00 | WP_014621820 |
| GGEMHDLO_17 | 10719 | 11411 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011681175 |
| GGEMHDLO_23 | 106041 | 107344 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| GGEMHDLO_16 | 14258 | 15268 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.82 | 99.51 | WP_002986042 |
| GGEMHDLO_2 | 2047 | 3330 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| GGEMHDLO_12 | 62198 | 63244 | rfbB | 0.0 | Capsule | Immune modulation | 80.33 | 100.00 | WP_009659423 |
| GGEMHDLO_24 | 2213 | 2796 | SPD_RS01775 | 2.83e-149 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GGEMHDLO_12 | 22960 | 21977 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| GGEMHDLO_14 | 13749 | 12676 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| GGEMHDLO_1 | 177203 | 179506 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 92.97 | 99.22 | AAK74619 |
| GGEMHDLO_23 | 24846 | 25814 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| GGEMHDLO_41 | 29865 | 31346 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.11 | 100.00 | ABJ54480 |
| GGEMHDLO_8 | 27616 | 25370 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| GGEMHDLO_7 | 36970 | 37968 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| GGEMHDLO_7 | 1 | 957 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.60 | 98.46 | ABF36968 |
| GGEMHDLO_1 | 63279 | 62128 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| GGEMHDLO_1 | 35279 | 33291 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| GGEMHDLO_12 | 39156 | 40886 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| GGEMHDLO_40 | 56189 | 54711 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| GGEMHDLO_23 | 106041 | 107342 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| GGEMHDLO_40 | 40993 | 39101 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| GGEMHDLO_8 | 22445 | 24154 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| GGEMHDLO_39 | 56673 | 55663 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| GGEMHDLO_34 | 64534 | 65895 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| GGEMHDLO_23 | 91602 | 92492 | bglB | 4.78e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| GGEMHDLO_4 | 40762 | 41697 | SP_0676 | 2.06e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| GGEMHDLO_8 | 25178 | 24321 | metF | 1.37e-165 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| GGEMHDLO_12 | 138258 | 138962 | covR | 9.34e-132 | responder protein | 85.11 | 100.00 | AAK71319 |
| GGEMHDLO_41 | 5279 | 4608 | rr01 | 4.29e-124 | response regulator | 83.04 | 99.56 | CAB54566 |
| GGEMHDLO_12 | 138258 | 138962 | walR | 4.68e-122 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| GGEMHDLO_8 | 18491 | 17823 | CiaR | 1.25e-121 | two-component response regulator | 85.65 | 99.55 | AAK74935 |
| GGEMHDLO_8 | 18491 | 17820 | ciaR | 1.52e-119 | two component system response regulator | 83.48 | 99.12 | AIK75708 |
| GGEMHDLO_37 | 83635 | 84321 | CovR | 5.06e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| GGEMHDLO_7 | 38244 | 38618 | SpxA2 | 2.41e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| GGEMHDLO_34 | 53175 | 52774 | SpxA1 | 2.10e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| GGEMHDLO_6 | 25341 | 25054 | spr0479 | 3.06e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| GGEMHDLO_38 | 81360 | 81614 | spr1327 | 1.36e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| GGEMHDLO_40 | 7370 | 7633 | spr0175 | 4.00e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| GGEMHDLO_40 | 8197 | 8463 | spr0177 | 7.06e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| GGEMHDLO_2 | 3488 | 3988 | RopE (M5005_Spy_1611) | 1.80e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| GGEMHDLO_1 | 177203 | 179500 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.81 | 98.08 | MYN69560 |
| GGEMHDLO_39 | 19128 | 20147 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.18 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| GGEMHDLO_9 | 88 | 1035 | rep37 | 950 / 948 | 95.26 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GGEMHDLO_1 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_2 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_3 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_4 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_5 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_6 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_7 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_8 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_9 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_10 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_11 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_12 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_13 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_14 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_15 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_16 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_17 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_18 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_19 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_21 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_22 | 1.0 | 0.0 | 1.0 |
| GGEMHDLO_23 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_24 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_25 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_26 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_27 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_28 | 1.0 | 0.0 | 1.0 |
| GGEMHDLO_29 | 1.0 | 0.0 | 1.0 |
| GGEMHDLO_30 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_31 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_32 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_33 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_34 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_37 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_38 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_39 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_40 | 0.0 | 1.0 | 0.0 |
| GGEMHDLO_41 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)