Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 25 | 32 | 40.61 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DOCEAPPB_27 | 9088 | 9927 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| DOCEAPPB_1 | 270756 | 271811 | STU_RS16235 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_002948022 |
| DOCEAPPB_1 | 268722 | 269531 | STU_RS16245 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_002948020 |
| DOCEAPPB_1 | 271808 | 273592 | STU_RS16230 | 0.0 | Capsule | Immune modulation | 99.94 | 100.00 | WP_011226325 |
| DOCEAPPB_1 | 269531 | 270736 | STU_RS16240 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226326 |
| DOCEAPPB_1 | 267782 | 268738 | STU_RS16250 | 0.0 | Capsule | Immune modulation | 99.79 | 100.00 | WP_011226328 |
| DOCEAPPB_23 | 39710 | 40303 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | WP_011226149 |
| DOCEAPPB_1 | 266637 | 267785 | STU_RS16255 | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | WP_041828290 |
| DOCEAPPB_23 | 39710 | 40303 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| DOCEAPPB_25 | 9224 | 9823 | STR_RS05245 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_196767671 |
| DOCEAPPB_23 | 39710 | 40303 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011226149 |
| DOCEAPPB_10 | 488 | 2146 | fbp54 | 0.0 | FBPs | Adherence | 99.46 | 100.00 | WP_011227179 |
| DOCEAPPB_44 | 1 | 1369 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 99.42 | 94.09 | WP_041827043 |
| DOCEAPPB_23 | 84339 | 85129 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 99.11 | 100.00 | - |
| DOCEAPPB_23 | 38841 | 39710 | rfbA | 0.0 | Capsule | Immune modulation | 99.08 | 100.00 | WP_011681286 |
| DOCEAPPB_25 | 9974 | 10709 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 99.05 | 99.33 | WP_011681174 |
| DOCEAPPB_23 | 4181 | 4942 | srtA | 0.0 | Sortase A | Exoenzyme | 98.95 | 100.00 | - |
| DOCEAPPB_25 | 9960 | 10709 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.93 | 100.00 | WP_011227246 |
| DOCEAPPB_1 | 262704 | 263978 | STU_RS16265 | 0.0 | Capsule | Immune modulation | 98.82 | 100.00 | WP_011226331 |
| DOCEAPPB_1 | 263978 | 264940 | STU_RS16260 | 0.0 | Capsule | Immune modulation | 98.75 | 95.25 | WP_011226330 |
| DOCEAPPB_25 | 9974 | 10709 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.37 | 99.33 | WP_011226046 |
| DOCEAPPB_44 | 1 | 1369 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 98.25 | 94.09 | WP_011226049 |
| DOCEAPPB_25 | 8100 | 9059 | STR_RS05240 | 0.0 | Capsule | Immune modulation | 98.23 | 100.00 | WP_041827042 |
| DOCEAPPB_25 | 10719 | 11411 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011227247 |
| DOCEAPPB_25 | 10719 | 11411 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 97.69 | 100.00 | WP_011226047 |
| DOCEAPPB_44 | 1 | 1369 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.16 | 93.91 | WP_011681177 |
| DOCEAPPB_1 | 260752 | 261603 | rfbD | 0.0 | Capsule | Immune modulation | 96.24 | 100.00 | WP_014621820 |
| DOCEAPPB_25 | 10719 | 11411 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 90.91 | 100.00 | WP_011681175 |
| DOCEAPPB_9 | 692 | 1995 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.10 | 99.46 | WP_000022832 |
| DOCEAPPB_19 | 215 | 1225 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.82 | 99.51 | WP_002986042 |
| DOCEAPPB_56 | 64809 | 66092 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| DOCEAPPB_23 | 40732 | 41778 | rfbB | 0.0 | Capsule | Immune modulation | 80.33 | 100.00 | WP_009659423 |
| DOCEAPPB_35 | 2213 | 2796 | SPD_RS01775 | 2.83e-149 | Capsule | Immune modulation | 83.08 | 96.20 | WP_001819836 |
| DOCEAPPB_44 | 1370 | 1812 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 99.10 | 60.52 | WP_011227248 |
| DOCEAPPB_44 | 1370 | 1812 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.97 | 60.52 | WP_011226048 |
| DOCEAPPB_44 | 1370 | 1812 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 97.74 | 60.52 | WP_011681176 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DOCEAPPB_23 | 1494 | 511 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| DOCEAPPB_53 | 13749 | 12676 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| DOCEAPPB_1 | 105621 | 103318 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| DOCEAPPB_8 | 24783 | 25751 | spr0084 | 0.0 | pathogenitcity related | 92.88 | 98.48 | AAK98888 |
| DOCEAPPB_34 | 29866 | 31347 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.90 | 100.00 | ABJ54480 |
| DOCEAPPB_16 | 15245 | 12999 | metE | 0.0 | methionine biosynthesis enzymes | 91.72 | 100.00 | AAK74738 |
| DOCEAPPB_21 | 1872 | 874 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| DOCEAPPB_20 | 1 | 957 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.60 | 98.46 | ABF36968 |
| DOCEAPPB_1 | 219545 | 220696 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| DOCEAPPB_1 | 247545 | 249533 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.84 | 100.00 | AE014259_2 |
| DOCEAPPB_23 | 17690 | 19420 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| DOCEAPPB_3 | 56188 | 54710 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| DOCEAPPB_9 | 692 | 1993 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| DOCEAPPB_3 | 40993 | 39101 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| DOCEAPPB_16 | 10074 | 11783 | PGM | 0.0 | phosphoglucomutase | 83.89 | 99.65 | ACH87041 |
| DOCEAPPB_12 | 80479 | 79469 | pdh | 0.0 | pyruvate dehydrogenase | 83.38 | 99.70 | ABP91006 |
| DOCEAPPB_10 | 26520 | 25159 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.24 | 99.56 | ABP90420 |
| DOCEAPPB_54 | 13732 | 12842 | bglB | 2.55e-179 | putative phospho--glucosidase | 88.55 | 99.66 | AAK33487 |
| DOCEAPPB_5 | 40866 | 41801 | SP_0676 | 2.00e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| DOCEAPPB_16 | 12807 | 11950 | metF | 8.56e-166 | methionine biosynthesis enzymes | 86.01 | 99.31 | AAK74739 |
| DOCEAPPB_7 | 17331 | 18035 | covR | 1.99e-132 | responder protein | 85.11 | 100.00 | AAK71319 |
| DOCEAPPB_34 | 5280 | 4609 | rr01 | 5.27e-124 | response regulator | 83.04 | 99.56 | CAB54566 |
| DOCEAPPB_7 | 17331 | 18035 | walR | 9.97e-123 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| DOCEAPPB_16 | 6120 | 5452 | CiaR | 7.82e-122 | two-component response regulator | 85.65 | 99.55 | AAK74935 |
| DOCEAPPB_16 | 6120 | 5449 | ciaR | 9.52e-120 | two component system response regulator | 83.48 | 99.12 | AIK75708 |
| DOCEAPPB_45 | 83938 | 84624 | CovR | 5.07e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| DOCEAPPB_21 | 598 | 224 | SpxA2 | 7.44e-72 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| DOCEAPPB_2 | 53238 | 52837 | SpxA1 | 1.30e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| DOCEAPPB_6 | 14758 | 15045 | spr0479 | 3.06e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| DOCEAPPB_55 | 60600 | 60854 | spr1327 | 1.05e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| DOCEAPPB_3 | 7370 | 7633 | spr0175 | 2.85e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| DOCEAPPB_3 | 8197 | 8463 | spr0177 | 5.03e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| DOCEAPPB_56 | 64651 | 64151 | RopE (M5005_Spy_1611) | 1.80e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| DOCEAPPB_1 | 105621 | 103324 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 82.94 | 98.08 | MYN69560 |
| DOCEAPPB_12 | 42934 | 43953 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.18 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| DOCEAPPB_37 | 240 | 1187 | rep37 | 950 / 948 | 95.26 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DOCEAPPB_1 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_2 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_3 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_4 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_5 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_6 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_7 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_8 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_9 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_10 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_11 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_12 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_13 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_14 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_15 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_16 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_17 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_18 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_19 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_20 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_21 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_22 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_23 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_24 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_25 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_26 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_27 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_28 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_29 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_30 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_31 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_32 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_33 | 1.0 | 0.0 | 1.0 |
| DOCEAPPB_34 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_35 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_36 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_37 | 1.0 | 0.0 | 1.0 |
| DOCEAPPB_38 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_39 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_40 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_41 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_42 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_43 | 1.0 | 0.0 | 1.0 |
| DOCEAPPB_44 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_45 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_46 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_53 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_54 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_55 | 0.0 | 1.0 | 0.0 |
| DOCEAPPB_56 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)