Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 33 | 40.22 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OEDCMFPA_16 | 128 | 967 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| OEDCMFPA_12 | 115125 | 116384 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| OEDCMFPA_3 | 60793 | 61386 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| OEDCMFPA_3 | 60793 | 61386 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| OEDCMFPA_3 | 60793 | 61386 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| OEDCMFPA_3 | 59924 | 60793 | rfbA | 0.0 | Capsule | Immune modulation | 99.54 | 100.00 | WP_011681286 |
| OEDCMFPA_1 | 74675 | 76333 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| OEDCMFPA_12 | 116381 | 117357 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.39 | 99.90 | WP_011681444 |
| OEDCMFPA_12 | 117375 | 118343 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | WP_011681443 |
| OEDCMFPA_5 | 28249 | 28698 | STU_RS14620 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226042 |
| OEDCMFPA_5 | 31368 | 32108 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 99.19 | 100.00 | WP_011681174 |
| OEDCMFPA_3 | 25266 | 26027 | srtA | 0.0 | Sortase A | Exoenzyme | 99.08 | 100.00 | - |
| OEDCMFPA_45 | 1365 | 2155 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 100.00 | - |
| OEDCMFPA_5 | 31368 | 32108 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_011226046 |
| OEDCMFPA_5 | 28707 | 29703 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 98.39 | 100.00 | - |
| OEDCMFPA_5 | 31373 | 32108 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.37 | 98.13 | WP_011227246 |
| OEDCMFPA_12 | 118345 | 119060 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.32 | 99.86 | WP_011681442 |
| OEDCMFPA_5 | 33551 | 35011 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.81 | 100.00 | WP_011681177 |
| OEDCMFPA_12 | 119406 | 120176 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.80 | 99.23 | WP_011681440 |
| OEDCMFPA_12 | 114155 | 115006 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| OEDCMFPA_5 | 32819 | 33550 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.13 | 100.00 | WP_011226048 |
| OEDCMFPA_5 | 33551 | 35011 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.06 | 100.21 | WP_011226049 |
| OEDCMFPA_5 | 32819 | 33550 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 96.45 | 100.00 | WP_011227248 |
| OEDCMFPA_5 | 32819 | 33550 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 96.17 | 100.00 | WP_011681176 |
| OEDCMFPA_5 | 33551 | 35011 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.10 | 100.21 | WP_041827043 |
| OEDCMFPA_5 | 32118 | 32810 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_011226047 |
| OEDCMFPA_5 | 32118 | 32810 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 94.66 | 100.00 | WP_011227247 |
| OEDCMFPA_5 | 29947 | 31312 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.51 | 99.85 | WP_011681173 |
| OEDCMFPA_5 | 29945 | 31312 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.01 | 100.00 | WP_011226045 |
| OEDCMFPA_5 | 32118 | 32810 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 92.64 | 100.00 | WP_011681175 |
| OEDCMFPA_23 | 28146 | 29449 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.02 | 99.46 | WP_000022832 |
| OEDCMFPA_25 | 136 | 1146 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| OEDCMFPA_2 | 2265 | 3548 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| OEDCMFPA_3 | 61816 | 62862 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
| OEDCMFPA_5 | 27942 | 28249 | STU_RS14615 | 6.09e-135 | Capsule | Immune modulation | 95.13 | 63.37 | WP_011226041 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OEDCMFPA_3 | 22579 | 21596 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| OEDCMFPA_63 | 14760 | 13687 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| OEDCMFPA_34 | 40845 | 43148 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.23 | 99.22 | AAK74619 |
| OEDCMFPA_23 | 111752 | 110784 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| OEDCMFPA_64 | 56784 | 58265 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| OEDCMFPA_8 | 6271 | 8517 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| OEDCMFPA_26 | 15240 | 14284 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.60 | 98.46 | ABF36968 |
| OEDCMFPA_12 | 73113 | 74264 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| OEDCMFPA_12 | 101118 | 103106 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| OEDCMFPA_3 | 38774 | 40504 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| OEDCMFPA_56 | 85361 | 83883 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| OEDCMFPA_23 | 29449 | 28148 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| OEDCMFPA_56 | 70669 | 68777 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| OEDCMFPA_16 | 19760 | 20866 | recA | 0.0 | DNA recombination and repair | 84.91 | 95.36 | AAL00560 |
| OEDCMFPA_8 | 11442 | 9733 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| OEDCMFPA_65 | 19039 | 20049 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| OEDCMFPA_1 | 100240 | 98879 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| OEDCMFPA_65 | 69333 | 67441 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| OEDCMFPA_23 | 43888 | 42998 | bglB | 9.77e-180 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| OEDCMFPA_1 | 11782 | 10847 | SP_0676 | 6.06e-169 | transcription facot | 81.73 | 96.89 | AAK74821 |
| OEDCMFPA_8 | 8709 | 9566 | metF | 1.63e-166 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| OEDCMFPA_45 | 69761 | 70465 | covR | 5.41e-132 | responder protein | 85.11 | 100.00 | AAK71319 |
| OEDCMFPA_64 | 28683 | 28012 | rr01 | 1.84e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| OEDCMFPA_8 | 15395 | 16063 | CiaR | 1.59e-122 | two-component response regulator | 86.10 | 99.55 | AAK74935 |
| OEDCMFPA_45 | 69761 | 70465 | walR | 2.71e-122 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| OEDCMFPA_8 | 15395 | 16066 | ciaR | 1.82e-120 | two component system response regulator | 83.93 | 99.12 | AIK75708 |
| OEDCMFPA_11 | 16116 | 16802 | CovR | 1.39e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| OEDCMFPA_16 | 21049 | 21423 | SpxA2 | 1.34e-71 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| OEDCMFPA_1 | 111624 | 112025 | SpxA1 | 3.09e-67 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| OEDCMFPA_17 | 14760 | 15047 | spr0479 | 1.59e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| OEDCMFPA_14 | 9885 | 9631 | spr1327 | 3.77e-46 | essential gene | 95.29 | 100.00 | AAL00131 |
| OEDCMFPA_56 | 37040 | 37303 | spr0175 | 4.40e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| OEDCMFPA_56 | 37867 | 38133 | spr0177 | 7.77e-34 | essential gene | 84.27 | 88.12 | AAK98981 |
| OEDCMFPA_2 | 3706 | 4206 | RopE (M5005_Spy_1611) | 1.75e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| OEDCMFPA_34 | 40845 | 43142 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| OEDCMFPA_65 | 54904 | 53885 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| OEDCMFPA_35 | 1 | 853 | rep37 | 855 / 948 | 94.85 | AF177168 |
| OEDCMFPA_40 | 1060 | 1727 | rep13 | 674 / 678 | 89.17 | V01277 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OEDCMFPA_1 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_2 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_3 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_4 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_5 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_6 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_7 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_8 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_9 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_10 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_11 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_12 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_13 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_14 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_15 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_16 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_17 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_18 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_19 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_20 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_21 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_22 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_23 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_24 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_25 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_26 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_27 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_28 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_29 | 1.0 | 0.0 | 1.0 |
| OEDCMFPA_30 | 1.0 | 0.0 | 1.0 |
| OEDCMFPA_31 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_32 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_33 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_34 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_35 | 1.0 | 0.0 | 1.0 |
| OEDCMFPA_36 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_37 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_38 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_39 | 1.0 | 0.0 | 1.0 |
| OEDCMFPA_40 | 1.0 | 0.0 | 1.0 |
| OEDCMFPA_41 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_42 | 1.0 | 0.0 | 1.0 |
| OEDCMFPA_43 | 1.0 | 0.0 | 1.0 |
| OEDCMFPA_44 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_45 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_46 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_47 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_48 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_49 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_50 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_56 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_63 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_64 | 0.0 | 1.0 | 0.0 |
| OEDCMFPA_65 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OEDCMFPA_45 | 13396 | 55762 | False | Siphoviridae | VOG0275, VOG4040, VOG4602, VOG6127, VOG3622, VOG4552, VOG0650, VOG0651, VOG0321, VOG6420, VOG6419, VOG0322, VOG6545, VOG4632, VOG4609, VOG0655, VOG3468, VOG0656, VOG6414, VOG6140, VOG0152, VOG6495, VOG0657, VOG0658, VOG0198, VOG4841, VOG4581, VOG1886, VOG0202, VOG4556, VOG4573, VOG4553, VOG0204, VOG0205, VOG0207, VOG4586, VOG0209, VOG6163, VOG4605, VOG4599, VOG0647, VOG4782, VOG3298, VOG0641, VOG0054, VOG4649, VOG3299, VOG3300, VOG5284, VOG4134 |
Phigaro v2.4.0 (Database: Jan. 2024 release)