Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 15 | 34 | 37.16 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KPMDMHFH_22 | 51152 | 51892 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 95.01 | 100.00 | WP_011681174 |
| KPMDMHFH_22 | 51152 | 51892 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 94.33 | 100.00 | WP_011226046 |
| KPMDMHFH_22 | 48249 | 49709 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.11 | 100.14 | WP_011226049 |
| KPMDMHFH_22 | 48249 | 49709 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 93.98 | 100.00 | WP_011681177 |
| KPMDMHFH_22 | 51949 | 53316 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.93 | 100.00 | WP_011226045 |
| KPMDMHFH_19 | 74136 | 74987 | rfbD | 0.0 | Capsule | Immune modulation | 93.90 | 100.00 | WP_014621820 |
| KPMDMHFH_22 | 51152 | 51887 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.75 | 98.13 | WP_011227246 |
| KPMDMHFH_22 | 51949 | 53314 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.63 | 99.85 | WP_011681173 |
| KPMDMHFH_22 | 48249 | 49709 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.22 | 100.14 | WP_041827043 |
| KPMDMHFH_22 | 49710 | 50441 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 91.94 | 100.00 | WP_011226048 |
| KPMDMHFH_22 | 49710 | 50441 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 91.53 | 100.00 | WP_011681176 |
| KPMDMHFH_11 | 457957 | 458826 | rfbA | 0.0 | Capsule | Immune modulation | 91.38 | 100.00 | WP_011681286 |
| KPMDMHFH_22 | 158283 | 159941 | fbp54 | 0.0 | FBPs | Adherence | 91.15 | 99.88 | WP_011227179 |
| KPMDMHFH_22 | 49710 | 50441 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 90.98 | 100.00 | WP_011227248 |
| KPMDMHFH_11 | 458826 | 459419 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 90.74 | 100.00 | WP_011226149 |
| KPMDMHFH_11 | 458826 | 459419 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 90.57 | 100.00 | WP_011226149 |
| KPMDMHFH_11 | 458826 | 459419 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 90.57 | 100.00 | WP_011226149 |
| KPMDMHFH_19 | 382093 | 383103 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.45 | 99.80 | WP_002986042 |
| KPMDMHFH_11 | 276650 | 277953 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.24 | 99.54 | WP_000022832 |
| KPMDMHFH_11 | 90930 | 91829 | cbpD | 0.0 | Autolysin | Exoenzyme | 88.08 | 93.40 | WP_082302295 |
| KPMDMHFH_22 | 50450 | 51142 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.04 | 99.86 | WP_011227247 |
| KPMDMHFH_22 | 50450 | 51142 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.04 | 99.86 | WP_011226047 |
| KPMDMHFH_22 | 50450 | 51142 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.46 | 99.86 | WP_011681175 |
| KPMDMHFH_23 | 55426 | 56187 | srtA | 0.0 | Sortase A | Exoenzyme | 84.67 | 99.87 | - |
| KPMDMHFH_21 | 218184 | 219467 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.61 | 99.61 | WP_000107753 |
| KPMDMHFH_11 | 459623 | 460669 | rfbB | 0.0 | Capsule | Immune modulation | 80.33 | 100.00 | WP_009659423 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KPMDMHFH_23 | 64320 | 65393 | MntE | 0.0 | manganese homeostasis | 96.09 | 99.72 | ABF32167 |
| KPMDMHFH_23 | 52737 | 51754 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.04 | 100.00 | AAK75326 |
| KPMDMHFH_19 | 256301 | 258607 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.41 | 99.22 | AAK74619 |
| KPMDMHFH_24 | 61230 | 59749 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| KPMDMHFH_23 | 83380 | 85626 | metE | 0.0 | methionine biosynthesis enzymes | 92.52 | 100.00 | AAK74738 |
| KPMDMHFH_11 | 185982 | 186950 | spr0084 | 0.0 | pathogenitcity related | 92.26 | 98.48 | AAK98888 |
| KPMDMHFH_3 | 23662 | 22691 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| KPMDMHFH_26 | 7046 | 483 | PrtA | 0.0 | serine protease | 90.05 | 101.96 | ABJ55268 |
| KPMDMHFH_11 | 432570 | 434300 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.95 | 100.00 | AAK34199 |
| KPMDMHFH_19 | 116932 | 115781 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.58 | 99.74 | AAL00050 |
| KPMDMHFH_19 | 88526 | 86538 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.44 | 100.00 | AE014259_2 |
| KPMDMHFH_20 | 218531 | 217053 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.83 | 100.00 | ADE32434 |
| KPMDMHFH_11 | 276650 | 277951 | Eno | 0.0 | enolase | 86.44 | 99.77 | ABP90468 |
| KPMDMHFH_20 | 203173 | 201281 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.58 | 98.90 | ABP91129 |
| KPMDMHFH_23 | 88545 | 86836 | PGM | 0.0 | phosphoglucomutase | 84.76 | 99.65 | ACH87041 |
| KPMDMHFH_8 | 11800 | 12891 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| KPMDMHFH_20 | 28541 | 29551 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| KPMDMHFH_22 | 144515 | 145876 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| KPMDMHFH_11 | 262241 | 263131 | bglB | 2.74e-177 | putative phospho--glucosidase | 87.54 | 99.66 | AAK33487 |
| KPMDMHFH_23 | 129236 | 128286 | SP_0676 | 2.18e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| KPMDMHFH_23 | 85813 | 86670 | metF | 4.43e-169 | methionine biosynthesis enzymes | 87.76 | 99.31 | AAK74739 |
| KPMDMHFH_26 | 30624 | 29953 | rr01 | 7.39e-128 | response regulator | 84.82 | 99.56 | CAB54566 |
| KPMDMHFH_22 | 2575 | 3279 | covR | 8.11e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| KPMDMHFH_23 | 92512 | 93183 | ciaR | 1.68e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| KPMDMHFH_23 | 92512 | 93180 | CiaR | 3.11e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| KPMDMHFH_22 | 2575 | 3279 | walR | 1.84e-120 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| KPMDMHFH_21 | 53882 | 53196 | CovR | 1.65e-119 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| KPMDMHFH_8 | 13064 | 13459 | SpxA2 | 1.35e-76 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| KPMDMHFH_22 | 133034 | 132633 | SpxA1 | 3.73e-70 | transcriptional regulator | 82.84 | 100.00 | ERL19273 |
| KPMDMHFH_1 | 31119 | 31532 | GrpE | 2.91e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| KPMDMHFH_21 | 30674 | 30387 | spr0479 | 4.68e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| KPMDMHFH_11 | 147202 | 147456 | spr1327 | 2.17e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| KPMDMHFH_20 | 150373 | 150636 | spr0175 | 1.26e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| KPMDMHFH_20 | 151197 | 151463 | spr0177 | 2.81e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| KPMDMHFH_21 | 218017 | 217526 | RopE (M5005_Spy_1611) | 1.07e-72 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| KPMDMHFH_19 | 256301 | 258601 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.09 | 98.08 | MYN69560 |
| KPMDMHFH_20 | 64978 | 63959 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KPMDMHFH_1 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_2 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_3 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_4 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_5 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_6 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_7 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_8 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_9 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_10 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_11 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_12 | 1.0 | 0.0 | 1.0 |
| KPMDMHFH_14 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_15 | 1.0 | 0.0 | 1.0 |
| KPMDMHFH_16 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_19 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_20 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_21 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_22 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_23 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_24 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_25 | 0.0 | 1.0 | 0.0 |
| KPMDMHFH_26 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)