Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 15 | 35 | 38.13 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| GKEIBBBJ_11 | 271816 | 273279 | msr(D) | Erythromycin, Azithromycin, Telithromycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 100.00 | 100.00 | AF274302 |
| GKEIBBBJ_11 | 273399 | 274616 | mef(A) | Erythromycin, Azithromycin | 99.92 | 100.00 | U83667 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| GKEIBBBJ_11 | 273402 | 274616 | mef(A) | macrolide efflux MFS transporter Mef(A) | EXACTX | 100.00 | 100.00 | WP_000417519.1 |
| GKEIBBBJ_11 | 271819 | 273279 | msr(D) | ABC-F type ribosomal protection protein Msr(D) | EXACTX | 100.00 | 100.00 | WP_000420313.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| GKEIBBBJ_22 | 17468 | 18208 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 94.33 | 100.00 | WP_011681174 |
| GKEIBBBJ_22 | 14565 | 16025 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 94.18 | 100.14 | WP_011226049 |
| GKEIBBBJ_11 | 121496 | 122089 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 94.11 | 100.00 | WP_011226149 |
| GKEIBBBJ_22 | 14565 | 16025 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 94.05 | 100.00 | WP_011681177 |
| GKEIBBBJ_11 | 121496 | 122089 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 93.94 | 100.00 | WP_011226149 |
| GKEIBBBJ_11 | 121496 | 122089 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 93.94 | 100.00 | WP_011226149 |
| GKEIBBBJ_22 | 17468 | 18208 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 93.93 | 100.00 | WP_011226046 |
| GKEIBBBJ_22 | 18265 | 19630 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 93.78 | 99.85 | WP_011681173 |
| GKEIBBBJ_22 | 18265 | 19632 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 93.49 | 100.00 | WP_011226045 |
| GKEIBBBJ_22 | 17468 | 18203 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 93.34 | 98.13 | WP_011227246 |
| GKEIBBBJ_22 | 14565 | 16025 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 93.29 | 100.14 | WP_041827043 |
| GKEIBBBJ_22 | 16026 | 16757 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 92.62 | 100.00 | WP_011226048 |
| GKEIBBBJ_22 | 16026 | 16757 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 92.49 | 100.00 | WP_011681176 |
| GKEIBBBJ_22 | 16026 | 16757 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 91.67 | 100.00 | WP_011227248 |
| GKEIBBBJ_34 | 45660 | 46510 | rfbD | 0.0 | Capsule | Immune modulation | 91.66 | 99.88 | WP_014621820 |
| GKEIBBBJ_11 | 122089 | 122958 | rfbA | 0.0 | Capsule | Immune modulation | 91.38 | 100.00 | WP_011681286 |
| GKEIBBBJ_22 | 133142 | 134799 | fbp54 | 0.0 | FBPs | Adherence | 90.23 | 99.88 | WP_011227179 |
| GKEIBBBJ_12 | 14386 | 15396 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 88.55 | 99.80 | WP_002986042 |
| GKEIBBBJ_22 | 16766 | 17458 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 88.18 | 99.86 | WP_011227247 |
| GKEIBBBJ_22 | 16766 | 17458 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 88.18 | 99.86 | WP_011226047 |
| GKEIBBBJ_11 | 333390 | 334693 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| GKEIBBBJ_10 | 3649 | 4554 | cbpD | 0.0 | Autolysin | Exoenzyme | 87.86 | 93.50 | WP_082302295 |
| GKEIBBBJ_22 | 16766 | 17458 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 87.61 | 99.86 | WP_011681175 |
| GKEIBBBJ_11 | 120178 | 121224 | rfbB | 0.0 | Capsule | Immune modulation | 84.45 | 99.90 | WP_002263087 |
| GKEIBBBJ_37 | 32393 | 33154 | srtA | 0.0 | Sortase A | Exoenzyme | 83.64 | 99.74 | - |
| GKEIBBBJ_17 | 7449 | 8732 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.92 | 99.61 | WP_000107753 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| GKEIBBBJ_37 | 29704 | 28730 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 96.31 | 99.09 | AAK75326 |
| GKEIBBBJ_37 | 41289 | 42362 | MntE | 0.0 | manganese homeostasis | 95.25 | 99.72 | ABF32167 |
| GKEIBBBJ_2 | 44031 | 46337 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 94.54 | 99.22 | AAK74619 |
| GKEIBBBJ_11 | 422466 | 421498 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| GKEIBBBJ_36 | 82462 | 83943 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 93.12 | 100.00 | ABJ54480 |
| GKEIBBBJ_37 | 60355 | 62601 | metE | 0.0 | methionine biosynthesis enzymes | 92.12 | 100.00 | AAK74738 |
| GKEIBBBJ_6 | 34496 | 33525 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 91.67 | 100.00 | ABF36968 |
| GKEIBBBJ_34 | 3758 | 4906 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 90.08 | 99.48 | AAL00050 |
| GKEIBBBJ_36 | 7046 | 483 | PrtA | 0.0 | serine protease | 90.05 | 101.96 | ABJ55268 |
| GKEIBBBJ_11 | 153856 | 152126 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 89.78 | 100.00 | AAK34199 |
| GKEIBBBJ_34 | 31994 | 33982 | uvrB | 0.0 | excinuclease ABC, B subunit | 89.59 | 100.00 | AE014259_2 |
| GKEIBBBJ_32 | 26001 | 27479 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| GKEIBBBJ_11 | 334693 | 333392 | Eno | 0.0 | enolase | 85.98 | 99.77 | ABP90468 |
| GKEIBBBJ_32 | 41316 | 43208 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.26 | 98.90 | ABP91129 |
| GKEIBBBJ_37 | 65519 | 63810 | PGM | 0.0 | phosphoglucomutase | 84.94 | 99.65 | ACH87041 |
| GKEIBBBJ_19 | 4221 | 5312 | recA | 0.0 | DNA recombination and repair | 84.64 | 95.36 | AAL00560 |
| GKEIBBBJ_32 | 174383 | 173373 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| GKEIBBBJ_22 | 106880 | 108241 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.67 | 99.56 | ABP90420 |
| GKEIBBBJ_11 | 349099 | 348209 | bglB | 6.34e-178 | putative phospho--glucosidase | 87.88 | 99.66 | AAK33487 |
| GKEIBBBJ_22 | 193568 | 194518 | SP_0676 | 4.61e-170 | transcription facot | 81.39 | 98.45 | AAK74821 |
| GKEIBBBJ_37 | 62787 | 63644 | metF | 4.38e-169 | methionine biosynthesis enzymes | 87.06 | 99.31 | AAK74739 |
| GKEIBBBJ_34 | 45660 | 46499 | GacA | 1.91e-151 | dTDP-4-dehydrorhamnose reductas | 80.71 | 98.59 | AAZ51220 |
| GKEIBBBJ_8 | 31070 | 30366 | covR | 1.45e-127 | responder protein | 85.53 | 100.00 | AAK71319 |
| GKEIBBBJ_36 | 25155 | 24484 | rr01 | 2.47e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| GKEIBBBJ_37 | 69486 | 70157 | ciaR | 1.27e-125 | two component system response regulator | 86.16 | 99.12 | AIK75708 |
| GKEIBBBJ_37 | 69486 | 70154 | CiaR | 2.33e-125 | two-component response regulator | 87.44 | 99.55 | AAK74935 |
| GKEIBBBJ_8 | 31070 | 30366 | walR | 3.29e-121 | DNA-binding response regulator | 82.13 | 100.00 | ANC99504 |
| GKEIBBBJ_33 | 53917 | 53231 | CovR | 4.42e-120 | serves to repress GAS virulence factor-encoding genes | 81.66 | 100.00 | ACE75886 |
| GKEIBBBJ_19 | 5488 | 5883 | SpxA2 | 5.99e-77 | transcriptional regulator | 91.67 | 100.00 | ERL21461 |
| GKEIBBBJ_22 | 95412 | 95011 | SpxA1 | 3.20e-69 | transcriptional regulator | 82.09 | 100.00 | ERL19273 |
| GKEIBBBJ_15 | 6239 | 5826 | GrpE | 1.24e-68 | protein grpE | 81.88 | 80.59 | ABP89267 |
| GKEIBBBJ_33 | 30710 | 30423 | spr0479 | 4.28e-48 | essential gene | 85.42 | 98.97 | AAK99283 |
| GKEIBBBJ_11 | 455188 | 454934 | spr1327 | 2.21e-44 | essential gene | 92.94 | 100.00 | AAL00131 |
| GKEIBBBJ_32 | 82709 | 82446 | spr0175 | 1.05e-42 | pathogenitcity related | 85.23 | 100.00 | AAK98979 |
| GKEIBBBJ_32 | 81883 | 81617 | spr0177 | 1.83e-41 | essential gene | 83.15 | 88.12 | AAK98981 |
| GKEIBBBJ_17 | 7282 | 6791 | RopE (M5005_Spy_1611) | 5.31e-74 | probable DNA-directed RNA polymerase subunit delta | 82.93 | 85.86 | AAK34603 |
| GKEIBBBJ_2 | 44031 | 46331 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.22 | 98.08 | MYN69560 |
| GKEIBBBJ_32 | 137211 | 138230 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 97.35 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| GKEIBBBJ_1 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_2 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_3 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_4 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_5 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_6 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_7 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_8 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_9 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_10 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_11 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_12 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_13 | 1.0 | 0.0 | 1.0 |
| GKEIBBBJ_14 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_15 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_16 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_17 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_18 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_19 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_22 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_23 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_25 | 1.0 | 0.0 | 1.0 |
| GKEIBBBJ_28 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_29 | 1.0 | 0.0 | 1.0 |
| GKEIBBBJ_31 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_32 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_33 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_34 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_35 | 1.0 | 0.0 | 1.0 |
| GKEIBBBJ_36 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_37 | 0.0 | 1.0 | 0.0 |
| GKEIBBBJ_38 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| GKEIBBBJ_11 | 294738 | 331647 | False | Siphoviridae | VOG8294, VOG0054, VOG0641, VOG5008, VOG4828, VOG4633, VOG3636, VOG0701, VOG8357, VOG0698, VOG0697, VOG0696, VOG0695, VOG4572, VOG4555, VOG0692, VOG0691, VOG4544, VOG0796, VOG4987, VOG6140, VOG0657, VOG0152, VOG0656, VOG0655, VOG7265, VOG4693, VOG7264, VOG4548, VOG7263, VOG5317, VOG5280, VOG0322, VOG0083, VOG0651, VOG3478, VOG3547, VOG6495 |
Phigaro v2.4.0 (Database: Jan. 2024 release)