Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 0 | 1 | 4.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LEMJHGCK_33 | 229372 | 230196 | ErmF | ARO:3000498 | ErmF | 97.62 | 103.01 | M17124.1 |
| LEMJHGCK_7 | 92523 | 94448 | tet(Q) | ARO:3000191 | tet(Q) | 96.88 | 97.56 | Z21523.1 |
| LEMJHGCK_15 | 66910 | 67875 | CfxA2 | ARO:3003002 | CfxA2 | 99.69 | 100.00 | AF118110.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LEMJHGCK_33 | 229372 | 230128 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.87 | 94.51 | M62487 |
| LEMJHGCK_15 | 66910 | 67875 | cfxA3 | Ampicillin | 99.79 | 100.00 | AF472622 |
| LEMJHGCK_15 | 66910 | 67875 | cfxA4 | Unknown Beta-lactam | 99.79 | 100.00 | AY769933 |
| LEMJHGCK_15 | 66910 | 67875 | cfxA5 | Unknown Beta-lactam | 99.79 | 100.00 | AY769934 |
| LEMJHGCK_15 | 66910 | 67875 | cfxA | Cefoxitin, Ampicillin | 99.79 | 100.00 | U38243 |
| LEMJHGCK_7 | 92523 | 94448 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 96.68 | 100.00 | Z21523 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LEMJHGCK_48 | 254 | 1159 | blaMUN-1 | MUN family extended-spectrum class A beta-lactamase MUN-1 | ALLELEX | 100.00 | 100.00 | WP_206340447.1 |
| LEMJHGCK_15 | 66913 | 67875 | cfxA | CfxA family broad-spectrum class A beta-lactamase | BLASTX | 99.69 | 100.00 | WP_004339683.1 |
| LEMJHGCK_33 | 229372 | 230127 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 99.60 | 94.74 | WP_063844771.1 |
| LEMJHGCK_7 | 92523 | 94445 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | BLASTX | 96.88 | 100.00 | WP_002560998.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LEMJHGCK_44 | 61880 | 60300 | GroEL | 0.0 | folding of newly synthesized proteins, preventing misfolding and aggregation | 86.72 | 96.70 | BAA04222 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| LEMJHGCK_52 | 696 | 1276 | repUS2 | 584 / 681 | 80.48 | BFU30316 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LEMJHGCK_1 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_2 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_3 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_4 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_5 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_6 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_7 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_8 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_9 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_10 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_11 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_12 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_13 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_14 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_15 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_16 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_17 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_18 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_19 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_20 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_21 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_22 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_23 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_24 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_25 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_26 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_27 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_28 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_29 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_30 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_31 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_32 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_33 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_34 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_35 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_36 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_37 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_38 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_39 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_40 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_41 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_42 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_43 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_44 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_45 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_47 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_48 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_49 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_50 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_51 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_52 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_53 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_54 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_55 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_56 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_57 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_58 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_59 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_60 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_61 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_62 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_63 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_64 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_65 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_66 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_67 | 1.0 | 0.0 | 1.0 |
| LEMJHGCK_68 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_69 | 0.0 | 1.0 | 0.0 |
| LEMJHGCK_70 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LEMJHGCK_3 | 15089 | 27158 | False | Siphoviridae | VOG0327, |
| LEMJHGCK_7 | 81690 | 89589 | True | Siphoviridae | VOG4551, |
Phigaro v2.4.0 (Database: Jan. 2024 release)