safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
7	0	0	7.00

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
CINNJOPI_22	501417	502382	CfxA2	ARO:3003002	CfxA2	100.00	100.00	AF118110.1
CINNJOPI_11	487224	487961	ErmB	ARO:3000375	ErmB	97.96	98.79	AF242872.1
CINNJOPI_48	166387	167277	CblA-1	ARO:3002999	CblA-1	99.66	100.00	GQ343019.1
CINNJOPI_49	32809	34440	ErmF	ARO:3000498	ErmF	99.62	204.14	M17124.1
CINNJOPI_49	31460	32596	tet(X)	ARO:3000205	tet(X)	99.74	97.42	M37699.1
CINNJOPI_49	6023	7948	tet(Q)	ARO:3000191	tet(Q)	96.41	97.56	Z21523.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
CINNJOPI_49	32809	33609	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.00	100.00	M17808
CINNJOPI_49	31430	32596	tet(X)	Doxycycline, Tetracycline, Minocycline, Tigecycline	99.91	100.00	M37699
CINNJOPI_22	501417	502382	cfxA3	Ampicillin	99.90	100.00	AF472622
CINNJOPI_22	501417	502382	cfxA4	Unknown Beta-lactam	99.90	100.00	AY769933
CINNJOPI_22	501417	502382	cfxA5	Unknown Beta-lactam	99.90	100.00	AY769934
CINNJOPI_22	501417	502382	cfxA	Cefoxitin, Ampicillin	99.90	100.00	U38243
CINNJOPI_49	6023	7948	tet(Q)	Doxycycline, Tetracycline, Minocycline	99.84	100.00	L33696
CINNJOPI_11	487224	487961	erm(B)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.73	100.00	U18931

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
CINNJOPI_22	501420	502382	cfxA	CfxA family broad-spectrum class A beta-lactamase	EXACTX	100.00	100.00	WP_004339683.1
CINNJOPI_49	32812	33609	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	EXACTX	100.00	100.00	WP_002682030.1
CINNJOPI_49	31430	32593	tet(X2)	tetracycline-inactivating monooxygenase Tet(X2)	BLASTX	99.74	100.00	WP_008651082.1
CINNJOPI_48	166390	167277	cblA	CblA family class A beta-lactamase	BLASTX	99.66	100.00	WP_005827792.1
CINNJOPI_49	6026	7948	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	99.53	100.00	WP_063856407.1
CINNJOPI_11	487224	487958	erm(B)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	BLASTX	99.18	100.00	WP_002292226.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
No hit found

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
CINNJOPI_35	1	564	repUS2	566 / 681	98.76	BFU30316

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
CINNJOPI_1	0.0	1.0	0.0
CINNJOPI_2	0.0	1.0	0.0
CINNJOPI_3	0.0	1.0	0.0
CINNJOPI_4	0.0	1.0	0.0
CINNJOPI_5	0.0	1.0	0.0
CINNJOPI_6	0.0	1.0	0.0
CINNJOPI_7	0.0	1.0	0.0
CINNJOPI_8	0.0	1.0	0.0
CINNJOPI_9	1.0	0.0	1.0
CINNJOPI_10	0.0	1.0	0.0
CINNJOPI_11	0.0	1.0	0.0
CINNJOPI_12	1.0	0.0	1.0
CINNJOPI_13	1.0	0.0	1.0
CINNJOPI_14	1.0	0.0	1.0
CINNJOPI_15	1.0	0.0	1.0
CINNJOPI_16	1.0	0.0	1.0
CINNJOPI_17	0.0	1.0	0.0
CINNJOPI_18	1.0	0.0	1.0
CINNJOPI_19	0.0	1.0	0.0
CINNJOPI_20	1.0	0.0	1.0
CINNJOPI_21	0.0	1.0	0.0
CINNJOPI_22	0.0	1.0	0.0
CINNJOPI_23	1.0	0.0	1.0
CINNJOPI_25	1.0	0.0	1.0
CINNJOPI_26	1.0	0.0	1.0
CINNJOPI_27	1.0	0.0	1.0
CINNJOPI_28	1.0	0.0	1.0
CINNJOPI_29	1.0	0.0	1.0
CINNJOPI_30	1.0	0.0	1.0
CINNJOPI_31	1.0	0.0	1.0
CINNJOPI_32	0.0	1.0	0.0
CINNJOPI_33	0.0	1.0	0.0
CINNJOPI_34	1.0	0.0	1.0
CINNJOPI_35	1.0	0.0	1.0
CINNJOPI_36	1.0	0.0	1.0
CINNJOPI_37	1.0	0.0	1.0
CINNJOPI_38	0.0	1.0	0.0
CINNJOPI_39	1.0	0.0	1.0
CINNJOPI_40	1.0	0.0	1.0
CINNJOPI_41	1.0	0.0	1.0
CINNJOPI_42	1.0	0.0	1.0
CINNJOPI_43	0.0	1.0	0.0
CINNJOPI_44	0.0	1.0	0.0
CINNJOPI_45	0.0	1.0	0.0
CINNJOPI_46	0.0	1.0	0.0
CINNJOPI_47	0.0	1.0	0.0
CINNJOPI_48	0.0	1.0	0.0
CINNJOPI_49	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
CINNJOPI_49	262672	268420	True	Unknown	VOG8607,

Phigaro v2.4.0 (Database: Jan. 2024 release)