Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 23 | 29 | 37.01 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| NOEEGLPI_1 | 1502025 | 1502864 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_011226733 |
| NOEEGLPI_1 | 245795 | 247054 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011681445 |
| NOEEGLPI_1 | 1098546 | 1099139 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| NOEEGLPI_1 | 1098546 | 1099139 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| NOEEGLPI_1 | 1098546 | 1099139 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| NOEEGLPI_1 | 1097677 | 1098546 | rfbA | 0.0 | Capsule | Immune modulation | 99.54 | 100.00 | WP_011681286 |
| NOEEGLPI_1 | 751988 | 753646 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| NOEEGLPI_1 | 244822 | 245798 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.39 | 99.90 | WP_011681444 |
| NOEEGLPI_1 | 243836 | 244804 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | WP_011681443 |
| NOEEGLPI_1 | 1237314 | 1237763 | STU_RS14620 | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | WP_011226042 |
| NOEEGLPI_1 | 1233904 | 1234644 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 99.19 | 100.00 | WP_011681174 |
| NOEEGLPI_1 | 1063019 | 1063780 | srtA | 0.0 | Sortase A | Exoenzyme | 99.08 | 100.00 | - |
| NOEEGLPI_1 | 569204 | 569994 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.99 | 100.00 | - |
| NOEEGLPI_1 | 1233904 | 1234644 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | WP_011226046 |
| NOEEGLPI_1 | 1236309 | 1237305 | STU_RS14625* | 0.0 | Capsule | Immune modulation | 98.39 | 100.00 | - |
| NOEEGLPI_1 | 1233904 | 1234639 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 98.37 | 98.13 | WP_011227246 |
| NOEEGLPI_1 | 243119 | 243834 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 98.32 | 99.86 | WP_011681442 |
| NOEEGLPI_1 | 1231001 | 1232461 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011681177 |
| NOEEGLPI_1 | 242003 | 242773 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.80 | 99.23 | WP_011681440 |
| NOEEGLPI_1 | 247173 | 248024 | rfbD | 0.0 | Capsule | Immune modulation | 97.30 | 100.00 | WP_014621820 |
| NOEEGLPI_1 | 1232462 | 1233193 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 97.13 | 100.00 | WP_011226048 |
| NOEEGLPI_1 | 1231001 | 1232461 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 97.12 | 100.21 | WP_011226049 |
| NOEEGLPI_1 | 1232462 | 1233193 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 96.45 | 100.00 | WP_011227248 |
| NOEEGLPI_1 | 1231001 | 1232461 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.17 | 100.21 | WP_041827043 |
| NOEEGLPI_1 | 1232462 | 1233193 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 96.17 | 100.00 | WP_011681176 |
| NOEEGLPI_1 | 1233202 | 1233894 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 94.95 | 100.00 | WP_011226047 |
| NOEEGLPI_1 | 1233202 | 1233894 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 94.66 | 100.00 | WP_011227247 |
| NOEEGLPI_1 | 1234700 | 1236065 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.51 | 99.85 | WP_011681173 |
| NOEEGLPI_1 | 1234700 | 1236067 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 94.01 | 100.00 | WP_011226045 |
| NOEEGLPI_1 | 1233202 | 1233894 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 92.64 | 100.00 | WP_011681175 |
| NOEEGLPI_1 | 107105 | 108408 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.02 | 99.46 | WP_000022832 |
| NOEEGLPI_1 | 1639291 | 1640301 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| NOEEGLPI_1 | 1034206 | 1035489 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.65 | 99.77 | WP_000107753 |
| NOEEGLPI_1 | 1099569 | 1100615 | rfbB | 0.0 | Capsule | Immune modulation | 80.52 | 100.00 | WP_009659423 |
| NOEEGLPI_1 | 1237763 | 1238070 | STU_RS14615 | 2.34e-133 | Capsule | Immune modulation | 95.13 | 63.37 | WP_011226041 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| NOEEGLPI_1 | 1060332 | 1059349 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| NOEEGLPI_1 | 205349 | 206422 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| NOEEGLPI_1 | 403085 | 405388 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.23 | 99.22 | AAK74619 |
| NOEEGLPI_1 | 24802 | 25770 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| NOEEGLPI_1 | 689635 | 688154 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 92.31 | 100.00 | ABJ54480 |
| NOEEGLPI_1 | 1379914 | 1382160 | metE | 0.0 | methionine biosynthesis enzymes | 91.86 | 100.00 | AAK74738 |
| NOEEGLPI_1 | 1654604 | 1655560 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 90.28 | 98.46 | ABF36968 |
| NOEEGLPI_1 | 289066 | 287915 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| NOEEGLPI_1 | 261061 | 259073 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| NOEEGLPI_1 | 1076527 | 1078257 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 87.69 | 100.00 | AAK34199 |
| NOEEGLPI_1 | 656719 | 655241 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.42 | 100.00 | ADE32434 |
| NOEEGLPI_1 | 107105 | 108406 | Eno | 0.0 | enolase | 86.21 | 99.77 | ABP90468 |
| NOEEGLPI_1 | 642027 | 640135 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 85.89 | 98.90 | ABP91129 |
| NOEEGLPI_1 | 1521657 | 1522763 | recA | 0.0 | DNA recombination and repair | 84.91 | 95.36 | AAL00560 |
| NOEEGLPI_1 | 1385085 | 1383376 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| NOEEGLPI_1 | 883233 | 882223 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| NOEEGLPI_1 | 952991 | 954352 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| NOEEGLPI_1 | 832939 | 834831 | PepO | 0.0 | endopeptidase | 80.03 | 100.00 | ABF32980 |
| NOEEGLPI_1 | 92666 | 93556 | bglB | 1.30e-178 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| NOEEGLPI_1 | 816540 | 817475 | SP_0676 | 7.21e-168 | transcription facot | 81.73 | 96.89 | AAK74821 |
| NOEEGLPI_1 | 1382352 | 1383209 | metF | 8.43e-165 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| NOEEGLPI_1 | 501598 | 500894 | covR | 1.04e-130 | responder protein | 85.11 | 100.00 | AAK71319 |
| NOEEGLPI_1 | 717736 | 718407 | rr01 | 4.04e-126 | response regulator | 84.82 | 99.56 | CAB54566 |
| NOEEGLPI_1 | 501598 | 500894 | walR | 5.21e-121 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| NOEEGLPI_1 | 1389038 | 1389706 | CiaR | 8.18e-121 | two-component response regulator | 86.10 | 99.55 | AAK74935 |
| NOEEGLPI_1 | 1389038 | 1389709 | ciaR | 9.40e-119 | two component system response regulator | 83.93 | 99.12 | AIK75708 |
| NOEEGLPI_1 | 1411996 | 1411310 | CovR | 9.82e-118 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| NOEEGLPI_1 | 1522946 | 1523320 | SpxA2 | 1.13e-69 | transcriptional regulator | 91.20 | 94.70 | ERL21461 |
| NOEEGLPI_1 | 941607 | 941206 | SpxA1 | 3.67e-66 | transcriptional regulator | 80.60 | 100.00 | ERL19273 |
| NOEEGLPI_1 | 1034048 | 1033548 | RopE (M5005_Spy_1611) | 4.80e-75 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| NOEEGLPI_1 | 403085 | 405382 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| NOEEGLPI_1 | 847368 | 848387 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| NOEEGLPI_1 | 1723821 | 1724673 | rep37 | 855 / 948 | 94.85 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NOEEGLPI_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NOEEGLPI_1 | 516572 | 557963 | False | Siphoviridae | VOG4134, VOG5284, VOG3300, VOG3299, VOG4649, VOG0054, VOG0641, VOG3298, VOG4782, VOG0647, VOG4599, VOG4605, VOG6163, VOG0209, VOG4586, VOG0207, VOG0205, VOG0204, VOG4553, VOG4573, VOG4556, VOG0202, VOG1886, VOG4581, VOG4841, VOG0198, VOG0658, VOG0657, VOG6495, VOG0152, VOG6140, VOG6414, VOG0656, VOG3468, VOG0655, VOG4609, VOG4632, VOG6545, VOG0322, VOG6419, VOG6420, VOG0321, VOG0651, VOG0650, VOG4552, VOG3622, VOG6127, VOG4602, VOG4040, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)