Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LABOCKOM_1 | 3773755 | 3775188 | lmrB | ARO:3002813 | lmrB | 99.16 | 100.00 | JYFL01000006.1 |
| LABOCKOM_1 | 3725420 | 3726013 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| LABOCKOM_1 | 3446545 | 3448191 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.18 | NC_000964.3 |
| LABOCKOM_1 | 3141477 | 3144047 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| LABOCKOM_1 | 2663879 | 2664217 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| LABOCKOM_1 | 2663562 | 2663879 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| LABOCKOM_1 | 1761394 | 1762563 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| LABOCKOM_1 | 1588609 | 1589811 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| LABOCKOM_1 | 1568812 | 1569666 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| LABOCKOM_1 | 26288 | 27664 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.35 | 100.00 | X08034 |
| LABOCKOM_1 | 1568812 | 1569664 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| LABOCKOM_1 | 3789335 | 3790254 | mph(K) | Spiramycin, Telithromycin | 97.61 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| LABOCKOM_1 | 26288 | 27661 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| LABOCKOM_1 | 3446545 | 3448188 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| LABOCKOM_1 | 1568812 | 1569663 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| LABOCKOM_1 | 29645 | 30163 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| LABOCKOM_1 | 963721 | 964659 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| LABOCKOM_1 | 521298 | 522440 | capA | 0.0 | Capsule | Immune modulation | 99.65 | 100.00 | NP_391469 |
| LABOCKOM_1 | 519634 | 520815 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| LABOCKOM_1 | 520830 | 521279 | capC | 0.0 | Capsule | Immune modulation | 99.56 | 100.00 | NP_391470 |
| LABOCKOM_1 | 1967038 | 1967679 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| LABOCKOM_1 | 960049 | 960834 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| LABOCKOM_1 | 2100804 | 2102567 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| LABOCKOM_1 | 964679 | 971815 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.80 | 100.00 | NP_391076 |
| LABOCKOM_1 | 960860 | 962056 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| LABOCKOM_1 | 962074 | 963693 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.84 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| LABOCKOM_1 | 3977450 | 3976314 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| LABOCKOM_1 | 4092657 | 4091788 | pdxS | 5.35e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.45 | 98.31 | ACE61268 |
| LABOCKOM_1 | 2343072 | 2342296 | CodY | 3.33e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| LABOCKOM_1 | 672393 | 671824 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| LABOCKOM_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| LABOCKOM_1 | 1370751 | 1395221 | False | Myoviridae | VOG0720, VOG4555, VOG4553, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136, VOG4574, VOG6116 |
| LABOCKOM_1 | 2694018 | 2720111 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| LABOCKOM_1 | 3897013 | 3920191 | False | Myoviridae | VOG4705, VOG3390, VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG5668, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688 |
| LABOCKOM_1 | 3935182 | 3943142 | False | Unknown | VOG1292, VOG1309, VOG0083, VOG1878, VOG3549, VOG8241, VOG9667, VOG9667, VOG8483 |
Phigaro v2.4.0 (Database: Jan. 2024 release)