safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	34	25	42.20

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
CFJCMAAE_1	353260	354099	cbpD	0.0	Autolysin	Exoenzyme	100.00	100.00	WP_011226733
CFJCMAAE_1	1618073	1618924	rfbD	0.0	Capsule	Immune modulation	100.00	100.00	WP_014621820
CFJCMAAE_1	1429076	1429837	srtA	0.0	Sortase A	Exoenzyme	100.00	100.00	-
CFJCMAAE_1	1271533	1272456	STR_RS05205	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227237
CFJCMAAE_1	1273387	1274469	STR_RS05215	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227239
CFJCMAAE_1	1274559	1275533	STR_RS05220	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227240
CFJCMAAE_1	1279015	1279614	STR_RS05245	0.0	Capsule	Immune modulation	100.00	100.00	WP_196767671
CFJCMAAE_1	1393738	1394331	STR_RS05880	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
CFJCMAAE_1	1605598	1607343	STR_RS06885	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227426
CFJCMAAE_1	1607345	1609045	STR_RS06890	0.0	Capsule	Immune modulation	100.00	100.00	WP_041827077
CFJCMAAE_1	1610265	1611071	STR_RS06900	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227429
CFJCMAAE_1	1611071	1612012	STR_RS06905	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227430
CFJCMAAE_1	1612009	1613157	STR_RS06910	0.0	Capsule	Immune modulation	100.00	100.00	WP_011227431
CFJCMAAE_1	1162418	1164076	fbp54	0.0	FBPs	Adherence	99.94	100.00	WP_011227179
CFJCMAAE_1	1281941	1283395	STR_RS05265	0.0	Capsule	Immune modulation	99.93	100.00	WP_041827043
CFJCMAAE_1	1609063	1610265	STR_RS06895	0.0	Capsule	Immune modulation	99.92	100.00	WP_011227428
CFJCMAAE_1	1279751	1280500	STR_RS05250	0.0	Capsule	Immune modulation	99.87	100.00	WP_011227246
CFJCMAAE_1	1270105	1271525	STR_RS05200	0.0	Capsule	Immune modulation	99.86	99.93	WP_011227236
CFJCMAAE_1	1281210	1281940	STR_RS05260	0.0	Capsule	Immune modulation	99.86	99.86	WP_011227248
CFJCMAAE_1	1393738	1394331	STER_RS06030	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
CFJCMAAE_1	1393738	1394331	STU_RS15260	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
CFJCMAAE_1	1276210	1277326	STR_RS05230	0.0	Capsule	Immune modulation	99.82	99.82	WP_148148887
CFJCMAAE_1	1267354	1268411	waaB	0.0	Capsule	Immune modulation	99.81	99.91	WP_157822584
CFJCMAAE_1	1277894	1278852	STR_RS05240	0.0	Capsule	Immune modulation	99.79	99.90	WP_041827042
CFJCMAAE_1	1275539	1276017	STR_RS05225	0.0	Capsule	Immune modulation	99.79	99.79	WP_041827041
CFJCMAAE_1	1607345	1609045	STER_RS07050	0.0	Capsule	Immune modulation	99.71	100.00	WP_041827753
CFJCMAAE_1	1280510	1281201	STR_RS05255	0.0	Capsule	Immune modulation	99.71	99.86	WP_011227247
CFJCMAAE_1	1272416	1273362	STR_RS05210	0.0	Capsule	Immune modulation	99.58	99.58	WP_011227238
CFJCMAAE_1	1609063	1610265	STER_RS07055	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681436
CFJCMAAE_1	1610265	1611071	STER_RS07060	0.0	Capsule	Immune modulation	99.50	100.00	WP_011681437
CFJCMAAE_1	1611071	1612012	STER_RS07065	0.0	Capsule	Immune modulation	99.47	100.00	WP_011681438
CFJCMAAE_1	1280510	1281201	STU_RS14640	0.0	Capsule	Immune modulation	99.42	99.86	WP_011226047
CFJCMAAE_1	1347702	1348492	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	99.37	100.00	-
CFJCMAAE_1	1612009	1613157	STER_RS07070	0.0	Capsule	Immune modulation	99.22	100.00	WP_011681439
CFJCMAAE_1	1394331	1395200	rfbA	0.0	Capsule	Immune modulation	99.19	100.00	WP_011681286
CFJCMAAE_1	1281210	1281940	STU_RS14645	0.0	Capsule	Immune modulation	98.77	99.86	WP_011226048
CFJCMAAE_1	1279765	1280500	STER_RS05305	0.0	Capsule	Immune modulation	98.37	99.33	WP_011681174
CFJCMAAE_1	1605598	1607343	STER_RS07045	0.0	Capsule	Immune modulation	98.22	100.00	WP_011681434
CFJCMAAE_1	1281210	1281940	STER_RS05315	0.0	Capsule	Immune modulation	98.22	99.86	WP_011681176
CFJCMAAE_1	1281941	1283395	STU_RS14650	0.0	Capsule	Immune modulation	98.01	100.00	WP_011226049
CFJCMAAE_1	1279765	1280500	STU_RS14635	0.0	Capsule	Immune modulation	97.83	99.33	WP_011226046
CFJCMAAE_1	1616696	1617954	STER_RS07100	0.0	Capsule	Immune modulation	97.70	99.92	WP_011681445
CFJCMAAE_1	1281941	1283395	STER_RS05320	0.0	Capsule	Immune modulation	96.99	99.79	WP_011681177
CFJCMAAE_1	1280510	1281201	STER_RS05310	0.0	Capsule	Immune modulation	89.75	99.86	WP_011681175
CFJCMAAE_1	906806	908109	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.54	WP_000022832
CFJCMAAE_1	109085	110095	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.92	99.51	WP_002986042
CFJCMAAE_1	1610265	1611071	SGO_RS05020	0.0	Capsule	Immune modulation	82.90	100.00	WP_012000440
CFJCMAAE_1	452776	454059	tig/ropA	0.0	Trigger factor	Stress survival	80.65	99.77	WP_000107753
CFJCMAAE_1	1392263	1393309	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
CFJCMAAE_1	1616001	1616699	STER_RS07095	2.92e-152	Capsule	Immune modulation	81.15	70.35	WP_011681444
CFJCMAAE_1	1277602	1277838	STR_RS05235*	6.59e-119	Capsule	Immune modulation	100.00	100.00	-
CFJCMAAE_1	1615181	1615647	STER_RS07085	0.0	Capsule	Immune modulation	94.02	65.13	WP_011681442
CFJCMAAE_1	1609528	1610265	SGO_RS05025	0.0	Capsule	Immune modulation	85.23	60.29	WP_012000441

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
CFJCMAAE_1	1015134	1016207	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
CFJCMAAE_1	824609	825577	spr0084	0.0	pathogenitcity related	93.19	98.48	AAK98888
CFJCMAAE_1	1780182	1782485	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.58	99.22	AAK74619
CFJCMAAE_1	1035652	1037898	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
CFJCMAAE_1	372880	373872	recA	0.0	DNA recombination and repair	91.24	85.31	AAL00560
CFJCMAAE_1	1519740	1520828	SPD_0310	0.0	UPF0371 protein SPD_0310	90.63	73.48	ABJ54480
CFJCMAAE_1	336783	337754	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
CFJCMAAE_1	1666404	1665253	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
CFJCMAAE_1	1631948	1629966	uvrB	0.0	excinuclease ABC, B subunit	88.96	99.70	AE014259_2
CFJCMAAE_1	1416340	1414610	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	87.87	100.00	AAK34199
CFJCMAAE_1	298552	297074	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
CFJCMAAE_1	283824	282139	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.48	88.09	ABP91129
CFJCMAAE_1	906806	908107	Eno	0.0	enolase	86.21	99.77	ABP90468
CFJCMAAE_1	1040823	1039114	PGM	0.0	phosphoglucomutase	83.71	99.65	ACH87041
CFJCMAAE_1	150764	151774	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
CFJCMAAE_1	892388	893278	bglB	2.81e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
CFJCMAAE_1	1432715	1433503	ldh(Sp_1220)	1.41e-174	L-lactate dehydrogenase	95.06	80.18	AAK75326
CFJCMAAE_1	1101437	1100502	SP_0676	4.61e-167	transcription facot	81.73	96.89	AAK74821
CFJCMAAE_1	1038090	1038947	metF	8.35e-165	methionine biosynthesis enzymes	86.36	99.31	AAK74739
CFJCMAAE_1	1320017	1319316	covR	8.99e-129	responder protein	84.19	99.57	AAK71319
CFJCMAAE_1	1485684	1485013	rr01	1.16e-125	response regulator	84.38	99.56	CAB54566
CFJCMAAE_1	1320017	1319316	walR	6.51e-119	DNA-binding response regulator	80.34	99.57	ANC99504
CFJCMAAE_1	1044775	1045425	CiaR	1.34e-116	two-component response regulator	86.17	96.88	AAK74935
CFJCMAAE_1	1044775	1045425	ciaR	6.89e-114	two component system response regulator	83.87	96.02	AIK75708
CFJCMAAE_1	1200840	1201241	SpxA1	3.63e-66	transcriptional regulator	80.60	100.00	ERL19273
CFJCMAAE_1	454217	454717	RopE (M5005_Spy_1611)	1.29e-74	probable DNA-directed RNA polymerase subunit delta	80.84	87.43	AAK34603
CFJCMAAE_1	1780182	1782479	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.55	98.08	MYN69560
CFJCMAAE_1	186617	185598	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.18	100.00	ABJ55451
CFJCMAAE_1	1189478	1188555	mnmE	0.0	central tRNA-modifying GTPase	81.94	67.40	ABP90420

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
CFJCMAAE_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found