safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
MGCBMMPO_1	2735277	2736131	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
MGCBMMPO_1	2716568	2717770	blt	ARO:3003006	blt	100.00	100.00	L32599.1
MGCBMMPO_1	2494249	2495418	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
MGCBMMPO_1	1376452	1376769	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
MGCBMMPO_1	1376114	1376452	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
MGCBMMPO_1	916373	918943	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
MGCBMMPO_1	604333	605976	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
MGCBMMPO_1	338754	339347	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
MGCBMMPO_1	288251	289684	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
MGCBMMPO_1	275436	276356	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
MGCBMMPO_1	2735277	2736131	aadK	Streptomycin	100.00	100.00	M26879
MGCBMMPO_1	275436	276356	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
MGCBMMPO_1	4187302	4188678	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
MGCBMMPO_1	2735280	2736131	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
MGCBMMPO_1	275436	276353	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
MGCBMMPO_1	4184784	4185302	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
MGCBMMPO_1	4187305	4188678	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
MGCBMMPO_1	604336	605976	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
MGCBMMPO_1	2050934	2053528	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
MGCBMMPO_1	3697441	3698583	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
MGCBMMPO_1	3699066	3700247	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
MGCBMMPO_1	3698602	3699051	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
MGCBMMPO_1	2004276	2006039	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
MGCBMMPO_1	3291106	3291891	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
MGCBMMPO_1	3287270	3288208	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
MGCBMMPO_1	3289884	3291080	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
MGCBMMPO_1	3288236	3289855	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
MGCBMMPO_1	3280114	3287250	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
MGCBMMPO_1	2294902	2295543	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MGCBMMPO_1	132472	133608	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
MGCBMMPO_1	18664	19533	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
MGCBMMPO_1	1689716	1690492	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
MGCBMMPO_1	3545829	3546398	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MGCBMMPO_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
MGCBMMPO_1	1320167	1346275	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
MGCBMMPO_1	2666639	2696883	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600

Phigaro v2.4.0 (Database: Jan. 2024 release)