Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EFFGILBO_1 | 2610653 | 2611507 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| EFFGILBO_1 | 2593496 | 2594698 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| EFFGILBO_1 | 2352208 | 2353377 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| EFFGILBO_1 | 1405088 | 1405405 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| EFFGILBO_1 | 1404750 | 1405088 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EFFGILBO_1 | 895468 | 898038 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| EFFGILBO_1 | 588529 | 590175 | vmlR | ARO:3004476 | vmlR | 99.45 | 100.18 | NC_000964.3 |
| EFFGILBO_1 | 343295 | 343888 | tmrB | ARO:3003059 | tmrB | 98.98 | 100.00 | AL009126.3 |
| EFFGILBO_1 | 292736 | 294169 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| EFFGILBO_1 | 279921 | 280841 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EFFGILBO_1 | 2610653 | 2611507 | aadK | Streptomycin | 98.95 | 100.00 | M26879 |
| EFFGILBO_1 | 279921 | 280841 | mph(K) | Spiramycin, Telithromycin | 97.83 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EFFGILBO_1 | 588532 | 590175 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.45 | 100.00 | WP_003234144.1 |
| EFFGILBO_1 | 2610656 | 2611507 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| EFFGILBO_1 | 279921 | 280838 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| EFFGILBO_1 | 4084433 | 4084951 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| EFFGILBO_1 | 2062021 | 2064618 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.06 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EFFGILBO_1 | 3593749 | 3594930 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| EFFGILBO_1 | 2154889 | 2155530 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| EFFGILBO_1 | 3169242 | 3170438 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| EFFGILBO_1 | 3170464 | 3171249 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.24 | 100.00 | NP_391080 |
| EFFGILBO_1 | 3166628 | 3167566 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| EFFGILBO_1 | 2016260 | 2018023 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| EFFGILBO_1 | 3592124 | 3593266 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| EFFGILBO_1 | 3593285 | 3593734 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| EFFGILBO_1 | 3159472 | 3166608 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.44 | 100.00 | NP_391076 |
| EFFGILBO_1 | 3167594 | 3169213 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.35 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EFFGILBO_1 | 132416 | 133552 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| EFFGILBO_1 | 18601 | 19470 | pdxS | 3.06e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EFFGILBO_1 | 1716531 | 1717307 | CodY | 3.34e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EFFGILBO_1 | 3432417 | 3432986 | ClpP | 2.47e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EFFGILBO_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EFFGILBO_1 | 1244166 | 1253308 | False | Siphoviridae | VOG4602, VOG3304, VOG9576, VOG9667, VOG9667, VOG8241, VOG4552, VOG4600, VOG1309, VOG4566, VOG0189 |
| EFFGILBO_1 | 1256109 | 1283484 | False | Myoviridae | VOG5447, VOG4972, VOG0796, VOG10227, VOG0720, VOG4555, VOG4572, VOG1643, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136 |
| EFFGILBO_1 | 1347331 | 1375513 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705, VOG0753 |
| EFFGILBO_1 | 2528698 | 2561161 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG2381, VOG4688, VOG4773, VOG10227, VOG0796, VOG4585, VOG0198, VOG4058, VOG4693, VOG0189, VOG11060 |
Phigaro v2.4.0 (Database: Jan. 2024 release)