Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 7 | 10 | 4 | 12.85 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FCNDMMGO_1 | 3536074 | 3536412 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| FCNDMMGO_1 | 3535757 | 3536074 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| FCNDMMGO_1 | 2448687 | 2449889 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| FCNDMMGO_1 | 627484 | 628404 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| FCNDMMGO_1 | 32256 | 34826 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FCNDMMGO_1 | 627484 | 628404 | mph(K) | Spiramycin, Telithromycin | 98.48 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FCNDMMGO_1 | 627487 | 628404 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| FCNDMMGO_1 | 339693 | 341048 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 92.56 | 83.55 | WP_003234144.1 |
| FCNDMMGO_1 | 2965452 | 2968046 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.30 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FCNDMMGO_1 | 2861797 | 2862438 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| FCNDMMGO_1 | 1876809 | 1877594 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| FCNDMMGO_1 | 1440394 | 1441575 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| FCNDMMGO_1 | 1880481 | 1881419 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.57 | 100.00 | NP_391077 |
| FCNDMMGO_1 | 1442058 | 1443200 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| FCNDMMGO_1 | 1441590 | 1442039 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| FCNDMMGO_1 | 3018260 | 3020023 | dep/capD | 0.0 | Capsule | Immune modulation | 99.09 | 100.00 | NP_389723 |
| FCNDMMGO_1 | 1877620 | 1878816 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| FCNDMMGO_1 | 1881439 | 1888575 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.86 | 100.00 | NP_391076 |
| FCNDMMGO_1 | 1878834 | 1880453 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.70 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FCNDMMGO_1 | 805345 | 804209 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| FCNDMMGO_1 | 919381 | 918512 | pdxS | 2.93e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| FCNDMMGO_1 | 3221532 | 3220756 | CodY | 3.20e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| FCNDMMGO_1 | 1583441 | 1582872 | ClpP | 2.37e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FCNDMMGO_3 | 13547 | 13734 | Col(SD853) | 188 / 194 | 85.11 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FCNDMMGO_1 | 0.0 | 1.0 | 0.0 |
| FCNDMMGO_2 | 1.0 | 0.0 | 1.0 |
| FCNDMMGO_3 | 1.0 | 0.0 | 1.0 |
| FCNDMMGO_4 | 1.0 | 0.0 | 1.0 |
| FCNDMMGO_5 | 1.0 | 0.0 | 1.0 |
| FCNDMMGO_6 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FCNDMMGO_1 | 1779343 | 1813642 | False | Siphoviridae | VOG0275, VOG4602, VOG9667, VOG0181, VOG4556, VOG0787, VOG1638, VOG4552, VOG11060, VOG9600, VOG0025, VOG1884, VOG0198, VOG4841, VOG4581, VOG4544, VOG3644, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG4545, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641, VOG4574 |
| FCNDMMGO_1 | 2370357 | 2393323 | False | Siphoviridae | VOG0531, VOG4841, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4660, VOG4612, VOG3390, VOG7896, VOG0641 |
| FCNDMMGO_1 | 3567903 | 3591425 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG3890, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)