Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 7 | 10 | 4 | 12.85 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMBNJEIG_1 | 3616108 | 3616425 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| BMBNJEIG_1 | 3615770 | 3616108 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BMBNJEIG_1 | 3097195 | 3099765 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| BMBNJEIG_1 | 2784235 | 2785860 | vmlR | ARO:3004476 | vmlR | 96.67 | 98.90 | NC_000964.3 |
| BMBNJEIG_1 | 2535477 | 2536070 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| BMBNJEIG_1 | 2473959 | 2474879 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| BMBNJEIG_1 | 357935 | 359104 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BMBNJEIG_1 | 2473959 | 2474879 | mph(K) | Spiramycin, Telithromycin | 97.94 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BMBNJEIG_1 | 2473959 | 2474876 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| BMBNJEIG_1 | 2784238 | 2785860 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.67 | 98.90 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BMBNJEIG_1 | 146169 | 146810 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| BMBNJEIG_1 | 1124927 | 1125865 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BMBNJEIG_1 | 1128752 | 1129537 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| BMBNJEIG_1 | 1636369 | 1637550 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| BMBNJEIG_1 | 1127530 | 1128726 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| BMBNJEIG_1 | 4173241 | 4175004 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| BMBNJEIG_1 | 1634744 | 1635886 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| BMBNJEIG_1 | 1125893 | 1127512 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.70 | 100.00 | NP_391078 |
| BMBNJEIG_1 | 1635905 | 1636354 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| BMBNJEIG_1 | 1117771 | 1124907 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.60 | 100.00 | NP_391076 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BMBNJEIG_1 | 2304837 | 2305973 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BMBNJEIG_1 | 2190886 | 2191755 | pdxS | 3.12e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BMBNJEIG_1 | 3962544 | 3963320 | CodY | 3.40e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BMBNJEIG_1 | 1483327 | 1483896 | ClpP | 2.52e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BMBNJEIG_1 | 0.0 | 1.0 | 0.0 |
| BMBNJEIG_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BMBNJEIG_1 | 704321 | 731354 | False | Myoviridae | VOG5801, VOG4574, VOG0641, VOG5008, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG1643, VOG4572, VOG4555, VOG0720, VOG10227, VOG0796, VOG4681 |
| BMBNJEIG_1 | 733243 | 741172 | False | Unknown | VOG4376, VOG0044, VOG0397, VOG0397, VOG0397, VOG8567, VOG6759, VOG0536, VOG0044, VOG0189, VOG1309, VOG1309, VOG4600 |
| BMBNJEIG_1 | 741625 | 746403 | False | Siphoviridae | VOG4603, VOG4552, VOG4322, VOG0806, VOG6495, VOG9667 |
| BMBNJEIG_1 | 1310463 | 1333122 | False | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG6965, VOG4586, VOG0704, VOG9583, VOG1266, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841 |
| BMBNJEIG_1 | 1341449 | 1353826 | True | Siphoviridae | VOG8387, VOG0944, VOG6495, VOG9667, VOG0944, VOG0944, VOG5532, VOG8387, VOG1563, VOG1563, VOG0275, VOG0275, VOG3490 |
| BMBNJEIG_1 | 1445301 | 1474050 | False | Siphoviridae | VOG0705, VOG7531, VOG11003, VOG5216, VOG0275, VOG8440, VOG0602, VOG4574, VOG5221, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841 |
| BMBNJEIG_1 | 2339304 | 2353237 | False | Myoviridae | VOG4602, VOG8483, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862, VOG7718 |
| BMBNJEIG_1 | 3465616 | 3492964 | False | Siphoviridae | VOG2124, VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG5221, VOG4574, VOG0136, VOG7508 |
| BMBNJEIG_1 | 3560629 | 3585981 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
Phigaro v2.4.0 (Database: Jan. 2024 release)