Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 6 | 10 | 4 | 12.33 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BHOFHODH_1 | 2462151 | 2463317 | bmr | ARO:3003007 | bmr | 98.71 | 99.74 | M33768.1 |
| BHOFHODH_1 | 1467508 | 1468170 | ykkC | ARO:3003063 | ykkC | 98.21 | 196.43 | AL009126.1 |
| BHOFHODH_1 | 965159 | 967783 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 102.10 | AL009126.3 |
| BHOFHODH_1 | 357798 | 358391 | tmrB | ARO:3003059 | tmrB | 96.45 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BHOFHODH_1 | 298130 | 299033 | mph(K) | Spiramycin, Telithromycin | 96.74 | 98.15 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BHOFHODH_1 | 4334737 | 4335255 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 91.91 | 100.00 | WP_003242546.1 |
| BHOFHODH_1 | 298449 | 299030 | mphK | macrolide 2'-phosphotransferase MphK | PARTIALX | 97.94 | 63.40 | WP_003246254.1 |
| BHOFHODH_1 | 624633 | 625730 | vmlR | ABC-F type ribosomal protection protein VmlR | PARTIALX | 96.99 | 66.91 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BHOFHODH_1 | 3300800 | 3301738 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| BHOFHODH_1 | 3818280 | 3819461 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| BHOFHODH_1 | 2187224 | 2187865 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.53 | 100.00 | NP_390062 |
| BHOFHODH_1 | 3304628 | 3305413 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| BHOFHODH_1 | 2015793 | 2017556 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| BHOFHODH_1 | 3301766 | 3303387 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.77 | 100.06 | NP_391078 |
| BHOFHODH_1 | 3303405 | 3304602 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| BHOFHODH_1 | 3293644 | 3300780 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.74 | 100.00 | NP_391076 |
| BHOFHODH_1 | 3816655 | 3817797 | capA | 0.0 | Capsule | Immune modulation | 98.69 | 100.00 | NP_391469 |
| BHOFHODH_1 | 3817816 | 3818265 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BHOFHODH_1 | 102946 | 104082 | tufA | 0.0 | elongation factor Tu | 84.70 | 95.69 | AHM69299 |
| BHOFHODH_1 | 4380405 | 4381274 | pdxS | 3.26e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BHOFHODH_1 | 1787757 | 1788533 | CodY | 2.35e-123 | nutrient-responsive regulator | 80.69 | 100.00 | ACJ80679 |
| BHOFHODH_1 | 3653208 | 3653777 | ClpP | 2.64e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| BHOFHODH_2 | 6543 | 6730 | Col(SD853) | 188 / 194 | 82.98 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BHOFHODH_1 | 0.0 | 1.0 | 0.0 |
| BHOFHODH_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BHOFHODH_1 | 141226 | 145177 | False | Unknown | VOG0083, VOG1309 |
| BHOFHODH_1 | 157807 | 181445 | False | Myoviridae | VOG4773, VOG4688, VOG6965, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG5668, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862, VOG7718 |
| BHOFHODH_1 | 1331636 | 1334679 | False | Siphoviridae | VOG3556, VOG1563, VOG4705, VOG0753, VOG0136 |
| BHOFHODH_1 | 1407477 | 1436816 | False | Myoviridae | VOG8387, VOG0733, VOG0101, VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG7718, VOG4705, VOG0753 |
| BHOFHODH_1 | 2228825 | 2235538 | False | Siphoviridae | VOG4553, VOG4555, VOG0720, VOG5023, VOG0221, VOG3699 |
| BHOFHODH_1 | 2487259 | 2512759 | False | Siphoviridae | VOG5216, VOG6116, VOG8440, VOG0602, VOG4666, VOG5221, VOG4705, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG6163, VOG4545, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841 |
| BHOFHODH_1 | 2533062 | 2549681 | False | Siphoviridae | VOG0371, VOG1093, VOG6888, VOG5543, VOG0026, VOG0026, VOG0026, VOG6497, VOG10937, VOG5753, VOG7255, VOG0052, VOG1563, VOG11003, VOG2215, VOG4551, VOG4813, VOG0025, VOG0189, VOG6168 |
| BHOFHODH_1 | 2707536 | 2738698 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG9315, VOG0796, VOG1993, VOG4673, VOG0143, VOG4693, VOG0189, VOG11060, VOG1309 |
| BHOFHODH_1 | 3397473 | 3409977 | True | Siphoviridae | VOG4574, VOG0641, VOG4581, VOG8387, VOG0944, VOG4841, VOG6965, VOG0198, VOG1884, VOG0025, VOG0025, VOG0737, VOG0181 |
| BHOFHODH_1 | 3511662 | 3531942 | False | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4619, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG1266, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841 |
| BHOFHODH_1 | 3541853 | 3547488 | True | Siphoviridae | VOG0944, VOG6495, VOG9667, VOG1563, VOG1563, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)