Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ELPIFMBE_1 | 2699941 | 2700795 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
| ELPIFMBE_1 | 2682784 | 2683986 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| ELPIFMBE_1 | 2441458 | 2442627 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| ELPIFMBE_1 | 1477187 | 1477504 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| ELPIFMBE_1 | 1476849 | 1477187 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ELPIFMBE_1 | 899221 | 901791 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| ELPIFMBE_1 | 592289 | 593935 | vmlR | ARO:3004476 | vmlR | 99.45 | 100.18 | NC_000964.3 |
| ELPIFMBE_1 | 348912 | 349505 | tmrB | ARO:3003059 | tmrB | 98.98 | 100.00 | AL009126.3 |
| ELPIFMBE_1 | 298357 | 299790 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| ELPIFMBE_1 | 285542 | 286462 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ELPIFMBE_1 | 2699941 | 2700795 | aadK | Streptomycin | 98.95 | 100.00 | M26879 |
| ELPIFMBE_1 | 285542 | 286462 | mph(K) | Spiramycin, Telithromycin | 97.83 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ELPIFMBE_1 | 592292 | 593935 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.45 | 100.00 | WP_003234144.1 |
| ELPIFMBE_1 | 2699944 | 2700795 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| ELPIFMBE_1 | 285542 | 286459 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| ELPIFMBE_1 | 4249085 | 4249603 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ELPIFMBE_1 | 3255734 | 3256672 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.89 | 100.00 | NP_391077 |
| ELPIFMBE_1 | 3256700 | 3258319 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.81 | 100.00 | NP_391078 |
| ELPIFMBE_1 | 3758394 | 3759575 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| ELPIFMBE_1 | 3259570 | 3260355 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| ELPIFMBE_1 | 3248578 | 3255714 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.38 | 100.00 | NP_391076 |
| ELPIFMBE_1 | 2244140 | 2244781 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| ELPIFMBE_1 | 3258348 | 3259544 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.00 | 100.00 | NP_391079 |
| ELPIFMBE_1 | 2067855 | 2069618 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| ELPIFMBE_1 | 3756769 | 3757911 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| ELPIFMBE_1 | 3757930 | 3758379 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ELPIFMBE_1 | 132417 | 133553 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ELPIFMBE_1 | 18601 | 19470 | pdxS | 3.18e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ELPIFMBE_1 | 1788622 | 1789398 | CodY | 3.47e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ELPIFMBE_1 | 3597073 | 3597642 | ClpP | 2.57e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ELPIFMBE_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ELPIFMBE_1 | 1249954 | 1266317 | False | Myoviridae / Siphoviridae | VOG0152, VOG5437, VOG6168, VOG0189, VOG4907, VOG0044, VOG0025, VOG4551, VOG2215, VOG1563, VOG0052, VOG7255, VOG5753, VOG10937, VOG6497, VOG0026, VOG0422, VOG5543 |
| ELPIFMBE_1 | 1286048 | 1325412 | False | Siphoviridae | VOG6430, VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4626, VOG4705, VOG0753, VOG4574, VOG0602, VOG8440, VOG6116, VOG0705, VOG7531, VOG11003, VOG5216, VOG0275, VOG4602, VOG3304, VOG9576, VOG9667, VOG9667, VOG8241, VOG4552, VOG4600, VOG1309, VOG4566, VOG0189 |
| ELPIFMBE_1 | 1328213 | 1355582 | False | Myoviridae | VOG5447, VOG4972, VOG0796, VOG10227, VOG0720, VOG4555, VOG4572, VOG1643, VOG4564, VOG4623, VOG1353, VOG1881, VOG1883, VOG1352, VOG4699, VOG6163, VOG1956, VOG1433, VOG1348, VOG0573, VOG4550, VOG4691, VOG4845, VOG7718, VOG3390, VOG4705, VOG0753, VOG0136 |
| ELPIFMBE_1 | 1419429 | 1447335 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG4705 |
| ELPIFMBE_1 | 2132121 | 2153901 | False | Siphoviridae | VOG4707, VOG0641, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG4545, VOG5910, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544, VOG4581, VOG1183 |
| ELPIFMBE_1 | 2617947 | 2648920 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG2381, VOG4688, VOG4773, VOG10227, VOG0796, VOG4585, VOG0198, VOG4058, VOG4693 |
| ELPIFMBE_1 | 3276575 | 3299394 | False | Siphoviridae | VOG0602, VOG4574, VOG5221, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG11074, VOG4319, VOG9317, VOG0692, VOG2613, VOG3699, VOG5023, VOG0275, VOG1183 |
| ELPIFMBE_1 | 3365811 | 3399205 | False | Siphoviridae | VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG4545, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3644, VOG4544, VOG4581, VOG4841, VOG0275, VOG0198, VOG1884, VOG0025, VOG9600, VOG4566, VOG4552, VOG1638, VOG1638, VOG0787, VOG4556, VOG0181, VOG5353, VOG9667, VOG4602 |
Phigaro v2.4.0 (Database: Jan. 2024 release)