Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KMAIHENH_1 | 2662580 | 2663413 | aadK | ARO:3002627 | aadK | 97.11 | 97.54 | AL009126.1 |
| KMAIHENH_1 | 2645510 | 2646712 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| KMAIHENH_1 | 2472259 | 2473428 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| KMAIHENH_1 | 1362114 | 1362431 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| KMAIHENH_1 | 1361776 | 1362114 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| KMAIHENH_1 | 892548 | 895118 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| KMAIHENH_1 | 581266 | 582912 | vmlR | ARO:3004476 | vmlR | 99.45 | 100.18 | NC_000964.3 |
| KMAIHENH_1 | 324099 | 324692 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| KMAIHENH_1 | 273543 | 274976 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.00 | JYFL01000006.1 |
| KMAIHENH_1 | 260729 | 261649 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| KMAIHENH_1 | 260729 | 261649 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| KMAIHENH_1 | 4200468 | 4201844 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 99.13 | 100.00 | X08034 |
| KMAIHENH_1 | 2662580 | 2663434 | aadK | Streptomycin | 98.25 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| KMAIHENH_1 | 581269 | 582912 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.45 | 100.00 | WP_003234144.1 |
| KMAIHENH_1 | 4200471 | 4201844 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_003242953.1 |
| KMAIHENH_1 | 260729 | 261646 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| KMAIHENH_1 | 2662583 | 2663434 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.18 | 100.00 | WP_003229862.1 |
| KMAIHENH_1 | 4197982 | 4198500 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.06 | 100.00 | WP_003242546.1 |
| KMAIHENH_1 | 2046858 | 2049455 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.76 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| KMAIHENH_1 | 2274957 | 2275598 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| KMAIHENH_1 | 3707328 | 3708509 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| KMAIHENH_1 | 3288538 | 3289323 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| KMAIHENH_1 | 1992626 | 1994389 | dep/capD | 0.0 | Capsule | Immune modulation | 99.26 | 100.00 | NP_389723 |
| KMAIHENH_1 | 3284702 | 3285640 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| KMAIHENH_1 | 3705703 | 3706845 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| KMAIHENH_1 | 3706864 | 3707313 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| KMAIHENH_1 | 3277546 | 3284682 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391076 |
| KMAIHENH_1 | 3287316 | 3288512 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.50 | 100.00 | NP_391079 |
| KMAIHENH_1 | 3285668 | 3287287 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.16 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| KMAIHENH_1 | 132416 | 133552 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| KMAIHENH_1 | 18600 | 19469 | pdxS | 3.14e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| KMAIHENH_1 | 1673571 | 1674347 | CodY | 3.43e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| KMAIHENH_1 | 3550757 | 3551326 | ClpP | 2.54e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| KMAIHENH_1 | 0.0 | 1.0 | 0.0 |
| KMAIHENH_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| KMAIHENH_1 | 1307238 | 1332537 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG3390, VOG7895, VOG0753 |
| KMAIHENH_1 | 2209156 | 2219083 | False | Myoviridae | VOG7567, VOG4570, VOG5722, VOG5779, VOG5721, VOG10940 |
| KMAIHENH_1 | 2228936 | 2243741 | False | Siphoviridae | VOG9312, VOG9312, VOG4910, VOG3389, VOG5042, VOG9712, VOG4626, VOG3390 |
| KMAIHENH_1 | 2793668 | 2820158 | False | Siphoviridae | VOG4972, VOG4574, VOG0641, VOG4705, VOG3390, VOG4612, VOG4626, VOG4619, VOG0801, VOG4545, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG4841, VOG0198, VOG7372, VOG2752 |
| KMAIHENH_1 | 2871347 | 2894795 | False | Myoviridae | VOG0221, VOG6116, VOG4574, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555 |
Phigaro v2.4.0 (Database: Jan. 2024 release)