Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 10 | 3 | 11.18 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FHEPCDHB_1 | 1378684 | 1379001 | ykkD | ARO:3003064 | ykkD | 97.14 | 100.00 | AL009126.1 |
| FHEPCDHB_1 | 1378346 | 1378684 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| FHEPCDHB_1 | 264456 | 265376 | mphK | ARO:3004541 | mphK | 96.41 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| FHEPCDHB_1 | 264456 | 265376 | mph(K) | Spiramycin, Telithromycin | 97.29 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| FHEPCDHB_1 | 264456 | 265373 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 96.41 | 100.00 | WP_003246254.1 |
| FHEPCDHB_1 | 559619 | 561100 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 95.75 | 90.31 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| FHEPCDHB_1 | 3481755 | 3482936 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | NP_391471 |
| FHEPCDHB_1 | 2073949 | 2074590 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| FHEPCDHB_1 | 3072832 | 3073770 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 99.89 | NP_391077 |
| FHEPCDHB_1 | 3075449 | 3076645 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.91 | 100.00 | NP_391079 |
| FHEPCDHB_1 | 1908980 | 1910745 | dep/capD | 0.0 | Capsule | Immune modulation | 98.75 | 100.00 | NP_389723 |
| FHEPCDHB_1 | 3073798 | 3075420 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.71 | 100.00 | NP_391078 |
| FHEPCDHB_1 | 3076671 | 3077458 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.61 | 99.87 | NP_391080 |
| FHEPCDHB_1 | 3481292 | 3481740 | capC | 0.0 | Capsule | Immune modulation | 98.22 | 99.78 | NP_391470 |
| FHEPCDHB_1 | 3065670 | 3072813 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.56 | 99.97 | NP_391076 |
| FHEPCDHB_1 | 3480148 | 3481273 | capA | 0.0 | Capsule | Immune modulation | 96.77 | 99.83 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| FHEPCDHB_1 | 123661 | 124506 | tufA | 5.52e-167 | elongation factor Tu | 86.53 | 71.32 | AHM69299 |
| FHEPCDHB_1 | 9415 | 10062 | pdxS | 1.11e-128 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 86.11 | 73.22 | ACE61268 |
| FHEPCDHB_1 | 1705275 | 1706009 | CodY | 1.70e-117 | nutrient-responsive regulator | 82.04 | 94.59 | ACJ80679 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| FHEPCDHB_3 | 1406 | 1593 | Col(SD853) | 188 / 194 | 82.98 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| FHEPCDHB_1 | 0.0 | 1.0 | 0.0 |
| FHEPCDHB_2 | 1.0 | 0.0 | 1.0 |
| FHEPCDHB_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| FHEPCDHB_1 | 609530 | 617691 | False | Siphoviridae | VOG4602, VOG4662, VOG9667, VOG9667, VOG0650, VOG4552, VOG4600, VOG1309, VOG4566 |
| FHEPCDHB_1 | 624698 | 642213 | False | Siphoviridae | VOG6005, VOG0796, VOG10227, VOG0691, VOG0691, VOG0692, VOG9317, VOG9317, VOG4572, VOG2621, VOG0695, VOG0696, VOG0697, VOG0698, VOG10044, VOG0701, VOG6964, VOG9745, VOG4605, VOG4605, VOG4599 |
| FHEPCDHB_1 | 1322366 | 1348496 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG9315, VOG4773, VOG4773, VOG4685, VOG0749, VOG0749, VOG3890, VOG3890, VOG3891, VOG3892, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG7718, VOG4705 |
| FHEPCDHB_1 | 2317451 | 2343466 | False | Siphoviridae | VOG11003, VOG7531, VOG0705, VOG0705, VOG6116, VOG7508, VOG0602, VOG4574, VOG5221, VOG4705, VOG6243, VOG4612, VOG4612, VOG4626, VOG4619, VOG0801, VOG0801, VOG4545, VOG4545, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5601, VOG4553, VOG4573, VOG4556, VOG1886, VOG1886, VOG4581, VOG4841 |
| FHEPCDHB_1 | 2363640 | 2371380 | False | Siphoviridae | VOG5543, VOG5543, VOG0982, VOG0026, VOG0026, VOG7980, VOG5385, VOG10937, VOG5753 |
Phigaro v2.4.0 (Database: Jan. 2024 release)