Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 10 | 4 | 10.95 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ICHJJLEH_1 | 3791089 | 3792013 | mph(K) | Spiramycin, Telithromycin | 97.19 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ICHJJLEH_1 | 135570 | 135941 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 90.32 | 71.68 | WP_003242546.1 |
| ICHJJLEH_1 | 3504978 | 3505994 | vmlR | ABC-F type ribosomal protection protein VmlR | PARTIALX | 97.05 | 61.79 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ICHJJLEH_1 | 2081422 | 2082065 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.16 | NP_390062 |
| ICHJJLEH_1 | 607583 | 608032 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| ICHJJLEH_1 | 1056289 | 1057229 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.11 | NP_391077 |
| ICHJJLEH_1 | 606385 | 607568 | capB | 0.0 | Capsule | Immune modulation | 98.82 | 100.00 | NP_391471 |
| ICHJJLEH_1 | 608051 | 609195 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| ICHJJLEH_1 | 2232522 | 2234284 | dep/capD | 0.0 | Capsule | Immune modulation | 98.30 | 99.89 | NP_389723 |
| ICHJJLEH_1 | 1057249 | 1064391 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.22 | 99.97 | NP_391076 |
| ICHJJLEH_1 | 1053410 | 1054615 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.09 | 100.17 | NP_391079 |
| ICHJJLEH_1 | 1054633 | 1056261 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.04 | 100.19 | NP_391078 |
| ICHJJLEH_1 | 1052594 | 1053384 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.98 | 100.13 | NP_391080 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ICHJJLEH_1 | 82454 | 81585 | pdxS | 2.96e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ICHJJLEH_1 | 2434310 | 2433534 | CodY | 3.24e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ICHJJLEH_1 | 753388 | 752906 | ClpP | 6.93e-84 | part of proteolytic complex | 83.85 | 82.56 | BAB94595 |
| ICHJJLEH_1 | 2893580 | 2893188 | spxA1 | 2.54e-69 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| ICHJJLEH_1 | 2893580 | 2893188 | spx | 1.52e-67 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| ICHJJLEH_4 | 4428 | 4614 | Col(SD853) | 187 / 194 | 85.56 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ICHJJLEH_1 | 0.0 | 1.0 | 0.0 |
| ICHJJLEH_2 | 1.0 | 0.0 | 1.0 |
| ICHJJLEH_3 | 1.0 | 0.0 | 1.0 |
| ICHJJLEH_4 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ICHJJLEH_1 | 958545 | 988951 | True | Siphoviridae | VOG0181, VOG4556, VOG0787, VOG1638, VOG4552, VOG4566, VOG9600, VOG0025, VOG0025, VOG0025, VOG1884, VOG0198, VOG0275, VOG0275, VOG4841, VOG4581, VOG1886, VOG1886, VOG4556, VOG4556, VOG4568, VOG4568, VOG4553, VOG4553, VOG0204, VOG4589, VOG0704, VOG0704, VOG4586, VOG0541, VOG5910, VOG10611, VOG6163, VOG0801, VOG4619, VOG3462, VOG4619, VOG4660, VOG4660, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641 |
| ICHJJLEH_1 | 1172103 | 1187440 | False | Siphoviridae | VOG0720, VOG0720, VOG4564, VOG4564, VOG4555, VOG1329, VOG3480, VOG4623, VOG0816, VOG0724, VOG0799, VOG2621, VOG2621, VOG0725, VOG1323, VOG4183, VOG9745, VOG4605 |
| ICHJJLEH_1 | 1680822 | 1694629 | False | Myoviridae | VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3890, VOG3891, VOG3892, VOG6163, VOG4885, VOG4885, VOG3894 |
| ICHJJLEH_1 | 2786979 | 2797223 | False | Myoviridae | VOG3891, VOG3890, VOG3890, VOG3890, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG4773, VOG10227, VOG0796, VOG0796, VOG5447, VOG4861 |
Phigaro v2.4.0 (Database: Jan. 2024 release)