Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 10 | 3 | 11.18 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HDILNOAP_1 | 3836198 | 3837001 | mphK | ARO:3004541 | mphK | 96.08 | 87.25 | CP010314.1 |
| HDILNOAP_1 | 3540302 | 3541927 | vmlR | ARO:3004476 | vmlR | 95.93 | 98.90 | NC_000964.3 |
| HDILNOAP_1 | 2722629 | 2722967 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| HDILNOAP_1 | 2722312 | 2722629 | ykkD | ARO:3003064 | ykkD | 97.14 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HDILNOAP_1 | 3836198 | 3837118 | mph(K) | Spiramycin, Telithromycin | 97.40 | 99.89 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HDILNOAP_1 | 3540302 | 3541924 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 95.93 | 98.90 | WP_003234144.1 |
| HDILNOAP_1 | 3836201 | 3837118 | mphK | macrolide 2'-phosphotransferase MphK | INTERNAL_STOP | 86.27 | 100.00 | WP_003246254.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HDILNOAP_1 | 616058 | 617239 | capB | 0.0 | Capsule | Immune modulation | 99.41 | 100.00 | NP_391471 |
| HDILNOAP_1 | 2025267 | 2025909 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| HDILNOAP_1 | 1025775 | 1026713 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| HDILNOAP_1 | 2189154 | 2190918 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| HDILNOAP_1 | 1022901 | 1024097 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| HDILNOAP_1 | 1024126 | 1025747 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391078 |
| HDILNOAP_1 | 1022090 | 1022875 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.60 | 100.00 | NP_391080 |
| HDILNOAP_1 | 617254 | 617703 | capC | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | NP_391470 |
| HDILNOAP_1 | 1026732 | 1033877 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.65 | 99.99 | NP_391076 |
| HDILNOAP_1 | 617722 | 618846 | capA | 0.0 | Capsule | Immune modulation | 96.76 | 99.83 | NP_391469 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HDILNOAP_1 | 82238 | 81369 | pdxS | 4.56e-155 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 82.76 | 98.31 | ACE61268 |
| HDILNOAP_1 | 509834 | 510583 | FbaA | 3.84e-130 | fructose-bisphosphate aldolase | 80.40 | 87.41 | BAB43211 |
| HDILNOAP_1 | 2394722 | 2393946 | CodY | 6.51e-122 | nutrient-responsive regulator | 80.31 | 100.00 | ACJ80679 |
| HDILNOAP_1 | 3978154 | 3977357 | tufA | 1.32e-164 | elongation factor Tu | 82.33 | 67.01 | AHM69299 |
| HDILNOAP_1 | 994351 | 994938 | lipA (SAUSA300_0829) | 3.38e-105 | lipoic acid synthetase | 81.12 | 64.26 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| HDILNOAP_3 | 7867 | 8054 | Col(SD853) | 188 / 194 | 82.98 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HDILNOAP_1 | 0.0 | 1.0 | 0.0 |
| HDILNOAP_2 | 1.0 | 0.0 | 1.0 |
| HDILNOAP_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HDILNOAP_1 | 1727892 | 1735862 | False | Siphoviridae | VOG7255, VOG5753, VOG10937, VOG5385, VOG6497, VOG0026, VOG0026, VOG0982 |
| HDILNOAP_1 | 1756313 | 1782346 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG4545, VOG4545, VOG0801, VOG0801, VOG4619, VOG4619, VOG4626, VOG4612, VOG6243, VOG4705, VOG5221, VOG4574, VOG0602, VOG7508, VOG0705, VOG0705, VOG7531, VOG7531 |
| HDILNOAP_1 | 2753711 | 2777074 | False | Myoviridae | VOG4705, VOG7718, VOG7718, VOG4862, VOG4691, VOG4691, VOG4550, VOG3894, VOG4885, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG3890, VOG3890, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG9315, VOG0796, VOG0796, VOG5447, VOG5447, VOG0189, VOG0189 |
| HDILNOAP_1 | 3458962 | 3476887 | False | Siphoviridae | VOG4707, VOG0641, VOG10944, VOG4599, VOG4605, VOG9745, VOG0701, VOG0701, VOG10044, VOG0698, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG9317, VOG0692, VOG0691, VOG0796 |
| HDILNOAP_1 | 3485451 | 3494518 | False | Siphoviridae | VOG1309, VOG4752, VOG4600, VOG4552, VOG4552, VOG0650, VOG9667, VOG9667, VOG4662, VOG4602, VOG0275, VOG6513 |
Phigaro v2.4.0 (Database: Jan. 2024 release)