Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 10 | 4 | 11.49 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EAELKPII_1 | 3575231 | 3575569 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| EAELKPII_1 | 3574914 | 3575231 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| EAELKPII_1 | 2427398 | 2428600 | blt | ARO:3003006 | blt | 99.00 | 100.00 | L32599.1 |
| EAELKPII_1 | 671583 | 672503 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EAELKPII_1 | 671583 | 672503 | mph(K) | Spiramycin, Telithromycin | 98.48 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EAELKPII_1 | 671586 | 672503 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| EAELKPII_1 | 382198 | 383277 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 93.33 | 65.81 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EAELKPII_1 | 1890665 | 1891450 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| EAELKPII_1 | 1894340 | 1895279 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| EAELKPII_1 | 1483388 | 1484567 | capB | 0.0 | Capsule | Immune modulation | 99.41 | 99.92 | NP_391471 |
| EAELKPII_1 | 2901021 | 2901663 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| EAELKPII_1 | 1485050 | 1486192 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| EAELKPII_1 | 1484582 | 1485031 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| EAELKPII_1 | 3049750 | 3051512 | dep/capD | 0.0 | Capsule | Immune modulation | 98.87 | 99.94 | NP_389723 |
| EAELKPII_1 | 1895299 | 1902442 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.70 | 100.01 | NP_391076 |
| EAELKPII_1 | 1891476 | 1892675 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.67 | 100.08 | NP_391079 |
| EAELKPII_1 | 1892693 | 1894312 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.64 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EAELKPII_1 | 838176 | 837040 | tufA | 0.0 | elongation factor Tu | 84.70 | 95.69 | AHM69299 |
| EAELKPII_1 | 952228 | 951359 | pdxS | 2.97e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EAELKPII_1 | 3259214 | 3258438 | CodY | 3.24e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EAELKPII_1 | 1629921 | 1629352 | ClpP | 2.40e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| EAELKPII_1 | 2280399 | 2281196 | ClpX | 3.92e-154 | part of proteolytic complex | 80.52 | 63.33 | BAF67840 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| EAELKPII_4 | 1730 | 1916 | Col(SD853) | 187 / 194 | 85.56 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EAELKPII_1 | 0.0 | 1.0 | 0.0 |
| EAELKPII_2 | 1.0 | 0.0 | 1.0 |
| EAELKPII_3 | 1.0 | 0.0 | 1.0 |
| EAELKPII_4 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EAELKPII_1 | 303431 | 318683 | False | Siphoviridae | VOG4599, VOG4605, VOG9745, VOG0701, VOG10044, VOG0698, VOG0697, VOG0696, VOG0695, VOG2621, VOG4572, VOG9317, VOG0692, VOG0692, VOG0691, VOG0796 |
| EAELKPII_1 | 327447 | 335770 | False | Siphoviridae | VOG11060, VOG1309, VOG4600, VOG4600, VOG4552, VOG4552, VOG0650, VOG9667, VOG9667, VOG4662, VOG4602, VOG0275 |
| EAELKPII_1 | 2633731 | 2635035 | False | Unknown | VOG4567 |
| EAELKPII_1 | 2644856 | 2669787 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG1886, VOG4556, VOG4573, VOG4553, VOG5601, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG4545, VOG4545, VOG6163, VOG6163, VOG0801, VOG4619, VOG4868, VOG4612, VOG3390, VOG4705, VOG0753, VOG4574, VOG0602, VOG7508, VOG6116, VOG0705 |
| EAELKPII_1 | 3608556 | 3622942 | False | Myoviridae | VOG3892, VOG3891, VOG3890, VOG3890, VOG3890, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)