Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 10 | 4 | 11.87 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CFOBFHCB_1 | 2751159 | 2751497 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| CFOBFHCB_1 | 2750842 | 2751159 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| CFOBFHCB_1 | 3775504 | 3776425 | mph(K) | Spiramycin, Telithromycin | 98.26 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| CFOBFHCB_1 | 198468 | 198839 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.13 | 71.68 | WP_003242546.1 |
| CFOBFHCB_1 | 3506349 | 3507971 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 84.36 | 98.90 | WP_003234144.1 |
| CFOBFHCB_1 | 3775835 | 3776425 | mphK | macrolide 2'-phosphotransferase MphK | PARTIALX | 98.48 | 64.38 | WP_003246254.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| CFOBFHCB_1 | 1124830 | 1125769 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| CFOBFHCB_1 | 1121155 | 1121940 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| CFOBFHCB_1 | 2069940 | 2070582 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| CFOBFHCB_1 | 669343 | 670485 | capA | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_391469 |
| CFOBFHCB_1 | 667682 | 668860 | capB | 0.0 | Capsule | Immune modulation | 99.15 | 99.83 | NP_391471 |
| CFOBFHCB_1 | 668875 | 669324 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| CFOBFHCB_1 | 1121966 | 1123163 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.75 | 100.00 | NP_391079 |
| CFOBFHCB_1 | 1125789 | 1132926 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.71 | 99.96 | NP_391076 |
| CFOBFHCB_1 | 2167308 | 2169071 | dep/capD | 0.0 | Capsule | Immune modulation | 98.64 | 99.94 | NP_389723 |
| CFOBFHCB_1 | 1123181 | 1124802 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| CFOBFHCB_1 | 32813 | 31926 | tufA | 1.47e-166 | elongation factor Tu | 84.46 | 74.62 | AHM69299 |
| CFOBFHCB_1 | 146937 | 146068 | pdxS | 2.94e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| CFOBFHCB_1 | 2438873 | 2438097 | CodY | 3.21e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| CFOBFHCB_1 | 826578 | 826009 | ClpP | 2.38e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| CFOBFHCB_3 | 3317 | 3477 | Col(SD853) | 161 / 194 | 83.85 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| CFOBFHCB_1 | 0.0 | 1.0 | 0.0 |
| CFOBFHCB_2 | 1.0 | 0.0 | 1.0 |
| CFOBFHCB_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| CFOBFHCB_1 | 1024489 | 1056177 | False | Siphoviridae | VOG4602, VOG9667, VOG0181, VOG4556, VOG0787, VOG1638, VOG4552, VOG4566, VOG9600, VOG0025, VOG1884, VOG0198, VOG4841, VOG4581, VOG4544, VOG3644, VOG4556, VOG4568, VOG0083, VOG4553, VOG5361, VOG0204, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG4611, VOG6163, VOG6163, VOG0801, VOG4619, VOG4619, VOG4619, VOG4619, VOG4660, VOG4660, VOG4660, VOG4660, VOG4612, VOG4612, VOG3390, VOG4705, VOG0641 |
| CFOBFHCB_1 | 2784503 | 2800274 | False | Myoviridae | VOG3892, VOG3891, VOG3891, VOG3890, VOG3890, VOG3890, VOG3890, VOG10055, VOG0749, VOG4685, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667, VOG4602 |
| CFOBFHCB_1 | 3907082 | 3932500 | False | Myoviridae | VOG7718, VOG4862, VOG4862, VOG4691, VOG4691, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG3890, VOG10055, VOG4870, VOG1581, VOG0749, VOG4685, VOG4685, VOG4685, VOG6965, VOG4688, VOG4688, VOG4773, VOG10227, VOG9315 |
| CFOBFHCB_1 | 3944276 | 3950850 | False | Siphoviridae | VOG3709, VOG11060, VOG1292, VOG1292, VOG1309, VOG0083, VOG1878 |
Phigaro v2.4.0 (Database: Jan. 2024 release)