Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 10 | 4 | 11.87 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ADJEEICC_1 | 2576692 | 2577612 | mphK | ARO:3004541 | mphK | 97.06 | 100.00 | CP010314.1 |
| ADJEEICC_1 | 1557610 | 1557948 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ADJEEICC_1 | 1557293 | 1557610 | ykkD | ARO:3003064 | ykkD | 97.14 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ADJEEICC_1 | 2576692 | 2577612 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ADJEEICC_1 | 2576695 | 2577612 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.06 | 100.00 | WP_003246254.1 |
| ADJEEICC_1 | 2294553 | 2295908 | vmlR | ABC-F type ribosomal protection protein VmlR | INTERNAL_STOP | 92.34 | 83.55 | WP_003234144.1 |
| ADJEEICC_1 | 2888906 | 2889277 | satA | streptothricin N-acetyltransferase SatA | PARTIALX | 91.13 | 71.68 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ADJEEICC_1 | 3360604 | 3361785 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| ADJEEICC_1 | 838747 | 839388 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.22 | 100.00 | NP_390062 |
| ADJEEICC_1 | 994588 | 996351 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 99.94 | NP_389723 |
| ADJEEICC_1 | 3807011 | 3807949 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.94 | 100.00 | NP_391077 |
| ADJEEICC_1 | 3362270 | 3363412 | capA | 0.0 | Capsule | Immune modulation | 98.86 | 100.00 | NP_391469 |
| ADJEEICC_1 | 3803334 | 3804119 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.60 | 100.00 | NP_391080 |
| ADJEEICC_1 | 3804145 | 3805341 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391079 |
| ADJEEICC_1 | 3361800 | 3362251 | capC | 0.0 | Capsule | Immune modulation | 98.45 | 100.22 | NP_391470 |
| ADJEEICC_1 | 3807969 | 3815110 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.21 | 99.94 | NP_391076 |
| ADJEEICC_1 | 3805359 | 3806983 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.72 | 100.06 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ADJEEICC_1 | 2722938 | 2721889 | tufA | 1.27e-178 | elongation factor Tu | 83.71 | 88.07 | AHM69299 |
| ADJEEICC_1 | 2837132 | 2836482 | pdxS | 1.46e-127 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 86.64 | 73.56 | ACE61268 |
| ADJEEICC_1 | 1239963 | 1239187 | CodY | 3.19e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ADJEEICC_1 | 3505000 | 3504431 | ClpP | 5.93e-97 | part of proteolytic complex | 80.00 | 97.44 | BAB94595 |
| ADJEEICC_1 | 1162127 | 1161171 | cvfA | 6.01e-145 | virulence factor | 80.25 | 61.46 | BAF96735 |
| ADJEEICC_1 | 1162130 | 1161171 | rny (EF3170) | 1.39e-149 | RNase Y | 82.19 | 61.78 | AAO82844 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| ADJEEICC_4 | 1643 | 1810 | Col(SD853) | 168 / 194 | 86.90 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ADJEEICC_1 | 0.0 | 1.0 | 0.0 |
| ADJEEICC_2 | 1.0 | 0.0 | 1.0 |
| ADJEEICC_3 | 1.0 | 0.0 | 1.0 |
| ADJEEICC_4 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ADJEEICC_1 | 1000711 | 1004194 | False | Siphoviridae | VOG9667, VOG9667, VOG0181, VOG4566, VOG4566, VOG0189 |
| ADJEEICC_1 | 1007179 | 1036336 | False | Siphoviridae | VOG4567, VOG8909, VOG0198, VOG0198, VOG0275, VOG0531, VOG6154, VOG4581, VOG1886, VOG4556, VOG4556, VOG4568, VOG4553, VOG4599, VOG0204, VOG1266, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910, VOG7454, VOG0801, VOG4772, VOG4619, VOG4660, VOG4612, VOG4612, VOG3390, VOG7896, VOG0641 |
| ADJEEICC_1 | 1588683 | 1606963 | False | Myoviridae | VOG4705, VOG6163, VOG3892, VOG3891, VOG3890, VOG3890, VOG3890, VOG3890, VOG3890, VOG0749, VOG0749, VOG4685, VOG4773, VOG10227, VOG9315, VOG0796, VOG0796, VOG5447, VOG4843, VOG0189, VOG3490, VOG9667, VOG4602 |
| ADJEEICC_1 | 3702476 | 3732487 | False | Siphoviridae | VOG9667, VOG0181, VOG4556, VOG0787, VOG1638, VOG4552, VOG4566, VOG9600, VOG0025, VOG1884, VOG0198, VOG4841, VOG4581, VOG4544, VOG1886, VOG3644, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612 |
Phigaro v2.4.0 (Database: Jan. 2024 release)