Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 7 | 10 | 4 | 12.85 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DCIBGDOO_1 | 4071969 | 4072889 | mphK | ARO:3004541 | mphK | 97.71 | 100.00 | CP010314.1 |
| DCIBGDOO_1 | 4019585 | 4020178 | tmrB | ARO:3003059 | tmrB | 96.95 | 100.00 | AL009126.3 |
| DCIBGDOO_1 | 3741513 | 3743135 | vmlR | ARO:3004476 | vmlR | 95.75 | 98.72 | NC_000964.3 |
| DCIBGDOO_1 | 3393405 | 3395975 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| DCIBGDOO_1 | 2916093 | 2916431 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DCIBGDOO_1 | 2915776 | 2916093 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DCIBGDOO_1 | 1789602 | 1790771 | bmr | ARO:3003007 | bmr | 98.97 | 100.00 | M33768.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DCIBGDOO_1 | 4071969 | 4072889 | mph(K) | Spiramycin, Telithromycin | 97.94 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DCIBGDOO_1 | 4071972 | 4072889 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.71 | 100.00 | WP_003246254.1 |
| DCIBGDOO_1 | 3741513 | 3743132 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 95.75 | 98.90 | WP_003234144.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DCIBGDOO_1 | 2062306 | 2062947 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.69 | 100.00 | NP_390062 |
| DCIBGDOO_1 | 1012820 | 1013605 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| DCIBGDOO_1 | 513218 | 514398 | capB | 0.0 | Capsule | Immune modulation | 99.49 | 99.92 | NP_391471 |
| DCIBGDOO_1 | 1016492 | 1017430 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| DCIBGDOO_1 | 1013631 | 1014827 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| DCIBGDOO_1 | 514413 | 514862 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| DCIBGDOO_1 | 2280346 | 2282109 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| DCIBGDOO_1 | 514881 | 516023 | capA | 0.0 | Capsule | Immune modulation | 98.78 | 100.00 | NP_391469 |
| DCIBGDOO_1 | 1017450 | 1024586 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.66 | 100.00 | NP_391076 |
| DCIBGDOO_1 | 1014845 | 1016464 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.15 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DCIBGDOO_1 | 4240120 | 4238984 | tufA | 0.0 | elongation factor Tu | 84.70 | 95.69 | AHM69299 |
| DCIBGDOO_1 | 4354173 | 4353304 | pdxS | 3.27e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DCIBGDOO_1 | 2590295 | 2589519 | CodY | 2.35e-123 | nutrient-responsive regulator | 80.69 | 100.00 | ACJ80679 |
| DCIBGDOO_1 | 675934 | 675365 | ClpP | 2.65e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| DCIBGDOO_2 | 4707 | 4894 | Col(SD853) | 188 / 194 | 82.98 | NC015392 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DCIBGDOO_1 | 0.0 | 1.0 | 0.0 |
| DCIBGDOO_2 | 1.0 | 0.0 | 1.0 |
| DCIBGDOO_3 | 0.0 | 1.0 | 0.0 |
| DCIBGDOO_4 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DCIBGDOO_1 | 799839 | 807094 | True | Siphoviridae | VOG0275, VOG0275, VOG1563, VOG1563, VOG9667, VOG6495, VOG0944, VOG8387, VOG4566 |
| DCIBGDOO_1 | 814219 | 835809 | False | Siphoviridae | VOG4841, VOG0713, VOG4581, VOG1886, VOG3607, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG1266, VOG9583, VOG0704, VOG4586, VOG0541, VOG5910, VOG0801, VOG4772, VOG4626, VOG4612, VOG6243, VOG4705 |
| DCIBGDOO_1 | 1580015 | 1610843 | False | Myoviridae | VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG0143, VOG4673, VOG1993, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862 |
| DCIBGDOO_1 | 2015063 | 2021603 | False | Siphoviridae | VOG3699, VOG0221, VOG5023, VOG0720, VOG4555, VOG4553 |
| DCIBGDOO_1 | 2947191 | 2976997 | True | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667, VOG4602, VOG0101, VOG0733, VOG0944, VOG5532, VOG0944 |
| DCIBGDOO_1 | 3657673 | 3679888 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG5615, VOG5535, VOG3390, VOG4599, VOG4605, VOG4545, VOG1323, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG4564, VOG0720, VOG10227, VOG0796, VOG0198 |
| DCIBGDOO_1 | 3686450 | 3693466 | False | Unknown | VOG1309, VOG4600, VOG1878, VOG4552, VOG0650, VOG9667, VOG9667, VOG8483 |
Phigaro v2.4.0 (Database: Jan. 2024 release)