safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
0	25	29	38.29

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
No hit found

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
No hit found

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
No hit found

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
KNAKEJLJ_1	1673110	1673703	STER_RS06030	0.0	Capsule	Immune modulation	100.00	100.00	WP_011226149
KNAKEJLJ_1	1546793	1548253	STER_RS05320	0.0	Capsule	Immune modulation	99.93	100.00	WP_011681177
KNAKEJLJ_1	1673110	1673703	STR_RS05880	0.0	Capsule	Immune modulation	99.83	100.00	WP_011226149
KNAKEJLJ_1	28595	29404	STU_RS16245	0.0	Capsule	Immune modulation	99.75	100.00	WP_002948020
KNAKEJLJ_1	1673703	1674572	rfbA	0.0	Capsule	Immune modulation	99.66	100.00	WP_011681286
KNAKEJLJ_1	1673110	1673703	STU_RS15260	0.0	Capsule	Immune modulation	99.66	100.00	WP_011226149
KNAKEJLJ_1	1428151	1429809	fbp54	0.0	FBPs	Adherence	99.64	100.00	WP_011227179
KNAKEJLJ_1	29388	30344	STU_RS16250	0.0	Capsule	Immune modulation	99.58	100.00	WP_011226328
KNAKEJLJ_1	584604	585443	cbpD	0.0	Autolysin	Exoenzyme	99.52	100.00	WP_011226733
KNAKEJLJ_1	24522	26306	STU_RS16230	0.0	Capsule	Immune modulation	99.44	100.00	WP_011226325
KNAKEJLJ_1	27390	28595	STU_RS16240	0.0	Capsule	Immune modulation	99.17	100.00	WP_011226326
KNAKEJLJ_1	36531	37382	rfbD	0.0	Capsule	Immune modulation	99.06	100.00	WP_014621820
KNAKEJLJ_1	1546061	1546792	STER_RS05315	0.0	Capsule	Immune modulation	98.91	100.00	WP_011681176
KNAKEJLJ_1	1709896	1710657	srtA	0.0	Sortase A	Exoenzyme	98.82	100.00	-
KNAKEJLJ_1	30341	31489	STU_RS16255	0.0	Capsule	Immune modulation	98.78	100.00	WP_041828290
KNAKEJLJ_1	1546061	1546792	STR_RS05260	0.0	Capsule	Immune modulation	98.50	100.00	WP_011227248
KNAKEJLJ_1	1546061	1546792	STU_RS14645	0.0	Capsule	Immune modulation	98.36	100.00	WP_011226048
KNAKEJLJ_1	1614135	1614928	cshA	0.0	Cell surface hydrophobicity proteins	Adherence	98.24	100.25	-
KNAKEJLJ_1	1534629	1536044	STER_RS05250	0.0	Capsule	Immune modulation	98.23	100.00	WP_011681165
KNAKEJLJ_1	1544610	1545350	STU_RS14635	0.0	Capsule	Immune modulation	98.11	100.00	WP_011226046
KNAKEJLJ_1	1544610	1545350	STER_RS05305	0.0	Capsule	Immune modulation	97.71	100.00	WP_011681174
KNAKEJLJ_1	1546793	1548253	STU_RS14650	0.0	Capsule	Immune modulation	97.40	100.21	WP_011226049
KNAKEJLJ_1	1544615	1545350	STR_RS05250	0.0	Capsule	Immune modulation	97.15	98.13	WP_011227246
KNAKEJLJ_1	1543186	1544553	STU_RS14630	0.0	Capsule	Immune modulation	97.00	100.00	WP_011226045
KNAKEJLJ_1	1546793	1548253	STR_RS05265	0.0	Capsule	Immune modulation	96.85	100.21	WP_041827043
KNAKEJLJ_1	1545360	1546052	STR_RS05255	0.0	Capsule	Immune modulation	95.38	100.00	WP_011227247
KNAKEJLJ_1	1545360	1546052	STU_RS14640	0.0	Capsule	Immune modulation	95.38	100.00	WP_011226047
KNAKEJLJ_1	26303	27370	STU_RS16235	0.0	Capsule	Immune modulation	95.27	101.14	WP_002948022
KNAKEJLJ_1	1543188	1544553	STER_RS05300	0.0	Capsule	Immune modulation	94.58	99.85	WP_011681173
KNAKEJLJ_1	1545360	1546052	STER_RS05310	0.0	Capsule	Immune modulation	90.48	100.00	WP_011681175
KNAKEJLJ_1	34154	35428	STU_RS16265	0.0	Capsule	Immune modulation	89.42	99.92	WP_011226331
KNAKEJLJ_1	1147447	1148750	eno	0.0	Streptococcal enolase	Exoenzyme	88.02	99.46	WP_000022832
KNAKEJLJ_1	322921	323931	plr/gapA	0.0	Streptococcal plasmin receptor/GAPDH	Adherence	86.54	99.41	WP_002986042
KNAKEJLJ_1	686640	687923	tig/ropA	0.0	Trigger factor	Stress survival	80.81	99.77	WP_000107753
KNAKEJLJ_1	1671635	1672681	rfbB	0.0	Capsule	Immune modulation	80.61	100.00	WP_009659423
KNAKEJLJ_1	1532226	1532809	SPD_RS01775	1.09e-151	Capsule	Immune modulation	83.59	96.20	WP_001819836

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
KNAKEJLJ_1	1713344	1714327	ldh(Sp_1220)	0.0	L-lactate dehydrogenase	94.82	100.00	AAK75326
KNAKEJLJ_1	1266719	1267792	MntE	0.0	manganese homeostasis	94.41	99.72	ABF32167
KNAKEJLJ_1	199939	202242	pfl2(Sp_0459)	0.0	formate acetyltransferase	92.97	99.22	AAK74619
KNAKEJLJ_1	1063809	1064777	spr0084	0.0	pathogenitcity related	92.57	98.48	AAK98888
KNAKEJLJ_1	1295039	1297285	metE	0.0	methionine biosynthesis enzymes	91.99	100.00	AAK74738
KNAKEJLJ_1	1783656	1785137	SPD_0310	0.0	UPF0371 protein SPD_0310	91.70	100.00	ABJ54480
KNAKEJLJ_1	604235	605233	recA	0.0	DNA recombination and repair	91.29	85.82	AAL00560
KNAKEJLJ_1	568121	569092	prsA2	0.0	ribose-phosphate pyrophosphokinase	90.12	100.00	ABF36968
KNAKEJLJ_1	85922	84771	potA	0.0	ABC transporter ATP-binding protein-spermidine/putrescine transport	89.06	99.74	AAL00050
KNAKEJLJ_1	50559	48571	uvrB	0.0	excinuclease ABC, B subunit	88.84	100.00	AE014259_2
KNAKEJLJ_1	1697145	1695415	ptsI	0.0	phosphoenolpyruvate-protein phosphotransferase	88.04	100.00	AAK34199
KNAKEJLJ_1	529957	528479	IMPDH	0.0	inosine-5'-monophosphate dehydrogenase	87.63	100.00	ADE32434
KNAKEJLJ_1	1147447	1148748	Eno	0.0	enolase	86.21	99.77	ABP90468
KNAKEJLJ_1	514768	512876	gidA	0.0	tRNA uridine 5-carboxymethylaminomethyl modification enzyme	86.05	98.90	ABP91129
KNAKEJLJ_1	1300210	1298501	PGM	0.0	phosphoglucomutase	83.89	99.65	ACH87041
KNAKEJLJ_1	366319	367329	pdh	0.0	pyruvate dehydrogenase	83.09	99.70	ABP91006
KNAKEJLJ_1	1451663	1450302	mnmE	0.0	central tRNA-modifying GTPase	82.24	99.56	ABP90420
KNAKEJLJ_1	416663	414771	PepO	0.0	endopeptidase	80.19	100.00	ABF32980
KNAKEJLJ_1	1133011	1133901	bglB	5.29e-178	putative phospho--glucosidase	88.55	99.66	AAK33487
KNAKEJLJ_1	1373054	1372116	SP_0676	2.37e-167	transcription facot	81.15	97.20	AAK74821
KNAKEJLJ_1	1297477	1298334	metF	1.54e-164	methionine biosynthesis enzymes	86.36	99.31	AAK74739
KNAKEJLJ_1	1586455	1585751	covR	4.41e-131	responder protein	85.53	100.00	AAK71319
KNAKEJLJ_1	1764681	1764010	rr01	3.02e-124	response regulator	83.93	99.56	CAB54566
KNAKEJLJ_1	1586455	1585751	walR	1.86e-121	DNA-binding response regulator	82.13	100.00	ANC99504
KNAKEJLJ_1	839668	840354	CovR	1.01e-117	serves to repress GAS virulence factor-encoding genes	80.79	100.00	ACE75886
KNAKEJLJ_1	1304164	1304832	CiaR	2.03e-117	two-component response regulator	84.31	99.55	AAK74935
KNAKEJLJ_1	1304164	1304835	ciaR	2.00e-115	two component system response regulator	82.14	99.12	AIK75708
KNAKEJLJ_1	605509	605883	SpxA2	1.16e-69	transcriptional regulator	91.20	94.70	ERL21461
KNAKEJLJ_1	1463025	1463426	SpxA1	3.79e-66	transcriptional regulator	80.60	100.00	ERL19273
KNAKEJLJ_1	688081	688581	RopE (M5005_Spy_1611)	4.95e-75	probable DNA-directed RNA polymerase subunit delta	81.44	87.43	AAK34603
KNAKEJLJ_1	199939	202236	PFL (B9H01_RS01125)	0.0	formate C-acetyltransferase	82.94	98.08	MYN69560
KNAKEJLJ_1	402203	401184	ilvC (SPD_0406)	0.0	ketol-acid reductoisomerase (NADP(+))	96.47	100.00	ABJ55451

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
KNAKEJLJ_1	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
No hit found