Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 0 | 26 | 32 | 41.23 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| No hit found | |||||||
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| JMCPFFIJ_26 | 63314 | 64282 | cbpD | 0.0 | Autolysin | Exoenzyme | 100.00 | 100.00 | WP_082302295 |
| JMCPFFIJ_22 | 95442 | 96035 | STR_RS05880 | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | WP_011226149 |
| JMCPFFIJ_25 | 76942 | 78201 | STER_RS07100 | 0.0 | Capsule | Immune modulation | 99.84 | 100.00 | WP_011681445 |
| JMCPFFIJ_22 | 95442 | 96035 | STER_RS06030 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| JMCPFFIJ_22 | 95442 | 96035 | STU_RS15260 | 0.0 | Capsule | Immune modulation | 99.83 | 100.00 | WP_011226149 |
| JMCPFFIJ_25 | 84205 | 85011 | STER_RS07060 | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | WP_011681437 |
| JMCPFFIJ_25 | 83264 | 84205 | STR_RS06905 | 0.0 | Capsule | Immune modulation | 99.68 | 100.00 | WP_011227430 |
| JMCPFFIJ_25 | 83264 | 84205 | STER_RS07065 | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | WP_011681438 |
| JMCPFFIJ_1 | 69820 | 71478 | fbp54 | 0.0 | FBPs | Adherence | 99.52 | 100.00 | WP_011227179 |
| JMCPFFIJ_25 | 84205 | 85011 | STR_RS06900 | 0.0 | Capsule | Immune modulation | 99.50 | 100.00 | WP_011227429 |
| JMCPFFIJ_25 | 78198 | 79175 | STER_RS07095 | 0.0 | Capsule | Immune modulation | 99.49 | 100.00 | WP_011681444 |
| JMCPFFIJ_25 | 82119 | 83267 | STR_RS06910 | 0.0 | Capsule | Immune modulation | 99.48 | 100.00 | WP_011227431 |
| JMCPFFIJ_25 | 82119 | 83267 | STER_RS07070 | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | WP_011681439 |
| JMCPFFIJ_25 | 79193 | 80161 | STER_RS07090 | 0.0 | Capsule | Immune modulation | 99.38 | 100.00 | WP_011681443 |
| JMCPFFIJ_25 | 87598 | 89343 | STER_RS07045 | 0.0 | Capsule | Immune modulation | 99.31 | 100.00 | WP_011681434 |
| JMCPFFIJ_22 | 59911 | 60672 | srtA | 0.0 | Sortase A | Exoenzyme | 99.21 | 100.00 | - |
| JMCPFFIJ_22 | 94573 | 95442 | rfbA | 0.0 | Capsule | Immune modulation | 99.19 | 100.00 | WP_011681286 |
| JMCPFFIJ_22 | 149902 | 150692 | cshA | 0.0 | Cell surface hydrophobicity proteins | Adherence | 98.74 | 100.00 | - |
| JMCPFFIJ_5 | 13979 | 14710 | STER_RS05315 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011681176 |
| JMCPFFIJ_5 | 13979 | 14710 | STU_RS14645 | 0.0 | Capsule | Immune modulation | 98.63 | 100.00 | WP_011226048 |
| JMCPFFIJ_5 | 13979 | 14710 | STR_RS05260 | 0.0 | Capsule | Immune modulation | 98.22 | 100.00 | WP_011227248 |
| JMCPFFIJ_25 | 81224 | 82000 | STER_RS07075 | 0.0 | Capsule | Immune modulation | 97.94 | 100.00 | WP_011681440 |
| JMCPFFIJ_25 | 87598 | 89343 | STR_RS06885 | 0.0 | Capsule | Immune modulation | 97.88 | 100.00 | WP_011227426 |
| JMCPFFIJ_25 | 80163 | 80878 | STER_RS07085 | 0.0 | Capsule | Immune modulation | 97.77 | 99.86 | WP_011681442 |
| JMCPFFIJ_5 | 12518 | 13978 | STER_RS05320 | 0.0 | Capsule | Immune modulation | 97.67 | 100.00 | WP_011681177 |
| JMCPFFIJ_5 | 16219 | 17280 | STU_RS14630 | 0.0 | Capsule | Immune modulation | 97.17 | 77.63 | WP_011226045 |
| JMCPFFIJ_5 | 12518 | 13978 | STU_RS14650 | 0.0 | Capsule | Immune modulation | 96.78 | 100.21 | WP_011226049 |
| JMCPFFIJ_25 | 75972 | 76823 | rfbD | 0.0 | Capsule | Immune modulation | 96.48 | 100.00 | WP_014621820 |
| JMCPFFIJ_5 | 15421 | 16156 | STR_RS05250 | 0.0 | Capsule | Immune modulation | 96.47 | 98.13 | WP_011227246 |
| JMCPFFIJ_5 | 15421 | 16158 | STER_RS05305 | 0.0 | Capsule | Immune modulation | 96.21 | 99.60 | WP_011681174 |
| JMCPFFIJ_5 | 12518 | 13978 | STR_RS05265 | 0.0 | Capsule | Immune modulation | 96.17 | 100.21 | WP_041827043 |
| JMCPFFIJ_5 | 15421 | 16158 | STU_RS14635 | 0.0 | Capsule | Immune modulation | 95.66 | 99.60 | WP_011226046 |
| JMCPFFIJ_5 | 16219 | 17280 | STER_RS05300 | 0.0 | Capsule | Immune modulation | 94.92 | 77.63 | WP_011681173 |
| JMCPFFIJ_5 | 14719 | 15411 | STU_RS14640 | 0.0 | Capsule | Immune modulation | 91.64 | 99.86 | WP_011226047 |
| JMCPFFIJ_5 | 14719 | 15411 | STR_RS05255 | 0.0 | Capsule | Immune modulation | 91.50 | 99.86 | WP_011227247 |
| JMCPFFIJ_11 | 95944 | 97247 | eno | 0.0 | Streptococcal enolase | Exoenzyme | 88.09 | 99.54 | WP_000022832 |
| JMCPFFIJ_31 | 14446 | 15456 | plr/gapA | 0.0 | Streptococcal plasmin receptor/GAPDH | Adherence | 86.92 | 99.51 | WP_002986042 |
| JMCPFFIJ_5 | 14719 | 15411 | STER_RS05310 | 0.0 | Capsule | Immune modulation | 86.78 | 99.57 | WP_011681175 |
| JMCPFFIJ_25 | 85011 | 86234 | SGO_RS05025 | 0.0 | Capsule | Immune modulation | 84.35 | 99.92 | WP_012000441 |
| JMCPFFIJ_25 | 84205 | 85011 | SGO_RS05020 | 0.0 | Capsule | Immune modulation | 83.15 | 100.00 | WP_012000440 |
| JMCPFFIJ_22 | 96464 | 97510 | rfbB | 0.0 | Capsule | Immune modulation | 80.80 | 100.00 | WP_009659423 |
| JMCPFFIJ_16 | 150 | 1433 | tig/ropA | 0.0 | Trigger factor | Stress survival | 80.58 | 99.77 | WP_000107753 |
| JMCPFFIJ_25 | 85011 | 85748 | STR_RS06895 | 0.0 | Capsule | Immune modulation | 97.97 | 61.35 | WP_011227428 |
| JMCPFFIJ_25 | 85011 | 85748 | STER_RS07055 | 0.0 | Capsule | Immune modulation | 97.70 | 61.35 | WP_011681436 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| JMCPFFIJ_22 | 57224 | 56241 | ldh(Sp_1220) | 0.0 | L-lactate dehydrogenase | 94.82 | 100.00 | AAK75326 |
| JMCPFFIJ_11 | 197397 | 198470 | MntE | 0.0 | manganese homeostasis | 94.41 | 99.72 | ABF32167 |
| JMCPFFIJ_11 | 12262 | 13230 | spr0084 | 0.0 | pathogenitcity related | 93.19 | 98.48 | AAK98888 |
| JMCPFFIJ_27 | 86207 | 88510 | pfl2(Sp_0459) | 0.0 | formate acetyltransferase | 93.10 | 99.22 | AAK74619 |
| JMCPFFIJ_11 | 217921 | 220167 | metE | 0.0 | methionine biosynthesis enzymes | 91.99 | 100.00 | AAK74738 |
| JMCPFFIJ_25 | 173619 | 172138 | SPD_0310 | 0.0 | UPF0371 protein SPD_0310 | 91.50 | 100.00 | ABJ54480 |
| JMCPFFIJ_9 | 2001 | 1003 | recA | 0.0 | DNA recombination and repair | 91.29 | 85.82 | AAL00560 |
| JMCPFFIJ_14 | 15450 | 14479 | prsA2 | 0.0 | ribose-phosphate pyrophosphokinase | 89.81 | 100.00 | ABF36968 |
| JMCPFFIJ_25 | 27566 | 28717 | potA | 0.0 | ABC transporter ATP-binding protein-spermidine/putrescine transport | 89.06 | 99.74 | AAL00050 |
| JMCPFFIJ_25 | 62934 | 64922 | uvrB | 0.0 | excinuclease ABC, B subunit | 88.99 | 100.00 | AE014259_2 |
| JMCPFFIJ_22 | 73418 | 75148 | ptsI | 0.0 | phosphoenolpyruvate-protein phosphotransferase | 88.04 | 100.00 | AAK34199 |
| JMCPFFIJ_28 | 27939 | 29417 | IMPDH | 0.0 | inosine-5'-monophosphate dehydrogenase | 87.63 | 100.00 | ADE32434 |
| JMCPFFIJ_28 | 43134 | 45026 | gidA | 0.0 | tRNA uridine 5-carboxymethylaminomethyl modification enzyme | 86.05 | 98.90 | ABP91129 |
| JMCPFFIJ_11 | 95944 | 97245 | Eno | 0.0 | enolase | 85.98 | 99.77 | ABP90468 |
| JMCPFFIJ_11 | 223092 | 221383 | PGM | 0.0 | phosphoglucomutase | 83.71 | 99.65 | ACH87041 |
| JMCPFFIJ_29 | 59785 | 58775 | pdh | 0.0 | pyruvate dehydrogenase | 83.09 | 99.70 | ABP91006 |
| JMCPFFIJ_30 | 48956 | 50317 | mnmE | 0.0 | central tRNA-modifying GTPase | 82.02 | 99.56 | ABP90420 |
| JMCPFFIJ_29 | 9520 | 11412 | PepO | 0.0 | endopeptidase | 80.19 | 100.00 | ABF32980 |
| JMCPFFIJ_11 | 81510 | 82400 | bglB | 1.64e-179 | putative phospho--glucosidase | 88.89 | 99.66 | AAK33487 |
| JMCPFFIJ_1 | 11797 | 10859 | SP_0676 | 3.16e-169 | transcription facot | 81.47 | 97.20 | AAK74821 |
| JMCPFFIJ_11 | 220359 | 221216 | metF | 1.07e-165 | methionine biosynthesis enzymes | 86.36 | 99.31 | AAK74739 |
| JMCPFFIJ_22 | 178339 | 179043 | covR | 2.73e-131 | responder protein | 84.68 | 100.00 | AAK71319 |
| JMCPFFIJ_22 | 4067 | 4738 | rr01 | 5.28e-127 | response regulator | 84.82 | 99.56 | CAB54566 |
| JMCPFFIJ_22 | 178339 | 179043 | walR | 3.27e-121 | DNA-binding response regulator | 81.28 | 100.00 | ANC99504 |
| JMCPFFIJ_2 | 58519 | 59205 | CovR | 3.73e-119 | serves to repress GAS virulence factor-encoding genes | 80.79 | 100.00 | ACE75886 |
| JMCPFFIJ_11 | 227045 | 227695 | CiaR | 3.47e-118 | two-component response regulator | 86.64 | 96.88 | AAK74935 |
| JMCPFFIJ_11 | 227045 | 227695 | ciaR | 1.47e-115 | two component system response regulator | 84.33 | 96.02 | AIK75708 |
| JMCPFFIJ_9 | 727 | 332 | SpxA2 | 4.75e-76 | transcriptional regulator | 90.91 | 100.00 | ERL21461 |
| JMCPFFIJ_6 | 13239 | 13526 | spr0479 | 2.97e-49 | essential gene | 86.46 | 98.97 | AAK99283 |
| JMCPFFIJ_26 | 22426 | 22172 | spr1327 | 2.02e-45 | essential gene | 95.29 | 100.00 | AAL00131 |
| JMCPFFIJ_28 | 76744 | 76481 | spr0175 | 4.37e-42 | pathogenitcity related | 84.09 | 100.00 | AAK98979 |
| JMCPFFIJ_28 | 75941 | 75651 | spr0177 | 2.57e-37 | essential gene | 81.00 | 98.02 | AAK98981 |
| JMCPFFIJ_3 | 62888 | 62388 | RopE (M5005_Spy_1611) | 1.67e-76 | probable DNA-directed RNA polymerase subunit delta | 81.44 | 87.43 | AAK34603 |
| JMCPFFIJ_27 | 86207 | 88504 | PFL (B9H01_RS01125) | 0.0 | formate C-acetyltransferase | 83.07 | 98.08 | MYN69560 |
| JMCPFFIJ_29 | 23923 | 24942 | ilvC (SPD_0406) | 0.0 | ketol-acid reductoisomerase (NADP(+)) | 96.47 | 100.00 | ABJ55451 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| JMCPFFIJ_24 | 1 | 885 | rep37 | 885 / 948 | 93.56 | AF177168 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| JMCPFFIJ_1 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_2 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_3 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_4 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_5 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_6 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_7 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_8 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_9 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_10 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_11 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_12 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_13 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_14 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_15 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_16 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_17 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_18 | 1.0 | 0.0 | 1.0 |
| JMCPFFIJ_20 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_21 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_22 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_23 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_24 | 1.0 | 0.0 | 1.0 |
| JMCPFFIJ_25 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_26 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_27 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_28 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_29 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_30 | 0.0 | 1.0 | 0.0 |
| JMCPFFIJ_31 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)