Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 7 | 17.83 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OECMMPNJ_91 | 736654 | 737508 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
| OECMMPNJ_91 | 717851 | 719053 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| OECMMPNJ_91 | 516635 | 517804 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| OECMMPNJ_112 | 454438 | 454755 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OECMMPNJ_112 | 454100 | 454438 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OECMMPNJ_122 | 368353 | 369273 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| OECMMPNJ_122 | 355019 | 356458 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.42 | JYFL01000006.1 |
| OECMMPNJ_122 | 305466 | 306059 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| OECMMPNJ_133 | 256309 | 258879 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
| OECMMPNJ_122 | 28789 | 30417 | vmlR | ARO:3004476 | vmlR | 98.52 | 99.09 | NC_000964.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OECMMPNJ_122 | 368353 | 369273 | mph(K) | Spiramycin, Telithromycin | 99.67 | 100.00 | NC_000964 |
| OECMMPNJ_102 | 60727 | 62103 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 98.84 | 100.00 | D12567 |
| OECMMPNJ_91 | 736654 | 737508 | aadK | Streptomycin | 98.25 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OECMMPNJ_122 | 368356 | 369273 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| OECMMPNJ_102 | 60727 | 62100 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.91 | 100.00 | WP_063856081.1 |
| OECMMPNJ_122 | 28789 | 30414 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.52 | 98.90 | WP_003234144.1 |
| OECMMPNJ_91 | 736657 | 737508 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| OECMMPNJ_102 | 64106 | 64624 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
| OECMMPNJ_91 | 213792 | 216389 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.71 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OECMMPNJ_102 | 543140 | 544321 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| OECMMPNJ_102 | 949377 | 950162 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| OECMMPNJ_102 | 953060 | 953998 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| OECMMPNJ_102 | 544804 | 545946 | capA | 0.0 | Capsule | Immune modulation | 99.39 | 100.00 | NP_391469 |
| OECMMPNJ_91 | 317063 | 317704 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| OECMMPNJ_102 | 544336 | 544785 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| OECMMPNJ_91 | 159439 | 161202 | dep/capD | 0.0 | Capsule | Immune modulation | 99.32 | 100.00 | NP_389723 |
| OECMMPNJ_102 | 954018 | 961154 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.31 | 100.00 | NP_391076 |
| OECMMPNJ_102 | 950188 | 951384 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| OECMMPNJ_102 | 951413 | 953032 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.33 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OECMMPNJ_144 | 31780 | 32916 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OECMMPNJ_177 | 11191 | 10322 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OECMMPNJ_112 | 765039 | 765815 | CodY | 7.83e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OECMMPNJ_102 | 698489 | 697920 | ClpP | 6.39e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| OECMMPNJ_112 | 277879 | 278271 | spxA1 | 6.15e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| OECMMPNJ_112 | 277879 | 278271 | spx | 3.67e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| OECMMPNJ_155 | 34712 | 34470 | SpoVG | 7.51e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| OECMMPNJ_122 | 89150 | 88953 | CspA | 2.02e-31 | cold shock protein | 90.91 | 100.00 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OECMMPNJ_91 | 0.0 | 1.0 | 0.0 |
| OECMMPNJ_102 | 0.0 | 1.0 | 0.0 |
| OECMMPNJ_112 | 0.0 | 1.0 | 0.0 |
| OECMMPNJ_122 | 0.0 | 1.0 | 0.0 |
| OECMMPNJ_133 | 0.0 | 1.0 | 0.0 |
| OECMMPNJ_144 | 0.0 | 1.0 | 0.0 |
| OECMMPNJ_155 | 0.0 | 1.0 | 0.0 |
| OECMMPNJ_166 | 0.0 | 1.0 | 0.0 |
| OECMMPNJ_177 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OECMMPNJ_91 | 6365 | 29367 | False | Siphoviridae | VOG4841, VOG4581, VOG1886, VOG4556, VOG4568, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641, VOG4918 |
| OECMMPNJ_112 | 398125 | 424612 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG4705 |
| OECMMPNJ_112 | 956479 | 958587 | False | Siphoviridae | VOG9667, VOG5353, VOG0181 |
| OECMMPNJ_133 | 6368 | 15480 | False | Siphoviridae | VOG0753, VOG0136, VOG4574, VOG1993, VOG2703, VOG9953, VOG3477, VOG3490, VOG3093 |
| OECMMPNJ_166 | 11745 | 16836 | False | Siphoviridae | VOG0275, VOG4602, VOG4662, VOG9667, VOG9667, VOG0650, VOG4552 |
| OECMMPNJ_166 | 23972 | 36533 | False | Siphoviridae | VOG3444, VOG0397, VOG0044, VOG9498, VOG0198, VOG7983, VOG0796, VOG10227, VOG0691, VOG0692, VOG9317, VOG4572, VOG2621, VOG0695, VOG0696, VOG0697, VOG6548 |
Phigaro v2.4.0 (Database: Jan. 2024 release)