Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 12 | 10 | 4 | 16.12 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OOAMKMIN_1 | 2662674 | 2663528 | aadK | ARO:3002627 | aadK | 97.89 | 100.00 | AL009126.1 |
| OOAMKMIN_1 | 2645841 | 2647043 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| OOAMKMIN_1 | 2452336 | 2453505 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| OOAMKMIN_1 | 1348561 | 1348878 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| OOAMKMIN_1 | 1348223 | 1348561 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| OOAMKMIN_1 | 874159 | 876729 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| OOAMKMIN_1 | 577382 | 579007 | vmlR | ARO:3004476 | vmlR | 96.86 | 98.90 | NC_000964.3 |
| OOAMKMIN_1 | 329857 | 330450 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| OOAMKMIN_1 | 279302 | 280735 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| OOAMKMIN_1 | 266487 | 267407 | mphK | ARO:3004541 | mphK | 99.01 | 100.00 | CP010314.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| OOAMKMIN_1 | 2662674 | 2663528 | aadK | Streptomycin | 98.71 | 100.00 | M26879 |
| OOAMKMIN_1 | 266487 | 267407 | mph(K) | Spiramycin, Telithromycin | 98.59 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| OOAMKMIN_1 | 266487 | 267395 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.01 | 99.02 | WP_003246254.1 |
| OOAMKMIN_1 | 2662677 | 2663528 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.89 | 100.00 | WP_003229862.1 |
| OOAMKMIN_1 | 577385 | 579007 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.86 | 98.90 | WP_003234144.1 |
| OOAMKMIN_1 | 4204033 | 4204551 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 94.22 | 100.00 | WP_003242546.1 |
| OOAMKMIN_1 | 2022501 | 2025098 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.60 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| OOAMKMIN_1 | 2255082 | 2255723 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.84 | 100.00 | NP_390062 |
| OOAMKMIN_1 | 3706201 | 3707382 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| OOAMKMIN_1 | 3704576 | 3705718 | capA | 0.0 | Capsule | Immune modulation | 99.74 | 100.00 | NP_391469 |
| OOAMKMIN_1 | 3282428 | 3283213 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| OOAMKMIN_1 | 3281206 | 3282402 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.50 | 100.00 | NP_391079 |
| OOAMKMIN_1 | 3278592 | 3279530 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| OOAMKMIN_1 | 3705737 | 3706186 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| OOAMKMIN_1 | 3271436 | 3278572 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.95 | 100.00 | NP_391076 |
| OOAMKMIN_1 | 1976768 | 1978531 | dep/capD | 0.0 | Capsule | Immune modulation | 98.92 | 100.00 | NP_389723 |
| OOAMKMIN_1 | 3279558 | 3281177 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.58 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| OOAMKMIN_1 | 132418 | 133554 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| OOAMKMIN_1 | 18603 | 19472 | pdxS | 3.15e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| OOAMKMIN_1 | 1660720 | 1661496 | CodY | 3.44e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| OOAMKMIN_1 | 3548163 | 3548732 | ClpP | 2.55e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| OOAMKMIN_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| OOAMKMIN_1 | 1289986 | 1317268 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| OOAMKMIN_1 | 2136085 | 2145146 | False | Myoviridae / Siphoviridae | VOG4724, VOG4577, VOG4692, VOG4551, VOG0025, VOG7627, VOG0322 |
| OOAMKMIN_1 | 2194327 | 2224746 | False | Siphoviridae | VOG10940, VOG7134, VOG9867, VOG6036, VOG5495, VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG8610, VOG7930, VOG0275, VOG4052, VOG4910, VOG3389, VOG5042, VOG9712, VOG4626, VOG3390, VOG4705, VOG4574 |
| OOAMKMIN_1 | 2831734 | 2855963 | False | Myoviridae | VOG6116, VOG6613, VOG4574, VOG0136, VOG0753, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG4553, VOG4555, VOG0720, VOG0796 |
| OOAMKMIN_1 | 3146752 | 3168123 | False | Siphoviridae | VOG0136, VOG0753, VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG6778, VOG4564, VOG0720, VOG10227, VOG0796 |
| OOAMKMIN_1 | 3175375 | 3181459 | False | Myoviridae | VOG0189, VOG5263, VOG1309, VOG4600, VOG1720, VOG4552 |
Phigaro v2.4.0 (Database: Jan. 2024 release)