Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 2 | 2 | 7 | 7.55 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCLBJMLG_5 | 56130 | 57179 | clbA | ARO:3002814 | clbA | 99.43 | 100.00 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| MCLBJMLG_5 | 56144 | 57179 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.80 | 98.67 | KR610408 |
| MCLBJMLG_55 | 133090 | 134466 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 86.78 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| MCLBJMLG_5 | 56133 | 57179 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 99.43 | 100.00 | WP_012116915.1 |
| MCLBJMLG_55 | 133090 | 134460 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.65 | 99.78 | WP_003242953.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| MCLBJMLG_4 | 19834 | 21015 | capB | 0.0 | Capsule | Immune modulation | 81.87 | 99.66 | NP_391471 |
| MCLBJMLG_4 | 21030 | 21479 | capC | 3.72e-115 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| MCLBJMLG_7 | 31826 | 32962 | tufA | 0.0 | elongation factor Tu | 86.81 | 95.69 | AHM69299 |
| MCLBJMLG_13 | 4458 | 5327 | pdxS | 1.36e-152 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| MCLBJMLG_22 | 160099 | 160875 | CodY | 5.99e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| MCLBJMLG_11 | 252865 | 253257 | spxA1 | 1.37e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| MCLBJMLG_5 | 70759 | 70562 | CspA | 4.69e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| MCLBJMLG_64 | 183911 | 184105 | CspD | 8.13e-28 | cold shock protein | 80.00 | 98.48 | CAC99957 |
| MCLBJMLG_64 | 183911 | 184108 | CspB | 1.69e-27 | cold shock protein | 80.30 | 100.00 | CAD00094 |
| MCLBJMLG_5 | 70759 | 70565 | CspR | 6.15e-27 | cold shock protein | 80.00 | 98.48 | AAO82613 |
| MCLBJMLG_1 | 1340 | 2212 | lipA (SAUSA300_0829) | 5.21e-163 | lipoic acid synthetase | 80.82 | 95.41 | ABD22039 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| MCLBJMLG_1 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_2 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_3 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_4 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_5 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_6 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_7 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_8 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_9 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_10 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_11 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_12 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_13 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_14 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_15 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_16 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_17 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_18 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_19 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_20 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_21 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_22 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_23 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_25 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_26 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_28 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_29 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_30 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_31 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_32 | 1.0 | 0.0 | 1.0 |
| MCLBJMLG_33 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_44 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_55 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_64 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_65 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_66 | 0.0 | 1.0 | 0.0 |
| MCLBJMLG_67 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| MCLBJMLG_1 | 150399 | 158473 | True | Siphoviridae | VOG7718, VOG3390, VOG4705, VOG8610, VOG6282, VOG3699, VOG0221, VOG7734, VOG0720 |
| MCLBJMLG_11 | 350503 | 377014 | False | Myoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG5660, VOG10055, VOG3890, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG4612, VOG3390, VOG4705, VOG0753 |
| MCLBJMLG_22 | 8182 | 17518 | False | Siphoviridae | VOG4705, VOG3390, VOG7718, VOG10904, VOG3549, VOG0585, VOG4341 |
| MCLBJMLG_64 | 12571 | 44465 | False | Siphoviridae | VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG1266, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG4841, VOG1638, VOG0275, VOG0198, VOG5447, VOG6581, VOG0397, VOG6108, VOG7788, VOG7454, VOG6587, VOG6931, VOG0189, VOG4566 |
Phigaro v2.4.0 (Database: Jan. 2024 release)