Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 9 | 0 | 0 | 9.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NBONGCMK_11 | 1358756 | 1359658 | cepA | ARO:3003559 | cepA | 99.67 | 100.00 | U05883.1 |
| NBONGCMK_11 | 1336502 | 1337707 | Mef(En2) | ARO:3004659 | Mef(En2) | 99.25 | 100.00 | AF251288.1 |
| NBONGCMK_44 | 375631 | 377556 | tet(Q) | ARO:3000191 | tet(Q) | 97.04 | 97.56 | Z21523.1 |
| NBONGCMK_44 | 360835 | 361635 | ErmF | ARO:3000498 | ErmF | 97.74 | 100.00 | M17124.1 |
| NBONGCMK_44 | 359390 | 360529 | tet(X1) | ARO:3005166 | tet(X1) | 99.72 | 105.57 | AJ311171.1 |
| NBONGCMK_44 | 358055 | 359191 | tet(X) | ARO:3000205 | tet(X) | 99.47 | 97.42 | M37699.1 |
| NBONGCMK_44 | 357016 | 357879 | aadS | ARO:3004683 | aadS | 100.00 | 100.00 | M72415.1 |
| NBONGCMK_2 | 26153 | 27118 | CfxA2 | ARO:3003002 | CfxA2 | 100.00 | 100.00 | AF118110.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NBONGCMK_44 | 375631 | 377556 | tet(Q) | Doxycycline, Tetracycline, Minocycline | 100.00 | 100.00 | X58717 |
| NBONGCMK_2 | 26153 | 27118 | cfxA3 | Ampicillin | 99.90 | 100.00 | AF472622 |
| NBONGCMK_2 | 26153 | 27118 | cfxA4 | Unknown Beta-lactam | 99.90 | 100.00 | AY769933 |
| NBONGCMK_2 | 26153 | 27118 | cfxA5 | Unknown Beta-lactam | 99.90 | 100.00 | AY769934 |
| NBONGCMK_2 | 26153 | 27118 | cfxA | Cefoxitin, Ampicillin | 99.90 | 100.00 | U38243 |
| NBONGCMK_11 | 1358756 | 1359658 | cepA | Unknown Beta-lactam | 99.89 | 100.00 | FR688022 |
| NBONGCMK_44 | 360835 | 361635 | erm(F) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.88 | 100.00 | M62487 |
| NBONGCMK_44 | 358055 | 359221 | tet(X) | Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.83 | 100.00 | M37699 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NBONGCMK_44 | 357019 | 357879 | aadS | aminoglycoside 6-adenylyltransferase AadS | EXACTX | 100.00 | 100.00 | WP_003013318.1 |
| NBONGCMK_11 | 1358756 | 1359655 | cepA | CepA family extended-spectrum class A beta-lactamase | EXACTX | 100.00 | 100.00 | WP_005816369.1 |
| NBONGCMK_2 | 26153 | 27115 | cfxA | CfxA family broad-spectrum class A beta-lactamase | EXACTX | 100.00 | 100.00 | WP_004339683.1 |
| NBONGCMK_11 | 1335968 | 1336477 | lnu(AN2) | lincosamide nucleotidyltransferase Lnu(AN2) | EXACTX | 100.00 | 100.00 | WP_004308783.1 |
| NBONGCMK_44 | 375631 | 377553 | tet(Q) | tetracycline resistance ribosomal protection protein Tet(Q) | EXACTX | 100.00 | 100.00 | WP_004293868.1 |
| NBONGCMK_44 | 358058 | 359221 | tet(X2) | tetracycline-inactivating monooxygenase Tet(X2) | EXACTX | 100.00 | 100.00 | WP_008651082.1 |
| NBONGCMK_44 | 359393 | 360529 | tet(X1) | tetracycline-inactivating monooxygenase Tet(X1) | BLASTX | 99.74 | 100.00 | WP_204376222.1 |
| NBONGCMK_44 | 360835 | 361632 | erm(F) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F) | BLASTX | 99.62 | 100.00 | WP_063844771.1 |
| NBONGCMK_11 | 1336505 | 1337707 | mef(En2) | macrolide efflux MFS transporter Mef(En2) | BLASTX | 99.25 | 100.00 | WP_063853729.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NBONGCMK_1 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_2 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_3 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_4 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_5 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_6 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_7 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_8 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_9 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_10 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_11 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_12 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_13 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_14 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_15 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_16 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_17 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_18 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_19 | 1.0 | 0.0 | 1.0 |
| NBONGCMK_20 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_21 | 1.0 | 0.0 | 1.0 |
| NBONGCMK_22 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_23 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_24 | 1.0 | 0.0 | 1.0 |
| NBONGCMK_25 | 1.0 | 0.0 | 1.0 |
| NBONGCMK_26 | 1.0 | 0.0 | 1.0 |
| NBONGCMK_30 | 1.0 | 0.0 | 1.0 |
| NBONGCMK_31 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_32 | 1.0 | 0.0 | 1.0 |
| NBONGCMK_33 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_44 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_49 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_50 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_51 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_52 | 0.0 | 1.0 | 0.0 |
| NBONGCMK_53 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NBONGCMK_11 | 1709711 | 1718848 | True | Siphoviridae | VOG2703, |
Phigaro v2.4.0 (Database: Jan. 2024 release)