Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 10 | 17.92 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EAMOFHHG_11 | 1110143 | 1111312 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| EAMOFHHG_44 | 140609 | 141202 | tmrB | ARO:3003059 | tmrB | 97.46 | 100.00 | AL009126.3 |
| EAMOFHHG_44 | 90052 | 91491 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.42 | JYFL01000006.1 |
| EAMOFHHG_44 | 77240 | 78160 | mphK | ARO:3004541 | mphK | 97.39 | 100.00 | CP010314.1 |
| EAMOFHHG_33 | 59216 | 60070 | aadK | ARO:3002627 | aadK | 98.59 | 100.00 | AL009126.1 |
| EAMOFHHG_11 | 41444 | 41761 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| EAMOFHHG_11 | 41106 | 41444 | ykkC | ARO:3003063 | ykkC | 98.21 | 100.00 | AL009126.1 |
| EAMOFHHG_33 | 39890 | 41092 | blt | ARO:3003006 | blt | 99.50 | 100.00 | L32599.1 |
| EAMOFHHG_46 | 28926 | 30569 | vmlR | ARO:3004476 | vmlR | 99.09 | 100.00 | NC_000964.3 |
| EAMOFHHG_45 | 27270 | 29840 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| EAMOFHHG_33 | 59216 | 60070 | aadK | Streptomycin | 98.83 | 100.00 | M26879 |
| EAMOFHHG_44 | 77240 | 78160 | mph(K) | Spiramycin, Telithromycin | 97.61 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| EAMOFHHG_46 | 28926 | 30566 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 99.09 | 100.00 | WP_003234144.1 |
| EAMOFHHG_33 | 59219 | 60070 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.59 | 100.00 | WP_003229862.1 |
| EAMOFHHG_44 | 77240 | 78157 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 97.39 | 100.00 | WP_003246254.1 |
| EAMOFHHG_11 | 773516 | 776113 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.29 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| EAMOFHHG_22 | 566738 | 567919 | capB | 0.0 | Capsule | Immune modulation | 99.75 | 100.00 | NP_391471 |
| EAMOFHHG_22 | 153388 | 154326 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.68 | 100.00 | NP_391077 |
| EAMOFHHG_22 | 157224 | 158009 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| EAMOFHHG_11 | 870992 | 871633 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| EAMOFHHG_22 | 146232 | 153368 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.30 | 100.00 | NP_391076 |
| EAMOFHHG_22 | 156002 | 157198 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| EAMOFHHG_22 | 565113 | 566255 | capA | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_391469 |
| EAMOFHHG_11 | 727831 | 729594 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| EAMOFHHG_22 | 566274 | 566723 | capC | 0.0 | Capsule | Immune modulation | 98.67 | 100.00 | NP_391470 |
| EAMOFHHG_22 | 154354 | 155973 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.09 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| EAMOFHHG_47 | 27978 | 26842 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| EAMOFHHG_9 | 1742 | 252 | Lqo | 0.0 | L-lactate-quinone oxidoreductases | 83.53 | 99.80 | AAW38622 |
| EAMOFHHG_5 | 11225 | 10356 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| EAMOFHHG_11 | 369028 | 369804 | CodY | 1.03e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| EAMOFHHG_2 | 26000 | 25314 | saeR | 1.80e-114 | response regulator | 83.84 | 100.00 | CAD89110 |
| EAMOFHHG_22 | 410761 | 411330 | ClpP | 4.42e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| EAMOFHHG_43 | 259862 | 260254 | spxA1 | 2.41e-70 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| EAMOFHHG_43 | 259862 | 260254 | spx | 1.44e-68 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| EAMOFHHG_1 | 20568 | 20810 | SpoVG | 7.49e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| EAMOFHHG_46 | 77238 | 77041 | CspA | 3.72e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| EAMOFHHG_43 | 17381 | 17187 | CspB | 3.48e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| EAMOFHHG_6 | 182 | 942 | rep5e | 762 / 867 | 87.53 | AP006719 |
| EAMOFHHG_6 | 9750 | 10191 | rep5d | 447 / 636 | 81.66 | AF051916 |
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| EAMOFHHG_1 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_2 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_3 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_4 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_5 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_6 | 1.0 | 0.0 | 1.0 |
| EAMOFHHG_7 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_8 | 1.0 | 0.0 | 1.0 |
| EAMOFHHG_9 | 1.0 | 0.0 | 1.0 |
| EAMOFHHG_11 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_12 | 1.0 | 0.0 | 1.0 |
| EAMOFHHG_22 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_33 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_42 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_43 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_44 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_45 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_46 | 0.0 | 1.0 | 0.0 |
| EAMOFHHG_47 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| EAMOFHHG_11 | 4165 | 10349 | False | Myoviridae | VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| EAMOFHHG_11 | 573130 | 596776 | False | Siphoviridae | VOG4581, VOG4544, VOG0202, VOG4556, VOG4573, VOG4553, VOG5361, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG5910, VOG6163, VOG0801, VOG4619, VOG4626, VOG3390, VOG4705, VOG4612, VOG3390, VOG7896 |
| EAMOFHHG_22 | 19876 | 44339 | False | Siphoviridae | VOG4574, VOG0136, VOG0753, VOG4728, VOG5535, VOG4599, VOG4605, VOG4545, VOG0725, VOG0799, VOG0724, VOG5660, VOG0816, VOG4623, VOG0815, VOG1125, VOG4553, VOG4555, VOG6778, VOG4564, VOG0720, VOG10227, VOG0796, VOG0198, VOG5574 |
| EAMOFHHG_43 | 362738 | 373064 | False | Siphoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749 |
Phigaro v2.4.0 (Database: Jan. 2024 release)