Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIDHJFFK_1 | 3917022 | 3917942 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| DIDHJFFK_1 | 3903694 | 3905127 | lmrB | ARO:3002813 | lmrB | 99.58 | 100.00 | JYFL01000006.1 |
| DIDHJFFK_1 | 3600446 | 3602089 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| DIDHJFFK_1 | 3311641 | 3314211 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| DIDHJFFK_1 | 2856556 | 2856894 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| DIDHJFFK_1 | 2856239 | 2856556 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| DIDHJFFK_1 | 1747231 | 1748400 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| DIDHJFFK_1 | 1524687 | 1525889 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| DIDHJFFK_1 | 1506325 | 1507179 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DIDHJFFK_1 | 3917022 | 3917942 | mph(K) | Spiramycin, Telithromycin | 99.35 | 100.00 | NC_000964 |
| DIDHJFFK_1 | 1506325 | 1507179 | aadK | Streptomycin | 98.95 | 100.00 | M26879 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DIDHJFFK_1 | 3917025 | 3917942 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| DIDHJFFK_1 | 1506325 | 1507176 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| DIDHJFFK_1 | 3600446 | 3602086 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| DIDHJFFK_1 | 38173 | 38691 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 93.64 | 100.00 | WP_003242546.1 |
| DIDHJFFK_1 | 2042024 | 2044621 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.65 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DIDHJFFK_1 | 943801 | 944586 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.75 | 100.00 | NP_391080 |
| DIDHJFFK_1 | 521916 | 523097 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| DIDHJFFK_1 | 1945791 | 1946432 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| DIDHJFFK_1 | 523112 | 523561 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| DIDHJFFK_1 | 944612 | 945808 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.33 | 100.00 | NP_391079 |
| DIDHJFFK_1 | 523580 | 524722 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| DIDHJFFK_1 | 2092223 | 2093986 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| DIDHJFFK_1 | 948442 | 955578 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.74 | 100.00 | NP_391076 |
| DIDHJFFK_1 | 947484 | 948422 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.40 | 100.00 | NP_391077 |
| DIDHJFFK_1 | 945837 | 947456 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.41 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DIDHJFFK_1 | 4039483 | 4038347 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| DIDHJFFK_1 | 4141344 | 4140475 | pdxS | 3.08e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DIDHJFFK_1 | 2545071 | 2544295 | CodY | 3.37e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DIDHJFFK_1 | 681213 | 680644 | ClpP | 2.50e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DIDHJFFK_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DIDHJFFK_1 | 1554491 | 1576706 | False | Myoviridae | VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4691, VOG4862 |
| DIDHJFFK_1 | 2257693 | 2266979 | False | Unknown | VOG4551, VOG0025, VOG7476, VOG0322, VOG6211, VOG8921, VOG0048 |
| DIDHJFFK_1 | 2308786 | 2332692 | False | Siphoviridae | VOG7134, VOG9867, VOG6036, VOG5495, VOG9219, VOG9713, VOG6940, VOG5534, VOG7718, VOG3390, VOG7895, VOG8610, VOG7930, VOG0275, VOG9312, VOG4910, VOG3389, VOG5042 |
| DIDHJFFK_1 | 2886439 | 2912754 | False | Myoviridae | VOG0753, VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)