Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 11 | 10 | 4 | 15.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IALFFNJO_1 | 4165630 | 4166550 | mphK | ARO:3004541 | mphK | 98.37 | 100.00 | CP010314.1 |
| IALFFNJO_1 | 4152304 | 4153737 | lmrB | ARO:3002813 | lmrB | 100.00 | 100.00 | JYFL01000006.1 |
| IALFFNJO_1 | 4102582 | 4103175 | tmrB | ARO:3003059 | tmrB | 97.97 | 100.00 | AL009126.3 |
| IALFFNJO_1 | 3849638 | 3851281 | vmlR | ARO:3004476 | vmlR | 96.71 | 100.00 | NC_000964.3 |
| IALFFNJO_1 | 3528791 | 3531361 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| IALFFNJO_1 | 3063584 | 3063922 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| IALFFNJO_1 | 3063267 | 3063584 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| IALFFNJO_1 | 1880838 | 1882007 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| IALFFNJO_1 | 1681641 | 1682843 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| IALFFNJO_1 | 1664718 | 1665572 | aadK | ARO:3002627 | aadK | 98.94 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| IALFFNJO_1 | 1664718 | 1665570 | aadK | Streptomycin | 98.83 | 99.77 | M26879 |
| IALFFNJO_1 | 4165630 | 4166550 | mph(K) | Spiramycin, Telithromycin | 98.15 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| IALFFNJO_1 | 1664718 | 1665569 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.94 | 100.00 | WP_003229862.1 |
| IALFFNJO_1 | 4165633 | 4166550 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.37 | 100.00 | WP_003246254.1 |
| IALFFNJO_1 | 3849638 | 3851278 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 96.71 | 100.00 | WP_003234144.1 |
| IALFFNJO_1 | 202643 | 203161 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| IALFFNJO_1 | 1092759 | 1093544 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.87 | 100.00 | NP_391080 |
| IALFFNJO_1 | 677134 | 678315 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| IALFFNJO_1 | 1096442 | 1097380 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.25 | 100.00 | NP_391077 |
| IALFFNJO_1 | 678798 | 679940 | capA | 0.0 | Capsule | Immune modulation | 99.12 | 100.00 | NP_391469 |
| IALFFNJO_1 | 678330 | 678779 | capC | 0.0 | Capsule | Immune modulation | 99.11 | 100.00 | NP_391470 |
| IALFFNJO_1 | 1093570 | 1094766 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.08 | 100.00 | NP_391079 |
| IALFFNJO_1 | 2394000 | 2395763 | dep/capD | 0.0 | Capsule | Immune modulation | 99.04 | 100.00 | NP_389723 |
| IALFFNJO_1 | 2119892 | 2120533 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| IALFFNJO_1 | 1097400 | 1104536 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.47 | 100.00 | NP_391076 |
| IALFFNJO_1 | 1094795 | 1096414 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.35 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| IALFFNJO_1 | 36155 | 35019 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| IALFFNJO_1 | 149894 | 149025 | pdxS | 3.17e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| IALFFNJO_1 | 2751726 | 2750950 | CodY | 3.46e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| IALFFNJO_1 | 841021 | 840452 | ClpP | 2.56e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| IALFFNJO_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| IALFFNJO_1 | 2163630 | 2180529 | False | Unknown | VOG9712, VOG5042, VOG3389, VOG4910, VOG9312, VOG0275, VOG7930, VOG8610, VOG4705, VOG7718 |
| IALFFNJO_1 | 2180820 | 2191690 | False | Siphoviridae | VOG5534, VOG6940, VOG9713, VOG9219, VOG5495, VOG6036, VOG7134, VOG10940, VOG5721 |
| IALFFNJO_1 | 2231777 | 2237332 | False | Unknown | VOG6211, VOG0322, VOG7476, VOG0025 |
| IALFFNJO_1 | 2526294 | 2548639 | False | Siphoviridae | VOG0641, VOG7896, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4573, VOG4556, VOG2935, VOG1886, VOG4581, VOG4841 |
| IALFFNJO_1 | 2555714 | 2560826 | False | Siphoviridae | VOG9600, VOG4566, VOG4552, VOG0181, VOG5353, VOG9667 |
| IALFFNJO_1 | 3093773 | 3121435 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
Phigaro v2.4.0 (Database: Jan. 2024 release)