Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ONBLCFJE_1 | 4045040 | 4045960 | mphK | ARO:3004541 | mphK | 99.02 | 100.00 | CP010314.1 |
| ONBLCFJE_1 | 4031711 | 4033144 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| ONBLCFJE_1 | 3981995 | 3982588 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| ONBLCFJE_1 | 3726700 | 3728343 | vmlR | ARO:3004476 | vmlR | 98.35 | 100.00 | NC_000964.3 |
| ONBLCFJE_1 | 3422358 | 3424928 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.88 | 100.00 | AL009126.3 |
| ONBLCFJE_1 | 2961395 | 2961733 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ONBLCFJE_1 | 2961078 | 2961395 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| ONBLCFJE_1 | 1826265 | 1827434 | bmr | ARO:3003007 | bmr | 99.23 | 100.00 | M33768.1 |
| ONBLCFJE_1 | 1596721 | 1597923 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| ONBLCFJE_1 | 1577900 | 1578754 | aadK | ARO:3002627 | aadK | 97.54 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ONBLCFJE_1 | 115102 | 116478 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 100.00 | 100.00 | X08034 |
| ONBLCFJE_1 | 1577900 | 1578752 | aadK | Streptomycin | 98.48 | 99.77 | M26879 |
| ONBLCFJE_1 | 4045040 | 4045960 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ONBLCFJE_1 | 115102 | 116475 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| ONBLCFJE_1 | 4045043 | 4045960 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 99.02 | 100.00 | WP_003246254.1 |
| ONBLCFJE_1 | 3726700 | 3728340 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.35 | 100.00 | WP_003234144.1 |
| ONBLCFJE_1 | 1577900 | 1578751 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 97.54 | 100.00 | WP_003229862.1 |
| ONBLCFJE_1 | 118477 | 118995 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 89.60 | 100.00 | WP_003242546.1 |
| ONBLCFJE_1 | 2261732 | 2264329 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.40 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ONBLCFJE_1 | 2025961 | 2026602 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
| ONBLCFJE_1 | 599776 | 600957 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| ONBLCFJE_1 | 1014495 | 1015280 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391080 |
| ONBLCFJE_1 | 1018178 | 1019116 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.36 | 100.00 | NP_391077 |
| ONBLCFJE_1 | 1015306 | 1016502 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.17 | 100.00 | NP_391079 |
| ONBLCFJE_1 | 2312651 | 2314414 | dep/capD | 0.0 | Capsule | Immune modulation | 99.15 | 100.00 | NP_389723 |
| ONBLCFJE_1 | 1019136 | 1026270 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.06 | 99.97 | NP_391076 |
| ONBLCFJE_1 | 601464 | 602606 | capA | 0.0 | Capsule | Immune modulation | 98.95 | 100.00 | NP_391469 |
| ONBLCFJE_1 | 1016531 | 1018150 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.52 | 100.00 | NP_391078 |
| ONBLCFJE_1 | 600972 | 601445 | capC | 0.0 | Capsule | Immune modulation | 93.67 | 105.11 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ONBLCFJE_3 | 27921 | 26785 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ONBLCFJE_1 | 71292 | 70423 | pdxS | 3.08e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ONBLCFJE_1 | 2651498 | 2650722 | CodY | 3.36e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ONBLCFJE_1 | 762335 | 761766 | ClpP | 2.49e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ONBLCFJE_1 | 0.0 | 1.0 | 0.0 |
| ONBLCFJE_2 | 1.0 | 0.0 | 1.0 |
| ONBLCFJE_3 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ONBLCFJE_1 | 1630938 | 1649250 | False | Myoviridae | VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| ONBLCFJE_1 | 2068758 | 2079897 | False | Unknown | VOG9712, VOG5042, VOG3389 |
| ONBLCFJE_1 | 2139991 | 2147294 | False | Unknown | VOG0048, VOG4908, VOG6211, VOG0322, VOG7476 |
| ONBLCFJE_1 | 2991543 | 3018448 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490 |
| ONBLCFJE_2 | 1585 | 18217 | False | Siphoviridae | VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG0221, VOG6862 |
| ONBLCFJE_2 | 43219 | 60940 | False | Siphoviridae | VOG0918, VOG1574, VOG5292, VOG4549, VOG2725, VOG3851, VOG9332, VOG2352, VOG4714, VOG4561, VOG1198, VOG0397, VOG2352, VOG11080 |
Phigaro v2.4.0 (Database: Jan. 2024 release)