safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
15	10	4	18.47

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
MOIKGNHB_1	2735268	2736122	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
MOIKGNHB_1	2716559	2717761	blt	ARO:3003006	blt	100.00	100.00	L32599.1
MOIKGNHB_1	2494240	2495409	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
MOIKGNHB_1	1376445	1376762	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
MOIKGNHB_1	1376107	1376445	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
MOIKGNHB_1	916372	918942	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
MOIKGNHB_1	604332	605975	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
MOIKGNHB_1	338754	339347	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
MOIKGNHB_1	288252	289685	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
MOIKGNHB_1	275437	276357	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
MOIKGNHB_3	12184	12921	ErmB	ARO:3000375	ErmB	98.78	98.79	AF242872.1
MOIKGNHB_3	733	2109	tet(L)	ARO:3000179	tet(L)	99.78	100.00	M11036.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
MOIKGNHB_1	2735268	2736122	aadK	Streptomycin	100.00	100.00	M26879
MOIKGNHB_3	12184	12921	erm(B)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	100.00	100.00	U18931
MOIKGNHB_1	275437	276357	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
MOIKGNHB_1	4186971	4188347	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
MOIKGNHB_1	2735271	2736122	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
MOIKGNHB_3	12187	12921	erm(B)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	EXACTX	100.00	100.00	WP_002292226.1
MOIKGNHB_1	275437	276354	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
MOIKGNHB_1	4184453	4184971	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
MOIKGNHB_1	4186974	4188347	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
MOIKGNHB_3	733	2106	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_001574277.1
MOIKGNHB_1	604335	605975	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
MOIKGNHB_1	2050926	2053520	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
MOIKGNHB_1	3697363	3698505	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
MOIKGNHB_1	3698988	3700169	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
MOIKGNHB_1	3698524	3698973	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
MOIKGNHB_1	2004268	2006031	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
MOIKGNHB_1	3291095	3291880	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
MOIKGNHB_1	3287259	3288197	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
MOIKGNHB_1	3289873	3291069	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
MOIKGNHB_1	3288225	3289844	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
MOIKGNHB_1	3280103	3287239	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
MOIKGNHB_1	2294894	2295535	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MOIKGNHB_1	132473	133609	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
MOIKGNHB_1	18665	19534	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
MOIKGNHB_1	1689709	1690485	CodY	5.93e-124	nutrient-responsive regulator	80.69	100.00	ACJ80679
MOIKGNHB_1	3545751	3546320	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
MOIKGNHB_3	96	482	rep34	387 / 633	100.00	M29725

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MOIKGNHB_1	0.0	1.0	0.0
MOIKGNHB_2	1.0	0.0	1.0
MOIKGNHB_3	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
MOIKGNHB_1	1320160	1346268	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
MOIKGNHB_1	2666630	2696874	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600
MOIKGNHB_2	8606	30941	False	Siphoviridae	VOG0221, VOG4581, VOG4544, VOG4556, VOG4568, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG5852, VOG6163, VOG4605, VOG4599, VOG9244, VOG10944, VOG1024
MOIKGNHB_2	50355	63453	False	Unknown	VOG0052, VOG11080, VOG2352, VOG0397, VOG1198, VOG4561, VOG4714, VOG2352, VOG3851, VOG2725

Phigaro v2.4.0 (Database: Jan. 2024 release)