Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 2 | 3 | 4.69 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AIHENMLD_1 | 395610 | 396656 | clbA | ARO:3002814 | clbA | 92.77 | 99.71 | CP006845.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| AIHENMLD_1 | 395610 | 396653 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.90 | 99.33 | KR610408 |
| AIHENMLD_1 | 2483941 | 2485317 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 87.07 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| AIHENMLD_1 | 395610 | 396653 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | BLASTX | 92.24 | 99.71 | WP_012116915.1 |
| AIHENMLD_1 | 2483947 | 2485317 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.43 | 99.78 | WP_003242953.1 |
| AIHENMLD_1 | 393341 | 393859 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 82.66 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| AIHENMLD_1 | 3533151 | 3534332 | capB | 0.0 | Capsule | Immune modulation | 82.04 | 99.66 | NP_391471 |
| AIHENMLD_1 | 3532687 | 3533136 | capC | 1.55e-113 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| AIHENMLD_1 | 4137169 | 4138305 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| AIHENMLD_1 | 4022391 | 4023260 | pdxS | 6.12e-150 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.48 | 98.31 | ACE61268 |
| AIHENMLD_1 | 1563763 | 1564539 | CodY | 3.45e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| AIHENMLD_1 | 0.0 | 1.0 | 0.0 |
| AIHENMLD_2 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| AIHENMLD_1 | 1397 | 16492 | False | Siphoviridae | VOG4553, VOG0204, VOG4589, VOG0207, VOG4586, VOG5852, VOG6163, VOG0801, VOG3462, VOG4619, VOG4856, VOG0804, VOG4705 |
| AIHENMLD_1 | 461054 | 504008 | False | Siphoviridae | VOG0275, VOG4602, VOG8483, VOG9667, VOG9667, VOG0806, VOG4322, VOG0650, VOG4552, VOG4600, VOG1309, VOG9093, VOG0189, VOG0044, VOG0536, VOG1336, VOG4658, VOG8569, VOG0397, VOG6581, VOG0102, VOG0044, VOG1137, VOG5447, VOG3631, VOG4972, VOG0796, VOG4544, VOG10227, VOG0720, VOG4564, VOG1300, VOG4555, VOG1329, VOG2621, VOG5872, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG0725, VOG1323, VOG4545, VOG4605, VOG4599, VOG3390, VOG5535, VOG0753, VOG0136, VOG4574 |
| AIHENMLD_1 | 756147 | 787429 | False | Siphoviridae | VOG5353, VOG0181, VOG4552, VOG3547, VOG4566, VOG9600, VOG0025, VOG1884, VOG5969, VOG0425, VOG5447, VOG0376, VOG2545, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG0207, VOG4586, VOG5852, VOG6163, VOG0801, VOG3462, VOG4619, VOG4856, VOG0804, VOG4705 |
| AIHENMLD_1 | 813161 | 816133 | False | Siphoviridae | VOG3390, VOG4705, VOG5221, VOG4824, VOG0602 |
| AIHENMLD_1 | 1170619 | 1196009 | False | Myoviridae | VOG9667, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG4545, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
| AIHENMLD_1 | 1629976 | 1653259 | False | Siphoviridae | VOG8537, VOG10230, VOG4660, VOG9712, VOG6023, VOG5042, VOG3389, VOG4910, VOG9312, VOG5250, VOG0275, VOG7930, VOG8610, VOG5008, VOG4705, VOG3390 |
| AIHENMLD_1 | 1714546 | 1720918 | False | Myoviridae | VOG0371, VOG6211, VOG0322, VOG7476, VOG0025, VOG4551 |
| AIHENMLD_1 | 2520475 | 2548136 | False | Siphoviridae | VOG10230, VOG0136, VOG5221, VOG4705, VOG0804, VOG4856, VOG4619, VOG3462, VOG0801, VOG6163, VOG5852, VOG4586, VOG0207, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841, VOG4556, VOG7884, VOG0275, VOG0198, VOG6242, VOG6517, VOG6581, VOG0397, VOG8569, VOG4658 |
| AIHENMLD_1 | 2668640 | 2695719 | False | Myoviridae | VOG10380, VOG10230, VOG0641, VOG4705, VOG3390, VOG7718, VOG4845, VOG4691, VOG4550, VOG0573, VOG1348, VOG1433, VOG1956, VOG6163, VOG1350, VOG4699, VOG1352, VOG1883, VOG1881, VOG1353, VOG4623, VOG4564, VOG1643, VOG4572, VOG4555, VOG0720, VOG10227, VOG0796, VOG4972, VOG3631, VOG5447 |
| AIHENMLD_1 | 2698084 | 2712357 | False | Siphoviridae | VOG0044, VOG0102, VOG6581, VOG0397, VOG8569, VOG4658, VOG1336, VOG0536, VOG0044, VOG0189, VOG9093, VOG1309, VOG4600, VOG4552, VOG4322, VOG0806, VOG9667, VOG9667 |
| AIHENMLD_1 | 3131278 | 3164968 | False | Siphoviridae | VOG4707, VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG5051, VOG4544, VOG4581, VOG0198, VOG4548, VOG0327, VOG4609, VOG10500, VOG0026, VOG1565, VOG0021, VOG1047, VOG7438 |
| AIHENMLD_1 | 3275083 | 3302167 | False | Siphoviridae | VOG5221, VOG4705, VOG0804, VOG4856, VOG4619, VOG3462, VOG0801, VOG6163, VOG5852, VOG4586, VOG0207, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841, VOG7884, VOG0275, VOG0198, VOG6242, VOG6581, VOG0397, VOG8569, VOG4658 |
| AIHENMLD_1 | 4160280 | 4174684 | False | Siphoviridae | VOG4602, VOG8520, VOG5353, VOG0181, VOG7438, VOG1047, VOG0021, VOG1565, VOG0026, VOG4567, VOG10500, VOG4609, VOG0327, VOG4548, VOG0198, VOG4841 |
Phigaro v2.4.0 (Database: Jan. 2024 release)