Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 3 | 0 | 0 | 3.00 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NKBGHIMF_45 | 213419 | 215338 | tet(W) | ARO:3000194 | tet(W) | 96.24 | 100.00 | AJ222769.3 |
| NKBGHIMF_18 | 2875 | 6438 | Bifidobacterium adolescentis rpoB mutants conferring resistance to rifampicin | ARO:3004480 | Bado_rpoB_RIF | 92.48 | 100.08 | NC_008618.1 |
| NKBGHIMF_37 | 752 | 1606 | ErmX | ARO:3000596 | ErmX | 90.14 | 100.00 | M36726.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| NKBGHIMF_37 | 844 | 1606 | erm(X) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 99.74 | 100.00 | U21300 |
| NKBGHIMF_45 | 213419 | 215338 | tet(W) | Doxycycline, Tetracycline, Minocycline | 97.81 | 100.00 | FN396364 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| NKBGHIMF_37 | 755 | 1606 | erm(X) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(X) | BLASTX | 99.65 | 100.00 | WP_011117480.1 |
| NKBGHIMF_45 | 213425 | 215338 | tet(W) | tetracycline resistance ribosomal protection protein Tet(W) | BLASTX | 99.53 | 99.69 | WP_063856422.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| No hit found | |||||||||
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| NKBGHIMF_1 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_2 | 1.0 | 0.0 | 1.0 |
| NKBGHIMF_3 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_4 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_5 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_6 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_7 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_8 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_9 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_10 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_11 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_12 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_13 | 1.0 | 0.0 | 1.0 |
| NKBGHIMF_15 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_16 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_17 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_18 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_19 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_20 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_21 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_22 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_23 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_25 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_26 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_27 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_28 | 1.0 | 0.0 | 1.0 |
| NKBGHIMF_30 | 1.0 | 0.0 | 1.0 |
| NKBGHIMF_31 | 1.0 | 0.0 | 1.0 |
| NKBGHIMF_32 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_34 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_37 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_38 | 1.0 | 0.0 | 1.0 |
| NKBGHIMF_45 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_56 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_67 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_74 | 0.0 | 1.0 | 0.0 |
| NKBGHIMF_75 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| NKBGHIMF_3 | 76603 | 96354 | False | Siphoviridae | VOG2038, VOG1154, VOG4544, VOG0720, VOG4576, VOG4555, VOG4553, VOG5504, VOG1581, VOG3409, VOG1159, VOG2639, VOG4557, VOG4582, VOG5793, VOG4569, VOG4599 |
| NKBGHIMF_7 | 41458 | 58265 | False | Siphoviridae | VOG3556, VOG2090, VOG8440, VOG0731, VOG6371, VOG2021, VOG10593, VOG5088, VOG11003, VOG0982 |
| NKBGHIMF_23 | 415607 | 420917 | False | Siphoviridae | VOG4641, VOG2577, VOG1837, VOG4602, VOG6495, VOG1563 |
| NKBGHIMF_23 | 427415 | 439995 | False | Siphoviridae | VOG7780, VOG2038, VOG0296, VOG4618, VOG1146, VOG4544, VOG4556, VOG4568, VOG4553, VOG1210, VOG1159, VOG4621 |
| NKBGHIMF_45 | 218782 | 230094 | False | Myoviridae | VOG4548, VOG1931, VOG4548, VOG3594, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)