Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 5 | 1 | 3 | 5.92 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HHEMLNNI_1 | 328383 | 329228 | Erm(34) | ARO:3000600 | Erm(34) | 96.09 | 100.00 | AY234334.1 |
| HHEMLNNI_23 | 179171 | 179818 | Alkalihalobacillus clausii chloramphenicol acetyltransferase | ARO:3004452 | Acla_ACT_CHL | 87.26 | 94.30 | AY238971.1 |
| HHEMLNNI_45 | 141092 | 142144 | clbC | ARO:3002816 | clbC | 99.14 | 100.00 | AP006627.1 |
| HHEMLNNI_34 | 63977 | 64747 | ANT(4')-Ib | ARO:3003905 | ANT(4')-Ib | 98.44 | 100.00 | EF540343.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HHEMLNNI_34 | 63977 | 64747 | ant(4')-Ib | Amikacin, Tobramycin, Isepamicin, Dibekacin | 99.22 | 100.00 | AJ506108 |
| HHEMLNNI_23 | 179173 | 179849 | cat | Chloramphenicol | 96.79 | 98.40 | AY238971 |
| HHEMLNNI_1 | 328390 | 329228 | erm(34) | Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S | 96.07 | 99.17 | AY234334 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HHEMLNNI_34 | 60662 | 61564 | blaBCL | BCL family class A beta-lactamase | BLASTX | 99.34 | 100.00 | WP_065825670.1 |
| HHEMLNNI_45 | 141095 | 142144 | clbB | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbB | BLASTX | 99.14 | 100.00 | WP_011245929.1 |
| HHEMLNNI_23 | 179171 | 179815 | catA | type A-10 chloramphenicol O-acetyltransferase | BLASTX | 98.60 | 100.00 | WP_035203840.1 |
| HHEMLNNI_34 | 63980 | 64747 | aadD2 | aminoglycoside O-nucleotidyltransferase ANT(4')-Ib | BLASTX | 98.44 | 100.00 | WP_035202393.1 |
| HHEMLNNI_1 | 328389 | 329228 | erm(34) | 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(34) | BLASTX | 96.43 | 99.64 | WP_063844504.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HHEMLNNI_12 | 378612 | 379262 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.85 | 100.00 | WP_011247375 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HHEMLNNI_3 | 34936 | 33800 | tufA | 0.0 | elongation factor Tu | 87.33 | 95.69 | AHM69299 |
| HHEMLNNI_2 | 42561 | 42319 | SpoVG | 2.56e-37 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| HHEMLNNI_52 | 104832 | 105026 | CspA | 6.16e-30 | cold shock protein | 86.15 | 98.48 | CAA62903 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HHEMLNNI_1 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_2 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_3 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_4 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_5 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_6 | 1.0 | 0.0 | 1.0 |
| HHEMLNNI_8 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_12 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_23 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_34 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_45 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_51 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_52 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_53 | 0.0 | 1.0 | 0.0 |
| HHEMLNNI_54 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| No hit found | |||||
Phigaro v2.4.0 (Database: Jan. 2024 release)