safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
ELIPLHKN_1	3935373	3937943	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
ELIPLHKN_1	3477882	3478220	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
ELIPLHKN_1	3477565	3477882	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
ELIPLHKN_1	2358916	2360085	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
ELIPLHKN_1	2136564	2137766	blt	ARO:3003006	blt	100.00	100.00	L32599.1
ELIPLHKN_1	2118203	2119057	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
ELIPLHKN_1	362524	363444	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1
ELIPLHKN_1	349196	350629	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
ELIPLHKN_1	299533	300126	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
ELIPLHKN_1	32904	34547	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
ELIPLHKN_1	2118203	2119057	aadK	Streptomycin	100.00	100.00	M26879
ELIPLHKN_1	362524	363444	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
ELIPLHKN_1	665674	667050	tet(L)	Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
ELIPLHKN_1	2118203	2119054	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
ELIPLHKN_1	362527	363444	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
ELIPLHKN_1	669050	669568	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
ELIPLHKN_1	665674	667047	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
ELIPLHKN_1	32904	34544	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
ELIPLHKN_1	2800806	2803400	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
ELIPLHKN_1	1155769	1156911	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
ELIPLHKN_1	1154105	1155286	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
ELIPLHKN_1	1155301	1155750	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
ELIPLHKN_1	2848295	2850058	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
ELIPLHKN_1	1562461	1563246	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
ELIPLHKN_1	1566144	1567082	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
ELIPLHKN_1	1563272	1564468	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
ELIPLHKN_1	1564497	1566116	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
ELIPLHKN_1	1567102	1574238	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
ELIPLHKN_1	2558791	2559432	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
ELIPLHKN_1	506330	505194	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
ELIPLHKN_1	620075	619206	pdxS	3.13e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
ELIPLHKN_1	3164618	3163842	CodY	3.42e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
ELIPLHKN_1	1308523	1307954	ClpP	2.54e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
ELIPLHKN_1	0.0	1.0	0.0
ELIPLHKN_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
ELIPLHKN_1	2157318	2187695	False	Myoviridae	VOG4600, VOG1309, VOG11060, VOG0189, VOG4693, VOG9587, VOG4673, VOG0796, VOG10227, VOG4773, VOG4688, VOG4685, VOG0749, VOG4589, VOG4870, VOG10055, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718
ELIPLHKN_1	3508059	3534167	False	Myoviridae	VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667
ELIPLHKN_2	14779	27877	False	Unknown	VOG4549, VOG2725, VOG3851, VOG2352, VOG4714, VOG4561, VOG1198, VOG0397, VOG2352, VOG11080
ELIPLHKN_2	47291	70939	False	Siphoviridae	VOG3616, VOG1024, VOG10944, VOG9244, VOG4599, VOG4605, VOG6163, VOG5852, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG4553, VOG4568, VOG4556, VOG4544, VOG4581, VOG0221

Phigaro v2.4.0 (Database: Jan. 2024 release)