Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 4 | 2 | 3 | 5.39 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| HEMLNNMD_1 | 3067643 | 3068692 | cfr(B) | Chloramphenicol, Florfenicol, Clindamycin, Lincomycin, Linezolid, Dalfopristin, Pristinamycin IIA, Virginiamycin M, Tiamulin | 88.77 | 99.90 | KR610408 |
| HEMLNNMD_1 | 1117810 | 1119186 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 87.15 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| HEMLNNMD_1 | 3067643 | 3068689 | clbA | 23S rRNA (adenine(2503)-C(8))-methyltransferase ClbA | INTERNAL_STOP | 92.55 | 100.00 | WP_012116915.1 |
| HEMLNNMD_1 | 1117816 | 1119186 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 86.87 | 99.78 | WP_003242953.1 |
| HEMLNNMD_1 | 3065381 | 3065899 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 85.55 | 100.00 | WP_003242546.1 |
| HEMLNNMD_1 | 506778 | 509375 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.17 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| HEMLNNMD_1 | 2062231 | 2063412 | capB | 0.0 | Capsule | Immune modulation | 82.12 | 99.66 | NP_391471 |
| HEMLNNMD_1 | 2061767 | 2062216 | capC | 1.49e-113 | Capsule | Immune modulation | 83.59 | 99.78 | NP_391470 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| HEMLNNMD_1 | 2675029 | 2676165 | tufA | 0.0 | elongation factor Tu | 86.54 | 95.69 | AHM69299 |
| HEMLNNMD_1 | 2560184 | 2561053 | pdxS | 1.58e-150 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 84.83 | 98.31 | ACE61268 |
| HEMLNNMD_1 | 299093 | 299869 | CodY | 3.32e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| HEMLNNMD_1 | 0.0 | 1.0 | 0.0 |
| HEMLNNMD_2 | 1.0 | 0.0 | 1.0 |
| HEMLNNMD_3 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| HEMLNNMD_1 | 1220387 | 1248550 | False | Siphoviridae | VOG0641, VOG7896, VOG3390, VOG4612, VOG4660, VOG4660, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG1266, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG4841, VOG2545, VOG0376, VOG0198, VOG7372, VOG2752 |
| HEMLNNMD_1 | 1375048 | 1406899 | False | Siphoviridae | VOG0047, VOG10230, VOG0136, VOG5221, VOG4705, VOG0804, VOG4856, VOG4619, VOG3462, VOG0801, VOG6163, VOG5852, VOG4586, VOG0207, VOG4589, VOG0204, VOG4553, VOG4573, VOG4556, VOG4544, VOG4581, VOG4841, VOG3254, VOG7888, VOG2545, VOG0376, VOG5447, VOG1884, VOG0025, VOG9600, VOG4566, VOG3547, VOG4552 |
| HEMLNNMD_1 | 3131985 | 3142662 | False | Siphoviridae | VOG6513, VOG0275, VOG4602, VOG1561, VOG9667, VOG11075, VOG0650, VOG4552, VOG4600, VOG1309 |
| HEMLNNMD_1 | 3152494 | 3174685 | False | Siphoviridae | VOG4972, VOG8642, VOG0796, VOG0720, VOG4564, VOG4555, VOG4553, VOG1125, VOG3649, VOG4623, VOG0816, VOG5660, VOG0724, VOG0799, VOG2621, VOG0725, VOG1323, VOG4545, VOG4605, VOG4599, VOG5535, VOG0641 |
| HEMLNNMD_1 | 3462411 | 3468517 | False | Siphoviridae | VOG5753, VOG10937, VOG6497, VOG0026 |
| HEMLNNMD_1 | 3480425 | 3482869 | False | Unknown | VOG4567, VOG0052 |
| HEMLNNMD_1 | 3491084 | 3523267 | False | Siphoviridae | VOG6430, VOG0531, VOG4841, VOG4581, VOG4544, VOG4556, VOG4573, VOG4553, VOG0204, VOG4589, VOG0539, VOG0704, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0753, VOG4574, VOG0602, VOG8440, VOG6116, VOG5216, VOG0275, VOG1563, VOG6495 |
| HEMLNNMD_1 | 3531326 | 3555531 | False | Siphoviridae | VOG6242, VOG0198, VOG0275, VOG7884, VOG0531, VOG0519, VOG4544, VOG4556, VOG4573, VOG4553, VOG3435, VOG0204, VOG4589, VOG0704, VOG0539, VOG4586, VOG0541, VOG6163, VOG0801, VOG4619, VOG4660, VOG4612, VOG3390, VOG4705, VOG0641 |
| HEMLNNMD_1 | 3907421 | 3932785 | False | Myoviridae | VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG0749, VOG10055, VOG3890, VOG3891, VOG3892, VOG4545, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718 |
Phigaro v2.4.0 (Database: Jan. 2024 release)