Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 8 | 18.25 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BIOIEKCG_23 | 457855 | 458709 | aadK | ARO:3002627 | aadK | 100.00 | 100.00 | AL009126.1 |
| BIOIEKCG_23 | 439146 | 440348 | blt | ARO:3003006 | blt | 100.00 | 100.00 | L32599.1 |
| BIOIEKCG_12 | 425257 | 425574 | ykkD | ARO:3003064 | ykkD | 100.00 | 100.00 | AL009126.1 |
| BIOIEKCG_12 | 424919 | 425257 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| BIOIEKCG_56 | 251998 | 252918 | mphK | ARO:3004541 | mphK | 100.00 | 100.00 | CP010314.1 |
| BIOIEKCG_56 | 238664 | 240103 | lmrB | ARO:3002813 | lmrB | 99.79 | 100.42 | JYFL01000006.1 |
| BIOIEKCG_23 | 216827 | 217996 | bmr | ARO:3003007 | bmr | 99.74 | 100.00 | M33768.1 |
| BIOIEKCG_56 | 189007 | 189600 | tmrB | ARO:3003059 | tmrB | 100.00 | 100.00 | AL009126.3 |
| BIOIEKCG_89 | 47320 | 48963 | vmlR | ARO:3004476 | vmlR | 100.00 | 100.00 | NC_000964.3 |
| BIOIEKCG_78 | 27304 | 29874 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 100.00 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| BIOIEKCG_23 | 457855 | 458709 | aadK | Streptomycin | 100.00 | 100.00 | M26879 |
| BIOIEKCG_56 | 251998 | 252918 | mph(K) | Spiramycin, Telithromycin | 100.00 | 100.00 | NC_000964 |
| BIOIEKCG_45 | 331276 | 332652 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 100.00 | 100.00 | X08034 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| BIOIEKCG_23 | 457858 | 458709 | aadK | aminoglycoside 6-adenylyltransferase AadK | EXACTX | 100.00 | 100.00 | WP_003229862.1 |
| BIOIEKCG_56 | 252001 | 252918 | mphK | macrolide 2'-phosphotransferase MphK | EXACTX | 100.00 | 100.00 | WP_003246254.1 |
| BIOIEKCG_45 | 328758 | 329276 | satA | streptothricin N-acetyltransferase SatA | EXACTX | 100.00 | 100.00 | WP_003242546.1 |
| BIOIEKCG_45 | 331279 | 332652 | tet(L) | tetracycline efflux MFS transporter Tet(L) | EXACTX | 100.00 | 100.00 | WP_003242953.1 |
| BIOIEKCG_89 | 47323 | 48963 | vmlR | ABC-F type ribosomal protection protein VmlR | EXACTX | 100.00 | 100.00 | WP_003234144.1 |
| BIOIEKCG_67 | 150396 | 152990 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 81.15 | 99.88 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| BIOIEKCG_34 | 519106 | 520248 | capA | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391469 |
| BIOIEKCG_34 | 520731 | 521912 | capB | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391471 |
| BIOIEKCG_34 | 520267 | 520716 | capC | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_391470 |
| BIOIEKCG_67 | 103738 | 105501 | dep/capD | 0.0 | Capsule | Immune modulation | 100.00 | 100.00 | NP_389723 |
| BIOIEKCG_34 | 112771 | 113556 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391080 |
| BIOIEKCG_34 | 108935 | 109873 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391077 |
| BIOIEKCG_34 | 111549 | 112745 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391079 |
| BIOIEKCG_34 | 109901 | 111520 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391078 |
| BIOIEKCG_34 | 101779 | 108915 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 100.00 | 100.00 | NP_391076 |
| BIOIEKCG_23 | 17480 | 18121 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 100.00 | 100.00 | NP_390062 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| BIOIEKCG_100 | 31693 | 32829 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| BIOIEKCG_5 | 11169 | 10300 | pdxS | 1.11e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| BIOIEKCG_12 | 738521 | 739297 | CodY | 7.70e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| BIOIEKCG_34 | 367494 | 368063 | ClpP | 4.07e-99 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| BIOIEKCG_12 | 276096 | 276488 | spxA1 | 2.28e-69 | redox-responsive transcription factor | 83.21 | 100.00 | AAF21893 |
| BIOIEKCG_2 | 20818 | 21060 | SpoVG | 7.53e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| BIOIEKCG_89 | 1848 | 2045 | CspA | 3.37e-32 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| BIOIEKCG_23 | 30083 | 30277 | CspD | 5.50e-28 | cold shock protein | 81.54 | 98.48 | CAC99957 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| BIOIEKCG_2 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_3 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_4 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_5 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_6 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_7 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_8 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_10 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_11 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_12 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_13 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_14 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_15 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_16 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_17 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_19 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_20 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_21 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_22 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_23 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_24 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_25 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_26 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_27 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_28 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_29 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_30 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_31 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_32 | 1.0 | 0.0 | 1.0 |
| BIOIEKCG_34 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_45 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_56 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_67 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_78 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_89 | 0.0 | 1.0 | 0.0 |
| BIOIEKCG_100 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| BIOIEKCG_12 | 368972 | 395080 | False | Myoviridae | VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| BIOIEKCG_23 | 389217 | 419461 | False | Myoviridae | VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600 |
Phigaro v2.4.0 (Database: Jan. 2024 release)