safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

Probiotic potential risk score

ARGs	VFs	PGs	PPRS
13	0	1	13.04

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Antibiotic resistance genes

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
MAJCONDE_10	1188733	1189938	Mef(En2)	ARO:3004659	Mef(En2)	99.50	100.00	AF251288.1
MAJCONDE_10	1065053	1066258	Mef(En2)	ARO:3004659	Mef(En2)	99.50	100.00	AF251288.1
MAJCONDE_10	693523	695448	tet(Q)	ARO:3000191	tet(Q)	97.04	97.56	Z21523.1
MAJCONDE_1	665186	667111	tet(Q)	ARO:3000191	tet(Q)	97.66	97.56	Z21523.1
MAJCONDE_1	650457	651257	ErmF	ARO:3000498	ErmF	97.37	100.00	M17124.1
MAJCONDE_1	649012	650151	tet(X1)	ARO:3005166	tet(X1)	99.72	105.57	AJ311171.1
MAJCONDE_1	647677	648813	tet(X)	ARO:3000205	tet(X)	99.21	97.42	M37699.1
MAJCONDE_1	646638	647501	aadS	ARO:3004683	aadS	100.00	100.00	M72415.1
MAJCONDE_7	50503	51240	ErmB	ARO:3000375	ErmB	97.96	98.79	AF242872.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
MAJCONDE_10	693523	695448	tet(Q)	Doxycycline, Tetracycline, Minocycline	99.95	100.00	X58717
MAJCONDE_1	650457	651257	erm(F)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.75	100.00	M62487
MAJCONDE_1	647677	648843	tet(X)	Doxycycline, Tetracycline, Minocycline, Tigecycline	99.74	100.00	M37699
MAJCONDE_7	50503	51240	erm(B)	Erythromycin, Lincomycin, Clindamycin, Quinupristin, Pristinamycin IA, Virginiamycin S	99.73	100.00	U18931

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
MAJCONDE_1	646641	647501	aadS	aminoglycoside 6-adenylyltransferase AadS	EXACTX	100.00	100.00	WP_003013318.1
MAJCONDE_11	405786	406691	blaMUN-1	MUN family extended-spectrum class A beta-lactamase MUN-1	ALLELEX	100.00	100.00	WP_206340447.1
MAJCONDE_12	880649	881554	blaMUN-1	MUN family extended-spectrum class A beta-lactamase MUN-1	ALLELEX	100.00	100.00	WP_206340447.1
MAJCONDE_10	1066283	1066792	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
MAJCONDE_10	1189963	1190472	lnu(AN2)	lincosamide nucleotidyltransferase Lnu(AN2)	EXACTX	100.00	100.00	WP_004308783.1
MAJCONDE_10	693523	695445	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	EXACTX	100.00	100.00	WP_004293868.1
MAJCONDE_1	649015	650151	tet(X1)	tetracycline-inactivating monooxygenase Tet(X1)	BLASTX	99.74	100.00	WP_204376222.1
MAJCONDE_1	647680	648843	tet(X2)	tetracycline-inactivating monooxygenase Tet(X2)	BLASTX	99.74	100.00	WP_008651082.1
MAJCONDE_10	1065053	1066255	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.50	100.00	WP_063853729.1
MAJCONDE_10	1188733	1189935	mef(En2)	macrolide efflux MFS transporter Mef(En2)	BLASTX	99.50	100.00	WP_063853729.1
MAJCONDE_1	650457	651254	erm(F)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(F)	BLASTX	99.25	100.00	WP_063844771.1
MAJCONDE_7	50503	51237	erm(B)	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	BLASTX	99.18	100.00	WP_002292226.1
MAJCONDE_1	665186	667108	tet(Q)	tetracycline resistance ribosomal protection protein Tet(Q)	BLASTX	97.66	100.00	WP_002560998.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence factors

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	e-value	VF name	VF category	Identity	Coverage	Accession no.
No hit found

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

Pathogenic genes

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
MAJCONDE_12	310485	312065	GroEL	0.0	folding of newly synthesized proteins, preventing misfolding and aggregation	80.64	96.70	BAA04222

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

Plasmid

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
MAJCONDE_1	0.0	1.0	0.0
MAJCONDE_2	1.0	0.0	1.0
MAJCONDE_3	1.0	0.0	1.0
MAJCONDE_4	0.0	1.0	0.0
MAJCONDE_5	1.0	0.0	1.0
MAJCONDE_6	1.0	0.0	1.0
MAJCONDE_7	0.0	1.0	0.0
MAJCONDE_8	0.0	1.0	0.0
MAJCONDE_9	0.0	1.0	0.0
MAJCONDE_10	0.0	1.0	0.0
MAJCONDE_11	0.0	1.0	0.0
MAJCONDE_12	0.0	1.0	0.0
MAJCONDE_13	0.0	1.0	0.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Prophage

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
MAJCONDE_7	20522	30318	True	Siphoviridae	VOG0158,
MAJCONDE_10	230931	236457	True	Unknown	VOG0275,
MAJCONDE_10	304968	314719	True	Unknown	VOG8253,
MAJCONDE_12	463948	475094	False	Unknown	VOG3992,