safety

PPRS ARGs VFs PGs Plasmid Prophage IS elements

ARGs	VFs	PGs	PPRS
13	10	4	16.88

PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).

Comprehensive Antibiotic Resistance Database (CARD)

Query ID	Begin	End	ARO name	ARO accession	CARD name	Identity	Coverage	Accession no.
CKPLAKGK_1	2735277	2736131	aadK	ARO:3002627	aadK	100.00	100.00	AL009126.1
CKPLAKGK_1	2716568	2717770	blt	ARO:3003006	blt	100.00	100.00	L32599.1
CKPLAKGK_1	2494249	2495418	bmr	ARO:3003007	bmr	99.74	100.00	M33768.1
CKPLAKGK_1	1376452	1376769	ykkD	ARO:3003064	ykkD	100.00	100.00	AL009126.1
CKPLAKGK_1	1376114	1376452	ykkC	ARO:3003063	ykkC	100.00	100.00	AL009126.1
CKPLAKGK_1	916373	918943	Bacillus subtilis mprF	ARO:3003324	Bsub_mprF	100.00	100.00	AL009126.3
CKPLAKGK_1	604333	605976	vmlR	ARO:3004476	vmlR	100.00	100.00	NC_000964.3
CKPLAKGK_1	338754	339347	tmrB	ARO:3003059	tmrB	100.00	100.00	AL009126.3
CKPLAKGK_1	288251	289684	lmrB	ARO:3002813	lmrB	100.00	100.00	JYFL01000006.1
CKPLAKGK_1	275436	276356	mphK	ARO:3004541	mphK	100.00	100.00	CP010314.1

RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)

ResFinder

Query ID	Begin	End	Resistance gene	Phenotype	Identity	Coverage	Accession no.
CKPLAKGK_1	2735277	2736131	aadK	Streptomycin	100.00	100.00	M26879
CKPLAKGK_1	275436	276356	mph(K)	Spiramycin, Telithromycin	100.00	100.00	NC_000964
CKPLAKGK_1	4251207	4252583	tet(L)	Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline	100.00	100.00	X08034

ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)

AMRFinderPlus

Query ID	Begin	End	Gene symbol	Sequence name	Method	Identity	Coverage	Accession no.
CKPLAKGK_1	2735280	2736131	aadK	aminoglycoside 6-adenylyltransferase AadK	EXACTX	100.00	100.00	WP_003229862.1
CKPLAKGK_1	275436	276353	mphK	macrolide 2'-phosphotransferase MphK	EXACTX	100.00	100.00	WP_003246254.1
CKPLAKGK_1	4248689	4249207	satA	streptothricin N-acetyltransferase SatA	EXACTX	100.00	100.00	WP_003242546.1
CKPLAKGK_1	4251210	4252583	tet(L)	tetracycline efflux MFS transporter Tet(L)	EXACTX	100.00	100.00	WP_003242953.1
CKPLAKGK_1	604336	605976	vmlR	ABC-F type ribosomal protection protein VmlR	EXACTX	100.00	100.00	WP_003234144.1
CKPLAKGK_1	2050934	2053528	rphC	rifamycin-inactivating phosphotransferase RphC	BLASTX	81.15	99.88	WP_087347987.1

AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)

Virulence Factor Database (VFDB)

Query ID	qstart	qend	Gene name	VF name	VF category	Identity	Coverage	Accession no.
CKPLAKGK_1	3761599	3762741	capA	Capsule	Immune modulation	100.00	100.00	NP_391469
CKPLAKGK_1	3763224	3764405	capB	Capsule	Immune modulation	100.00	100.00	NP_391471
CKPLAKGK_1	3762760	3763209	capC	Capsule	Immune modulation	100.00	100.00	NP_391470
CKPLAKGK_1	2004276	2006039	dep/capD	Capsule	Immune modulation	100.00	100.00	NP_389723
CKPLAKGK_1	3355264	3356049	dhbA	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391080
CKPLAKGK_1	3351428	3352366	dhbB	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391077
CKPLAKGK_1	3354042	3355238	dhbC	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391079
CKPLAKGK_1	3352394	3354013	dhbE	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391078
CKPLAKGK_1	3344272	3351408	dhbF	Bacillibactin	Nutritional/Metabolic factor	100.00	100.00	NP_391076
CKPLAKGK_1	2294902	2295543	hlyIII	Hemolysin III	Exotoxin	100.00	100.00	NP_390062

BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)

VirulenceFinder

Query ID	Begin	End	Database	Virulence factor	Protein function	Identity	Coverage	Accession no.
No hit found

VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)

PHI-base

Query ID	qstart	qend	Gene name	e-value	Function	Identity	Coverage	Gene ID
CKPLAKGK_1	132472	133608	tufA	0.0	elongation factor Tu	84.96	95.69	AHM69299
CKPLAKGK_1	18664	19533	pdxS	3.18e-149	encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS	83.79	98.31	ACE61268
CKPLAKGK_1	1689716	1690492	CodY	3.47e-124	nutrient-responsive regulator	81.08	100.00	ACJ80679
CKPLAKGK_1	3609987	3610556	ClpP	2.57e-98	part of proteolytic complex	81.05	97.44	BAB94595

BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)

PlasmidFinder

Query ID	Begin	End	Plasmid	Query / Template	Identity	Accession no.
No hit found

PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)

PlasmidHunter

Query ID	Prediction	Chromosome	Plasmid
CKPLAKGK_1	0.0	1.0	0.0
CKPLAKGK_2	1.0	0.0	1.0

PlasmidHunter v 1.4.5 (Database: May. 2024 release)

Phigaro

Query ID	Begin	End	Transposable	Taxonomy	pVOGs
CKPLAKGK_1	1320167	1346275	False	Myoviridae	VOG4602, VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705
CKPLAKGK_1	2666639	2696883	False	Myoviridae	VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG10055, VOG4870, VOG4589, VOG0749, VOG4685, VOG4688, VOG4773, VOG10227, VOG0796, VOG4673, VOG9587, VOG4693, VOG0189, VOG11060, VOG1309, VOG4600
CKPLAKGK_1	3196003	3225429	False	Siphoviridae	VOG0705, VOG6116, VOG0602, VOG4574, VOG5221, VOG4705, VOG4612, VOG4660, VOG4619, VOG0801, VOG6163, VOG0725, VOG0799, VOG0704, VOG5589, VOG4564, VOG5504, VOG3676, VOG4555, VOG5012, VOG5023, VOG0275, VOG3699, VOG6495, VOG4791
CKPLAKGK_1	3243679	3252505	False	Siphoviridae	VOG10937, VOG5753, VOG6546, VOG1563, VOG2215, VOG4551, VOG0025, VOG0044
CKPLAKGK_2	5173	12905	False	Myoviridae / Siphoviridae	VOG0025, VOG4551, VOG5263, VOG0918, VOG0677, VOG0982
CKPLAKGK_2	65417	123456	True	Myoviridae	VOG5206, VOG11016, VOG4714, VOG0703, VOG9091, VOG4615, VOG7230, VOG0647, VOG5173, VOG3432, VOG9244, VOG5242, VOG3389, VOG0275, VOG8609, VOG8610, VOG5534, VOG6940, VOG7628, VOG7629, VOG6472, VOG6036, VOG6941, VOG6246, VOG6524, VOG6031, VOG5721, VOG7724, VOG5722, VOG4570, VOG0048, VOG0678, VOG7814, VOG5216, VOG0058, VOG9245, VOG10598, VOG8611, VOG7848, VOG10599, VOG0275, VOG3237

Phigaro v2.4.0 (Database: Jan. 2024 release)