Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 9 | 10 | 9 | 16.19 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DAPKMJLA_4 | 101490 | 101807 | ykkD | ARO:3003064 | ykkD | 98.10 | 100.00 | AL009126.1 |
| DAPKMJLA_4 | 101152 | 101490 | ykkC | ARO:3003063 | ykkC | 99.11 | 100.00 | AL009126.1 |
| DAPKMJLA_11 | 40995 | 41915 | mphK | ARO:3004541 | mphK | 98.68 | 100.00 | CP010314.1 |
| DAPKMJLA_32 | 30628 | 31830 | blt | ARO:3003006 | blt | 99.25 | 100.00 | L32599.1 |
| DAPKMJLA_11 | 27662 | 29101 | lmrB | ARO:3002813 | lmrB | 98.74 | 100.42 | JYFL01000006.1 |
| DAPKMJLA_28 | 15581 | 17227 | vmlR | ARO:3004476 | vmlR | 98.36 | 100.18 | NC_000964.3 |
| DAPKMJLA_34 | 9453 | 12023 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.65 | 100.00 | AL009126.3 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| DAPKMJLA_14 | 34916 | 36292 | tet(L) | Doxycycline, Tetracycline, Doxycycline, Tetracycline, Minocycline, Tigecycline | 99.13 | 100.00 | D12567 |
| DAPKMJLA_11 | 40995 | 41915 | mph(K) | Spiramycin, Telithromycin | 98.05 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| DAPKMJLA_14 | 34916 | 36289 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 99.13 | 100.00 | WP_063856081.1 |
| DAPKMJLA_11 | 41007 | 41915 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.68 | 99.02 | WP_003246254.1 |
| DAPKMJLA_28 | 15584 | 17227 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.36 | 100.00 | WP_003234144.1 |
| DAPKMJLA_14 | 38294 | 38812 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.75 | 100.00 | WP_003242546.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| DAPKMJLA_7 | 113972 | 115153 | capB | 0.0 | Capsule | Immune modulation | 99.66 | 100.00 | NP_391471 |
| DAPKMJLA_12 | 36147 | 36932 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.62 | 100.00 | NP_391080 |
| DAPKMJLA_7 | 115636 | 116778 | capA | 0.0 | Capsule | Immune modulation | 99.30 | 100.00 | NP_391469 |
| DAPKMJLA_12 | 32311 | 33249 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.15 | 100.00 | NP_391077 |
| DAPKMJLA_40 | 23585 | 25348 | dep/capD | 0.0 | Capsule | Immune modulation | 98.98 | 100.00 | NP_389723 |
| DAPKMJLA_13 | 97037 | 97678 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 98.91 | 100.00 | NP_390062 |
| DAPKMJLA_7 | 115168 | 115617 | capC | 0.0 | Capsule | Immune modulation | 98.89 | 100.00 | NP_391470 |
| DAPKMJLA_12 | 25155 | 32291 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.61 | 100.00 | NP_391076 |
| DAPKMJLA_12 | 34925 | 36121 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.83 | 100.00 | NP_391079 |
| DAPKMJLA_12 | 33277 | 34896 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 97.10 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| DAPKMJLA_20 | 31777 | 32913 | tufA | 0.0 | elongation factor Tu | 85.75 | 95.69 | AHM69299 |
| DAPKMJLA_45 | 11278 | 10409 | pdxS | 1.12e-151 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| DAPKMJLA_3 | 195983 | 196759 | CodY | 1.68e-125 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| DAPKMJLA_10 | 99297 | 98728 | ClpP | 8.98e-100 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
| DAPKMJLA_27 | 23406 | 23798 | spxA1 | 3.31e-71 | redox-responsive transcription factor | 83.97 | 100.00 | AAF21893 |
| DAPKMJLA_27 | 23406 | 23798 | spx | 1.98e-69 | essential gene which is a thiol/oxidative stress sensor | 80.15 | 100.00 | BAB57159 |
| DAPKMJLA_24 | 20599 | 20841 | SpoVG | 7.52e-39 | RNA-binding protein | 81.48 | 79.41 | AAT02994 |
| DAPKMJLA_51 | 5268 | 5465 | CspA | 2.68e-33 | cold shock protein | 90.91 | 100.00 | CAA62903 |
| DAPKMJLA_13 | 85807 | 85613 | CspD | 7.80e-29 | cold shock protein | 81.54 | 98.48 | CAC99957 |
| DAPKMJLA_5 | 162912 | 163106 | CspB | 1.79e-27 | cold shock protein | 83.08 | 98.48 | CAD00094 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| DAPKMJLA_1 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_2 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_3 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_4 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_5 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_6 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_7 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_8 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_9 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_10 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_11 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_12 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_13 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_14 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_15 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_16 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_17 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_18 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_19 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_20 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_21 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_22 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_23 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_24 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_25 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_26 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_27 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_28 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_29 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_30 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_31 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_32 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_33 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_34 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_35 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_36 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_37 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_38 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_39 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_40 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_41 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_42 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_43 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_44 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_45 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_46 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_47 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_48 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_49 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_50 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_51 | 1.0 | 0.0 | 1.0 |
| DAPKMJLA_52 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_53 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_54 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_55 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_57 | 1.0 | 0.0 | 1.0 |
| DAPKMJLA_58 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_59 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_60 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_61 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_62 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_63 | 1.0 | 0.0 | 1.0 |
| DAPKMJLA_65 | 1.0 | 0.0 | 1.0 |
| DAPKMJLA_66 | 0.0 | 1.0 | 0.0 |
| DAPKMJLA_67 | 1.0 | 0.0 | 1.0 |
| DAPKMJLA_68 | 1.0 | 0.0 | 1.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| DAPKMJLA_4 | 45096 | 71696 | False | Myoviridae | VOG9667, VOG3490, VOG0189, VOG4861, VOG5447, VOG0796, VOG9315, VOG10227, VOG4773, VOG4685, VOG0749, VOG3890, VOG3891, VOG3892, VOG6163, VOG4865, VOG4885, VOG3894, VOG4550, VOG4691, VOG4862, VOG7718, VOG4705 |
| DAPKMJLA_9 | 85067 | 123594 | True | Siphoviridae | VOG0641, VOG4705, VOG6243, VOG4612, VOG4626, VOG4772, VOG0801, VOG5910, VOG0541, VOG4586, VOG0704, VOG9583, VOG0204, VOG10223, VOG4553, VOG4568, VOG4556, VOG3607, VOG1886, VOG4581, VOG0713, VOG4841, VOG0275, VOG0198, VOG0044, VOG0102, VOG0397, VOG5836, VOG0245, VOG1336, VOG6587, VOG0189, VOG4566, VOG7581, VOG9667, VOG9667, VOG1563, VOG0275, VOG0275 |
Phigaro v2.4.0 (Database: Jan. 2024 release)