Probiotic potential risk score
| ARGs | VFs | PGs | PPRS |
|---|---|---|---|
| 13 | 10 | 4 | 16.88 |
PPRS is classified as low-risk (≤4), medium-risk (4-6), and high-risk (≥6).
Antibiotic resistance genes
Comprehensive Antibiotic Resistance Database (CARD)
| Query ID | Begin | End | ARO name | ARO accession | CARD name | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ABANGKPK_1 | 3758186 | 3758779 | tmrB | ARO:3003059 | tmrB | 98.48 | 100.00 | AL009126.3 |
| ABANGKPK_1 | 3562854 | 3564497 | vmlR | ARO:3004476 | vmlR | 98.54 | 100.00 | NC_000964.3 |
| ABANGKPK_1 | 3385843 | 3387276 | lmrB | ARO:3002813 | lmrB | 99.37 | 100.00 | JYFL01000006.1 |
| ABANGKPK_1 | 3373030 | 3373950 | mphK | ARO:3004541 | mphK | 98.69 | 100.00 | CP010314.1 |
| ABANGKPK_1 | 3113927 | 3116497 | Bacillus subtilis mprF | ARO:3003324 | Bsub_mprF | 99.77 | 100.00 | AL009126.3 |
| ABANGKPK_1 | 2565166 | 2565504 | ykkC | ARO:3003063 | ykkC | 100.00 | 100.00 | AL009126.1 |
| ABANGKPK_1 | 2564849 | 2565166 | ykkD | ARO:3003064 | ykkD | 99.05 | 100.00 | AL009126.1 |
| ABANGKPK_1 | 1660920 | 1662089 | bmr | ARO:3003007 | bmr | 99.49 | 100.00 | M33768.1 |
| ABANGKPK_1 | 1463838 | 1465040 | blt | ARO:3003006 | blt | 99.75 | 100.00 | L32599.1 |
| ABANGKPK_1 | 1435141 | 1435995 | aadK | ARO:3002627 | aadK | 98.24 | 100.00 | AL009126.1 |
RGI v6.0.3 (Database: CARD Variants v4.0.2, Nov. 2023 release)
ResFinder
| Query ID | Begin | End | Resistance gene | Phenotype | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|
| ABANGKPK_1 | 1435141 | 1435993 | aadK | Streptomycin | 98.59 | 99.77 | M26879 |
| ABANGKPK_1 | 1024238 | 1025614 | tet(L) | Doxycycline, Tetracycline, Minocycline, Tigecycline, Doxycycline, Tetracycline | 98.40 | 100.00 | X08034 |
| ABANGKPK_1 | 3373030 | 3373950 | mph(K) | Spiramycin, Telithromycin | 98.37 | 100.00 | NC_000964 |
ResFinder v4.6.0 (Database: resfinder_db, Aug. 2024 release)
AMRFinderPlus
| Query ID | Begin | End | Gene symbol | Sequence name | Method | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| ABANGKPK_1 | 3373030 | 3373947 | mphK | macrolide 2'-phosphotransferase MphK | BLASTX | 98.69 | 100.00 | WP_003246254.1 |
| ABANGKPK_1 | 3562854 | 3564494 | vmlR | ABC-F type ribosomal protection protein VmlR | BLASTX | 98.54 | 100.00 | WP_003234144.1 |
| ABANGKPK_1 | 1024241 | 1025614 | tet(L) | tetracycline efflux MFS transporter Tet(L) | BLASTX | 98.47 | 100.00 | WP_003242953.1 |
| ABANGKPK_1 | 1435141 | 1435992 | aadK | aminoglycoside 6-adenylyltransferase AadK | BLASTX | 98.24 | 100.00 | WP_003229862.1 |
| ABANGKPK_1 | 1021767 | 1022285 | satA | streptothricin N-acetyltransferase SatA | BLASTX | 90.17 | 100.00 | WP_003242546.1 |
| ABANGKPK_1 | 1963354 | 1965951 | rphC | rifamycin-inactivating phosphotransferase RphC | BLASTX | 80.83 | 100.00 | WP_087347987.1 |
AMRFinderPlus v4.0.3 (Database: Oct. 2024 release)
Virulence factors
Virulence Factor Database (VFDB)
| Query ID | qstart | qend | Gene name | e-value | VF name | VF category | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|---|
| ABANGKPK_1 | 529869 | 531050 | capB | 0.0 | Capsule | Immune modulation | 99.58 | 100.00 | NP_391471 |
| ABANGKPK_1 | 113020 | 113805 | dhbA | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.49 | 100.00 | NP_391080 |
| ABANGKPK_1 | 528244 | 529386 | capA | 0.0 | Capsule | Immune modulation | 99.47 | 100.00 | NP_391469 |
| ABANGKPK_1 | 109184 | 110122 | dhbB | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.47 | 100.00 | NP_391077 |
| ABANGKPK_1 | 1860720 | 1861361 | hlyIII | 0.0 | Hemolysin III | Exotoxin | 99.38 | 100.00 | NP_390062 |
| ABANGKPK_1 | 529405 | 529854 | capC | 0.0 | Capsule | Immune modulation | 99.33 | 100.00 | NP_391470 |
| ABANGKPK_1 | 2014817 | 2016580 | dep/capD | 0.0 | Capsule | Immune modulation | 99.21 | 100.00 | NP_389723 |
| ABANGKPK_1 | 102028 | 109164 | dhbF | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 99.16 | 100.00 | NP_391076 |
| ABANGKPK_1 | 111798 | 112994 | dhbC | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.83 | 100.00 | NP_391079 |
| ABANGKPK_1 | 110150 | 111769 | dhbE | 0.0 | Bacillibactin | Nutritional/Metabolic factor | 98.46 | 100.00 | NP_391078 |
BLASTN v2.16.0 (Database: VFDB, Dec. 2024 release)
VirulenceFinder
| Query ID | Begin | End | Database | Virulence factor | Protein function | Identity | Coverage | Accession no. |
|---|---|---|---|---|---|---|---|---|
| No hit found | ||||||||
VirulenceFinder v2.0. 4 (Database: virulencefinder_db, Feb . 2024 release)
Pathogenic genes
PHI-base
| Query ID | qstart | qend | Gene name | e-value | Function | Identity | Coverage | Gene ID |
|---|---|---|---|---|---|---|---|---|
| ABANGKPK_1 | 3901207 | 3902343 | tufA | 0.0 | elongation factor Tu | 84.96 | 95.69 | AHM69299 |
| ABANGKPK_1 | 4032970 | 4033839 | pdxS | 3.04e-149 | encodes the putative pyridoxal 5'-phosphate synthase subunit PdxS | 83.79 | 98.31 | ACE61268 |
| ABANGKPK_1 | 2255382 | 2254606 | CodY | 3.32e-124 | nutrient-responsive regulator | 81.08 | 100.00 | ACJ80679 |
| ABANGKPK_1 | 364744 | 365313 | ClpP | 2.46e-98 | part of proteolytic complex | 81.05 | 97.44 | BAB94595 |
BLASTX v2.16.0 (Database: PHI base v4.16, May. 2024 release)
Plasmid
PlasmidFinder
| Query ID | Begin | End | Plasmid | Query / Template | Identity | Accession no. |
|---|---|---|---|---|---|---|
| No hit found | ||||||
PlasmidFinder v 2.1.6 (Database: plasmidfinder_db v 2.2.0 , Nov. 2024 release)
PlasmidHunter
| Query ID | Prediction | Chromosome | Plasmid |
|---|---|---|---|
| ABANGKPK_1 | 0.0 | 1.0 | 0.0 |
PlasmidHunter v 1.4.5 (Database: May. 2024 release)
Prophage
Phigaro
| Query ID | Begin | End | Transposable | Taxonomy | pVOGs |
|---|---|---|---|---|---|
| ABANGKPK_1 | 2027103 | 2053206 | False | Siphoviridae | VOG0136, VOG5221, VOG7896, VOG3390, VOG4612, VOG4705, VOG3390, VOG4626, VOG4619, VOG0801, VOG6163, VOG5910, VOG0541, VOG4586, VOG0704, VOG0539, VOG4589, VOG0204, VOG5361, VOG4553, VOG4568, VOG4556, VOG1886, VOG4581, VOG8507 |
| ABANGKPK_1 | 2059295 | 2065579 | False | Siphoviridae | VOG0535, VOG1638, VOG5651, VOG0650, VOG4552, VOG6495, VOG9667, VOG4602 |
| ABANGKPK_1 | 2595315 | 2622117 | False | Myoviridae | VOG4705, VOG7718, VOG4862, VOG4691, VOG4550, VOG3894, VOG4885, VOG4865, VOG6163, VOG3892, VOG3891, VOG3890, VOG0749, VOG4685, VOG4773, VOG10227, VOG0796, VOG5447, VOG4861, VOG0189, VOG3490, VOG9667 |
| ABANGKPK_1 | 3984872 | 3994461 | True | Siphoviridae | VOG0275, VOG0275, VOG4602, VOG8483, VOG6495, VOG8241, VOG3549, VOG1878, VOG0083, VOG1309, VOG1292 |
Phigaro v2.4.0 (Database: Jan. 2024 release)